全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

白鲫×红鲫杂交后代的遗传变异

, PP. 371-380

Keywords: 日本白鲫,红鲫,杂交,遗传变异

Full-Text   Cite this paper   Add to My Lib

Abstract:

以雌性日本白鲫和雄性红鲫为亲本进行杂交,获得了杂交子代合方鲫.对该杂交子代及其亲本的形态特征、繁殖特性和遗传特性进行了较系统的研究.合方鲫的体色为青灰色,外形特征介于父母本之间,表现出杂交特征.合方鲫性腺发育正常,雌雄两性可育,并且具有较高的受精率(90.2%)和孵化率(80.5%),为新品系的繁衍和群体扩大奠定了基础.合方鲫的染色体数为2n=100,DNA含量与亲本一致,是一种二倍体鱼.合方鲫的45SrDNA的ITS区包括ITS-1,5.8S和ITS-2三部分,总长度为884bp,与亲本序列相似度较高并表现出偏向于父本遗传的特征.同时,杂交子代序列兼有双亲特异碱基位点,表现出重组现象.合方鲫5SrDNA的NTS区具有3种长度类型,序列全长为654bp,与母本相似度为94.2%,与父本似度为95.1%,杂交子代序列具有明显的重组和突变特征,这些基因重组和突变是品种改良和新品系形成的遗传基础.本研究证明了合方鲫在遗传组成和外形方面都与其亲本有本质区别,是一种有杂种特性的后代,该杂交品种的形成在鱼类遗传育种方面具有重要意义.

References

[1]  1 Bartley D M, Rana K, Immink A J. The use of inter-specific hybrids in aquaculture and fisheries. Rev Fish Biol Fisher, 2001, 10: 325-337
[2]  2 张卓慧, 陈婕, 黎玲, 等. 动物远缘杂交研究进展. 中国科学: 生命科学, 2014, 44: 161-174
[3]  3 Liu S J, Qin Q B, Xiao J, et al. The formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance. Genetics, 2007, 176: 1023-1034
[4]  4 Liu S J, Liu Y, Zhou G J, et al. The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization. Aquaculture, 2001, 192: 171-186
[5]  5 Qin Q B, Wang Y D, Wang J, et al. The autotetraploid fish derived from hybridization of carassius auratus red var. (Female)×Megalobrama amblycephala (Male). Biol Reprod, 2014, 91: 93
[6]  6 Xiao J, Kang X W, Xie L H, et al. The fertility of the hybrid lineage derived from female Megalobrama amblycephala×male Culter alburnus. Anim Reprod Sci, 2014, 152: 61-70
[7]  7 Hu J, Liu S J, Xiao J, et al. Characteristics of diploid and triploid hybrids derived from female Megalobrama amblycephala Yih×male Xenocypris davidi Bleeke. Aquaculture, 2012, 364-365: 157-164
[8]  8 He W G, Qin Q B, Liu S J, et al. Organization and variation analysis of 5S rDNA in different ploidy-level hybrids of red crucian carp×copmouth culter. PLoS One, 2012, 7: e38976
[9]  9 王静, 覃钦博, 陈松, 等. 新型高背型鲫鱼的形成及其生物学特征研究. 中国科学C辑: 生命科学, 2008, 38: 635-642
[10]  10 陈松, 王静, 刘少军, 等. 改良三倍体鲫鱼的生物学特性研究. 中国科学C辑: 生命科学, 2009, 39: 479-484
[11]  11 Luo K K, Xiao J, Liu S J, et al. Massive production of all-female diploids and triploids in the crucian carp. Int J Biol Sci, 2011, 7: 487-495
[12]  12 刘筠. 中国养殖鱼类繁殖生理学. 北京: 农业出版社, 1993. 22-30
[13]  13 Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989
[14]  14 Murakami M, Fujitani H. Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi). Genes Genet Syst, 1998, 73: 9-20
[15]  15 Hall T A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 1999, 41: 95-98
[16]  16 Thompson J D, Higgins D G, Gibson T J. CLUSTALW: improving thesensitivity of progressive multiple sequence alignment through sequenceweighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res, 1994, 22: 4673-4680
[17]  17 Barton N H. The role of hybridization in evolution. Mol Ecol, 2001, 10: 551-568
[18]  18 Mallet J. Hybrid speciation. Nature, 2007, 446: 279-283
[19]  19 Seehausen O. Hybridization and adaptive radiation. Trends Ecol Evol, 2004, 19: 198-207
[20]  20 刘少军. 远缘杂交导致不同倍性鱼的形成. 中国科学: 生命科学, 2010, 40: 104-114
[21]  21 Stelkens R, Seehausen O. Genetic distance between species predicts novel trait expression in their hybrids. Evolution, 2009, 63: 884-897
[22]  22 黎玲, 钟泽州, 曾鸣, 等. 不同倍性鱼肌间骨的比较分析. 中国科学: 生命科学, 2013, 43: 189-200
[23]  23 Murakami M, Matsuba C, Fujitani H. The maternal origins of the triploid ginbuna (Carassius auratus langsdorfi): phylogenetic relationships within the C. auratus taxa by partial mitochondrial D-loop sequencing. Genes Genet Syst, 2001, 76: 25-32
[24]  24 刘少军. 鱼类远缘杂交. 北京: 科学出版社, 2014. 6-18
[25]  25 Luo J, Gao Y, Ma W, et al. Tempo and mode of recurrent polyploidization in the Carassius auratus species complex (Cypriniformes, Cyprinidae). Heredity, 2014, 112: 415-427
[26]  26 You C P, Zhao R R, Hu J, et al. Inheritance of the complete mitochondrial genomes in three different ploidy fishes. Curr Mol Med, 2014, 14: 1322-1330
[27]  27 Allen S K, Wattendorf R J. Triploid grass carp: status and management implications. Fisheries, 1987, 12: 20-24

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133