Soil and surface water contamination by used lubricating oil is a common occurrence in most developing countries. This has been shown to have harmful effects on the environment and human beings at large. Bioremediation can be an alternative green technology for remediation of such hydrocarbon-contaminated soil. Bioremediation of soil contaminated with 5% and 15% (w/w) used lubricating oil and amended with 10% brewery spent grain (BSG), banana skin (BS), and spent mushroom compost (SMC) was studied for a period of 84 days, under laboratory condition. At the end of 84 days, the highest percentage of oil biodegradation (92%) was recorded in soil contaminated with 5% used lubricating oil and amended with BSG, while only 55% of oil biodegradation was recorded in soil contaminated with 15% used lubricating oil and amended with BSG. Results of first-order kinetic model to determine the rate of biodegradation of used lubricating oil revealed that soil amended with BSG recorded the highest rate of oil biodegradation (0.4361 day？1) in 5% oil pollution, while BS amended soil recorded the highest rate of oil biodegradation (0.0556 day？1) in 15% oil pollution. The results of this study demonstrated the potential of BSG as a good substrate for enhanced remediation of hydrocarbon contaminated soil at low pollution concentration. 1. Introduction Contamination of soil by used lubricating oil is rapidly increasing due to global increase in the usage of petroleum products [1]. Environmental pollution with petroleum and petrochemical products has attracted much attention in recent decades. The presence of different types of automobiles and machinery has resulted in an increase in the use of lubricating oil. Spillage of used motor oils such as diesel or jet fuel contaminates our natural environment with hydrocarbon [2]. Hydrocarbon contamination of the air, soil, and freshwater especially by PAHs attracts public attention because many PAHs are toxic, mutagenic, and carcinogenic [3–5]. Prolonged exposure to high oil concentration may cause the development of liver or kidney disease, possible damage to the bone marrow, and an increased risk of cancer [6–8]. In addition, PAHs have a widespread occurrence in various ecosystems that contribute to the persistence of these compounds in the environment [9]. The illegal dumping of used motor oil is an environmental hazard with global ramifications [10]. Used motor oil contains metals and heavy polycyclic aromatic hydrocarbons (PAHs) that could contribute to chronic hazards including mutagenicity and carcinogenicity [11, 12]. Lack of
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