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1999~2013年山东H9N2亚型禽流感病毒HA基因的演化和HI抗原性差异分析

, PP. 190-199

Keywords: H9N2,HA基因,系谱分群,HI交叉抑制,相关性

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Abstract:

利用生物信息学方法和血凝交叉抑制(HI)试验比较研究了山东地区低致病性禽流感H9N2亚型病毒的分子遗传变异和抗原性变化.对1999~2013年在山东地区分离并鉴定的35株H9N2病毒进行了HA基因测序和同源性分析,绘制了系统发育树.同源性比较显示,同一时期的毒株同源性较高,而不同年代的H9N2流行毒相差较大,如2010~2013年的H9N2流行毒之间核苷酸(氨基酸)同源性为94.5%~100%(96.1%~100%),但与疫苗株SD-6的核苷酸(氨基酸)同源性已降至90.0%~92.5%(91.8%~95.0%).系统发育树显示,35株H9N2病毒均属欧亚谱系,其中28株属于S2-like亚群,表明该类型毒株是山东地区最主要的流行株.重要氨基酸位点分析表明,2010年以后的H9N2流行毒,其HA蛋白裂解位点基序为PSRSSR↓GL,334位点均由原来的A变为S;与受体结合位点相关的234位点,则由Q变为L.选择不同亚群的H9N2代表毒株制备单因子血清,进行HI交叉抑制实验,结果发现,H9N2不同毒株之间均可发生一定的交叉反应,但相关指数最低已降至0.31,表明病毒已出现明显的抗原性变化.本研究证实,山东H9N2亚型禽流感病毒在分子和抗原水平上均已发生一定程度的变异.

