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节肢动物Kv2基因A~IRNA编辑位点的鉴定和进化分析及机制研究

DOI: 10.1360/052014-52, PP. 397-405

Keywords: 节肢动物门,RNA编辑,趋同进化,RNA二级结构,Kv2

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Abstract:

A~IRNA编辑可以改变氨基酸编码而增加蛋白质的多样性,但是其分子机制和在进化中的特征依然不清楚.通过对节肢动物门5个纲的物种Kv2基因RNA编辑的研究,检测到17个A~IRNA编辑位点,由保守和物种特异的编辑位点构成,其中编辑位点15(I/V)起源于4.5亿年前,是迄今为止非脊椎动物中最保守的;同时还发现一些A~IRNA编辑位点具有趋同进化现象.在此基础上,通过共转染实验表明了果蝇Kv2基因RNA编辑是RNA编辑酶和由外显子形成的RNA二级结构相互作用的结果,暗示一些外显子除了编码蛋白质的功能,本身也具有重要的基因表达调控功能.

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