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抑制丙肝病毒复制和转录的一种新方法

, PP. 473-477

Keywords: 丙肝病毒,复制和转录,Ago2蛋白,电化学分析,生物传感器

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Abstract:

丙肝病毒是一种正链RNA病毒,它是导致人类肝脏疾病的重要因素.本研究中,通过引入一段体外的RNA,使其与丙肝病毒基因组、miR-122三者碱基互补配对,从而激活Ago2蛋白的内切酶活性,导致HCV基因组被切割,进而从其与Ago2蛋白和miR-122所形成的复合物中释放出来.由于失去了Ago2蛋白和miR-122的保护作用,释放出来的HCV基因组很容易被降解,因而可有效抑制其复制和转录.该研究为抑制HCV的复制和转录提供了新的思路,对于丙肝的治疗将具有意义.

References

[1]  1 Khaliq S, Jahan S, Ijaz B, et al. Inhibition of hepatitis C virus genotype 3a by siRNAs targeting envelope genes. Arch Virol, 2011, 156: 433-442
[2]  2 Chevalier C, Saulnier A, Benureau Y, et al. Inhibition of hepatitis C virus infection in cell culture by small interfering RNAs. Mol Ther, 2007, 15: 1452-1462
[3]  3 Chang B, Lee C H, Lee J H, et al. Comparative analysis of intracellular inhibition of hepatitis C virus replication by small interfering RNAs. Biotechnol Lett, 2010, 32: 1231-1237
[4]  9 Tan G S, Garchow B G, Liu X, et al. Expanded RNA-binding activities of mammalian Argonaute 2. Nucleic Acids Res, 2009, 37: 7533-7545
[5]  10 Rand T A, Ginalski K, Grishin N V, et al. Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity. Proc Natl Acad Sci USA, 2004, 101: 14385-14389
[6]  11 Liu Y, Tan H, Tian H, et al. Autoantigen La promotes efficient RNAi, antiviral response, and transposon silencing by facilitating multiple-turnover RISC catalysis. Mol Cell, 2011, 44: 502-508
[7]  12 Schirle N T, MacRae I J. The crystal structure of human Argonaute2. Science, 2012, 336: 1037-1040
[8]  13 Voinnet O. Origin, biogenesis, and activity of plant microRNAs. Cell, 2009, 136: 669-687
[9]  4 Clark V C, Peter J A, Nelson D R. New therapeutic strategies in HCV: second-generation protease inhibitors. Liver Int, 2013, 33: 80-84
[10]  5 Ariumi Y, Kuroki M, Kushima Y, et al. Hepatitis C virus hijacks P-body and stress granule components around lipid droplets. J Virol, 2011, 85: 6882-6892
[11]  6 Jopling C L, Schutz S, Sarnow P. Position-dependent function for a tandem microRNA miR-122-binding site located in the hepatitis C virus RNA genome. Cell Host Microbe, 2008, 4: 77-85
[12]  7 Berezhna S Y, Supekova L, Sever M J, et al. Dual regulation of hepatitis C viral RNA by cellular RNAi requires partitioning of Ago2 to lipid droplets and P-bodies. RNA, 2011, 17: 1831-1845
[13]  8 Liu J, Carmell M A, Rivas F V, et al. Argonaute 2 is the catalytic engine of mammalian RNAi. Science, 2004, 305: 1437-1441
[14]  14 Shimakami T, Yamane D, Jangra R K, et al. Stabilization of hepatitis C virus RNA by an Ago2-miR-122 complex. Proc Natl Acad Sci USA, 2012, 109: 941-946
[15]  15 Young D D, Connelly C M, Grohmann C, et al. Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma. J Am Chem Soc, 2010, 132: 7976-7981
[16]  16 Henke J I, Goergen D, Zheng J, et al. MicroRNA-122 stimulates translation of hepatitis C virus RNA. EMBO J, 2008, 27: 3300-3310
[17]  17 Lanford R E, Hildebrandt-Eriksen E S, Petri A, et al. Therapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infection. Science, 2010, 327: 198-201
[18]  18 Wilson J A, Zhang C, Huys A, et al. Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol, 2011, 85: 2342-2350
[19]  19 Yang N, Cao Y, Han P, et al. Tools for investigation of the RNA endonuclease activity of mammalian Argonaute 2 protein. Anal Chem, 2012, 84: 2492-2497
[20]  20 Roberts A P, Lewis A P, Jopling C L. MiR-122 activates hepatitis C virus translation by a specialized mechanism requiring particular RNA components. Nucleic Acids Res, 2011, 39: 7716-7729
[21]  21 Jopling C L, Yi M, Lancaster A M, et al. Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science, 2005, 309: 1577-1581
[22]  22 Yin H, Zhou Y, Zhang H, et al. Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system. Biosens Bioelectron, 2012, 33: 247-253
[23]  23 Huntzinger E, Izaurralde E. Gene silencing by microRNAs: contributions of translational repression and mRNA decay. Nat Rev Genet, 2011, 12: 99-110
[24]  24 Hun X, Wang Z P. L-Argininamide biosensor based on S1 nuclease hydrolysis signal amplification. Microchim Acta, 2012, 176: 209-216

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