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磷脂酰肌醇蛋白聚糖-3特异性抗体对肝细胞癌临床诊断的初步应用

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Keywords: 磷脂酰肌醇蛋白,聚糖-3,7D11单克隆抗体,肝细胞癌,免疫组化

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Abstract:

磷脂酰肌醇蛋白聚糖-3(GPC3)对肝细胞癌(HCC)的诊断具有很高的敏感性和特异性,是具有前景的肿瘤标志物.本文的目的是应用抗人-GPC3单克隆抗体7D11(7D11McAb),通过免疫组化(IHC)的方法检测HCC组织中的GPC3蛋白,并初步评价其诊断HCC的临床应用价值.通过对HCC病理组织、正常肝脏组织和胆管细胞癌(ICC)病理组织进行免疫组化染色来评价7D11McAb的可应用性.同时,应用7D11McAb作为检测抗体对40例HCC,7例ICC和50例正常人的血清GPC3进行检测.免疫组化结果显示,GPC3蛋白在85%(34/40)HCC组织高表达,而在正常人和ICC病人的组织中阳性率均为0%(0/50和0/7).血清学检测结果显示,GPC3在HCC,正常人和ICC病人的血清中阳性率分别为:42.5%(17/40),0%(0/50%)和0%(0/7).7D11McAb的制备为GPC3能大规模应用于临床奠定了基础.

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