A single-step environmental friendly approach is employed to synthesize silver nanoparticles. The biomolecules found in plants induce the reduction of Ag+ ions from silver nitrate to silver nanoparticles (AgNPs). UV-visible spectrum of the aqueous medium containing silver ions demonstrated a peak at 425?nm corresponding to the plasmon absorbance of silver nanoparticles. Transmission electron microscopy (TEM) showed the formation of well-dispersed silver nanoparticles in the range of 5–20?nm. X-ray diffraction (XRD) spectrum of the AgNPs exhibited 2θ values corresponding to the silver nanocrystal. The process of reduction is extracellular and fast which may lead to the development of easy biosynthesis of silver nanoparticles. Plants during glycolysis produce a large amount of H+ ions along with NAD which acts as a strong redoxing agent; this seems to be responsible for the formation of AgNPs. Water-soluble antioxidative agents like ascorbic acids further seem to be responsible for the reduction of AgNPs. These AgNPs produced show good antimicrobial activity against common pathogens. 1. Introduction Nanoparticles with controlled size and composition are of fundamental and technological interest as they provide solutions to technological and environmental challenges in the areas of solar energy conversion, catalysis, medicine, and water treatment. Thus, production and application of nanomaterials from 1 to 100 nanometers (nm) is an emerging field of research [1, 2]. Global warming and climate change have induced a worldwide awareness and effort to reduce generated hazardous wastes. Thus, “Green” chemistry and chemical processes are progressively being integrated in science and industry for sustainable development [3]. Nanomaterials due to their sheer size show unique and considerably changed physical, chemical, and biological properties compared to their macro scale counterparts [4]. Gold, silver, and copper have been used mostly for the synthesis of stable dispersions of nanoparticles, which are useful in areas of photography, catalysis, biological labeling, photonics, optoelectronics, and surface-enhanced Raman scattering (SERS) detection [5–7]. Biological methods are considered safe and ecologically sound for the nanomaterial fabrication as an alternative to conventional physical and chemical methods. Biological routes to the synthesis of these particles have been proposed by exploiting microorganisms [8–12] and by vascular plants [13–22]. The functions of these materials depend on their composition and structure. Plants have been reported to be used
References
[1]
J. A. Dahl, B. L. S. Maddux, and J. E. Hutchison, “Toward greener nanosynthesis,” Chemical Reviews, vol. 107, no. 6, pp. 2228–2269, 2007.
[2]
J. E. Hutchison, “Greener nanoscience: a proactive approach to advancing applications and reducing implications of nanotechnology,” ACS Nano, vol. 2, no. 3, pp. 395–402, 2008.
[3]
P. T. Anstas and J. Warner, Green Chemistry: Theory and Practice, Oxford University Press, New York, NY, USA, 1998.
[4]
L.-S. Li, J. Hu, and A. P. Alivistos, “Band gap variation of size- and shape-controlled colloidal CdSe quantum rods,” Nano Letters, vol. 1, no. 7, pp. 349–351, 2001.
[5]
I. Hussain, M. Brust, A. J. Papworth, and A. I. Cooper, “Preparation of acrylate-stabilized gold and silver hydrosols and gold-polymer composite films,” Langmuir, vol. 19, no. 11, pp. 4831–4835, 2003.
[6]
A. M. Smith, H. Duan, M. N. Rhyner, G. Ruan, and S. Nie, “A systematic examination of surface coatings on the optical and chemical properties of semiconductor quantum dots,” Physical Chemistry Chemical Physics, vol. 8, no. 33, pp. 3895–3903, 2006.
[7]
G. J. Kearns, E. W. Foster, and J. E. Hutchison, “Substrates for direct imaging of chemically functionalized SiO2 surfaces by transmission electron microscopy,” Analytical Chemistry, vol. 78, no. 1, pp. 298–303, 2006.
[8]
A. K. Jha, K. Prasad, and A. R. Kulkarni, “Synthesis of TiO2 nanoparticles using microorganisms,” Colloids and Surfaces B, vol. 71, no. 2, pp. 226–229, 2009.
[9]
P. Mukherjee, A. Ahmad, and A. Ahmad, “Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis,” Nano Letters, vol. 1, no. 10, pp. 515–519, 2001.
[10]
S. Spring and K.-H. Schleifer, “Diversity of magnetotactic bacteria,” Systematic and Applied Microbiology, vol. 18, no. 2, pp. 147–153, 1995.
[11]
D. P. E. Dickson, “Nanostructured magnetism in living systems,” Journal of Magnetism and Magnetic Materials, vol. 203, no. 1–3, pp. 46–49, 1999.
[12]
D. Pum and U. B. Sleytr, “The application of bacterial S-layers in molecular nanotechnology,” Trends in Biotechnology, vol. 17, no. 1, pp. 8–12, 1999.
[13]
R. Joerger, T. Klaus, and C. G. Granqvist, “Biologically produced silver-carbon composite materials for optically functional thin-film coatings,” Advanced Materials, vol. 12, no. 6, pp. 407–409, 2000.
[14]
B. Nair and T. Pradeep, “Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains,” Crystal Growth and Design, vol. 2, no. 4, pp. 293–298, 2002.
[15]
A. Ahmad, S. Senapati, M. I. Khan, R. Kumar, and M. Sastry, “Extracellular biosynthesis of monodisperse gold nanoparticles by a novel extremophilic actinomycete, thermomonospora sp,” Langmuir, vol. 19, no. 8, pp. 3550–3553, 2003.
[16]
C. W. N. Anderson, R. R. Brooks, R. B. Stewart, and R. Simcock, “Harvesting a crop of gold in plants,” Nature, vol. 395, no. 6702, pp. 553–554, 1998.
[17]
J. L. Gardea-Torresdey, E. Gomez, J. R. Peralta-Videa, J. G. Parsons, H. Troiani, and M. Jose-Yacaman, “Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles,” Langmuir, vol. 19, no. 4, pp. 1357–1361, 2003.
[18]
J. Romero-González, J. C. Walton, J. R. Peralta-Videa, E. Rodríguez, J. Romero, and J. L. Gardea-Torresdey, “Modeling the adsorption of Cr(III) from aqueous solution onto Agave lechuguilla biomass: study of the advective and dispersive transport,” Journal of Hazardous Materials, vol. 161, no. 1, pp. 360–365, 2009.
[19]
V. Armendariz, J. G. Parsons, M. L. Lopez, J. R. Peralta-Videa, M. Jose-Yacaman, and J. L. Gardea-Torresdey, “The extraction of gold nanoparticles from oat and wheat biomasses using sodium citrate and cetyltrimethylammonium bromide, studied by x-ray absorption spectroscopy, high-resolution transmission electron microscopy, and UV-visible spectroscopy,” Nanotechnology, vol. 20, no. 10, Article ID 105607, 2009.
[20]
S. P. Chandran, M. Chaudhary, R. Pasricha, A. Ahmad, and M. