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中国美利奴羊MHC-DRB1exon2单倍型构建及与布鲁氏菌病易感性关联分析

DOI: 10.1360/N052013-00069, PP. 495-501

Keywords: 布鲁氏菌病,MHC-DRB1exon2,连锁不平衡,单倍型,易感性

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Abstract:

为研究中国美利奴羊MHC-DRB1基因exon2单倍型与布鲁氏菌易感性的关联性,本实验采用PCR直接测序法对40例布鲁氏菌血清检测阳性和阴性个体MHC-DRB1exon2的单核苷酸多态性(SNPs)进行检测,而后运用SHEsis在线软件对筛选的SNPs构建单倍型并进行单倍型关联分析.结果显示,在270bp的序列内共检测到41个SNPs,经Hardy-Weinberg平衡检测筛选出符合条件的SNPs有29个,连锁不平衡发现9个连锁不平衡域,而且每个block中的SNPs两两之间存在强连锁不平衡.单倍型分析显示,由于连锁不平衡存在,仅构建9种单倍型,其中只有Hap8和Hap9两种单倍型在病例-对照组中比较差异有统计学意义(P<0.05).

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