References

[1]  1 Alexander D J. Summary of avian influenza activity in Europe, Asia, Africa, and Australasia, 2002~2006. Avian Dis, 2007, 51: 161-166
[2]  2 Cox N J, Subbarao K. Global epidemiology of influenza: past and present. Annu Rev Med, 2000, 51: 407-421
[3]  3 Iqbal M, Yaqub T, Mukhtar N, et al. Infectivity and transmissibility of H9N2 avian influenza virus in chickens and wild terrestrial birds. Vet Res, 2013, 44: 100
[4]  4 王友令, 袁小远, 徐怀英, 等. 高抗体水平肉种鸡H9N2的分离鉴定及HA基因序列分析. 华北农学报, 2010, 3: 23-27
[5]  5 Wang J Y, Ren J J, Liu W H, et al. Complete genome sequence of a new H9N2 avian influenza virus isolated in China. Genome Announc, 2013, 1: e00261-13
[6]  6 Zhao M, Liu Q, Sun Q, et al. Full genome sequence of a natural reassortant H9N2 avian influenza virus isolated from domestic ducks in Jiangsu Province, China. Genome Announc, 2013, 1: e00463-13
[7]  7 申翰钦, 曾凡桂, 严专强, 等. 广东一株重组H9N2亚型禽流感病毒的鉴定及全基因组序列分析. 中国预防兽医学报, 2013, 35: 607-612
[8]  8 Post J, de Geus E D, Vervelde L, et al. Systemic distribution of different low pathogenic avian influenza (LPAI) viruses in chicken. Virol J, 2013, 10: 23
[9]  9 Srinivasan K, Raman R, Jayaraman A, et al. Quantitative characterization of glycan-receptor binding of H9N2 influenza A virus hemagglutinin. PLoS One, 2013, 8: e59550
[10]  10 陈陆, 郑鹿平, 赵军, 等. H9N2亚型禽流感病毒HA蛋白S145N变异株致病性及抗原特性. 畜牧兽医学报, 2012, 43: 82-89
[11]  11 岳华, 汤承, 杨发龙, 等. 中国大陆H9N2亚型禽流感病毒HA基因遗传分析及抗原相关性研究. 西南民族大学学报(自然科学版), 2010, 2: 200-205
[12]  12 赵军, 柴丽娜, 王泽霖. 1998~2008年中国中部H9N2亚型AIV分离毒株HA基因的进化分析. 病毒学报, 2011, 2: 122-128
[13]  13 World Organization for Animal Health (OIE). “Newcastle disease,” in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). Paris: OIE (Office International des Epizooties), 2008. 576-589
[14]  14 刘娟, 张伟, 徐怀英, 等. H9N2禽流感不同毒株鸡胚交叉中和指数及其与HA基因氨基酸同源性相关的研究. 华北农学报, 2013, 28: 23-28
[15]  15 Li C, Yu K, Tian G, et al. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China. Virology, 2005, 340: 70-83
[16]  16 Lee Y J, Shin J Y, Song M S, et al. Continuing evolution of H9 influenza viruses in Korean poultry. Virology, 2007, 359: 313-323
[17]  17 Stephenson I, Nicholson K G, Gluck R, et al. Safety and antigenicity of whole virus and subunit influenza A/Hong Kong/1073/99 (H9N2) vaccine in healthy adults: phase I randomised trial. Lancet, 2003, 362: 1959-1966
[18]  18 娄本红, 朱秀同, 孙贝贝, 等. 抗体选择压作用下H9N2亚型禽流感病毒HA基因的变异. 微生物学报, 2009, 7: 955-957
[19]  27 Bonfante F, Patrono L V, Aiello R, et al. Susceptibility and intra-species transmission of the H9N2 G1 prototype lineage virus in Japanese quail and turkeys. Vet Microbiol, 2013, 165: 177-183
[20]  28 Chen Q, Huang S, Chen J, et al. NA proteins of influenza A viruses H1N1/2009, H5N1, and H9N2 show differential effects on infection initiation, virus release, and cell-cell fusion. PLoS One, 2013, 8: e54334
[21]  29 Qin G, Liu Y, Zheng J, et al. Type 1 responses of human Vg9Vd2 T cells to influenza A viruses. J Virol, 2011, 85: 10109-10116
[22]  30 Uyeki T M, Nguyen D C, Rowe T, et al. Seroprevalence of antibodies to avian influenza A (H5) and A (H9) viruses among market poultry workers, Hanoi, Vietnam, 2001. PLoS One, 2012, 7: e43948
[23]  31 Takakuwa H, Yamashiro T, Le M Q, et al. The characterization of low pathogenic avian influenza viruses isolated from wild birds in northern Vietnam from 2006 to 2009. Comp Immunol Microb, 2013, 36: 581-590
[24]  32 Zhu G, Wang R, Xuan F, et al. Characterization of recombinant H9N2 influenza viruses isolated from wild ducks in China. Vet Microbiol, 2013, 166: 327-336
[25]  33 Sun Y, Qin K, Wang J, et al. High genetic compatibility and increased pathogenicity of reassortants derived from avian H9N2 and pandemic H1N1/2009 influenza viruses. Proc Natl Acad Sci USA, 2011, 108: 4164-4169
[26]  19 Huang Y, Hu B, Wen X, et al. Diversified reassortant H9N2 avian influenza viruses in chicken flocks in northern and eastern China. Virus Res, 2010, 1: 26-32
[27]  20 Meng D, Hui Z, Yang J, et al. Reduced egg production in hens associated with avian influenza vaccines and formalin levels. Avian Dis, 2009, 53: 16-20
[28]  21 Zhang P, Tang Y, Liu X, et al. Characterization of H9N2 influenza viruses isolated from vaccinated flocks in an integrated broiler chicken operation in eastern China during a 5 year period (1998~2002). J Gen Virol, 2008, 89: 3102-3112
[29]  22 杨汉春. 动物免疫学. 北京: 中国农业大学出版社, 1996. 146-147
[30]  23 Bano S, Naeem K, Malik S A. Evaluation of pathogenic potential of avian influenza virus serotype H9N2 in chickens. Avian Dis, 2003, 47: 817-822
[31]  24 Coman A, Maftei D N, Krueger W S, et al. Serological evidence for avian H9N2 influenza virus infections among Romanian agriculture workers. J Infect Public Health, 2013, 6: 438-447
[32]  25 Lin Y P, Shaw M, Gregory V, et al. Avian-to-human transmission of H9N2 subtype influenza a viruses: relationship between H9N2 and H5N1 human isolates. Proc Natl Acad Sci USA, 2000, 97: 9654-9658
[33]  26 张增峰, 樊晓晖, 陈晓燕. 禽流感病毒H9N2在人肺组织的复制. 病毒学报, 2013, 29: 206-210

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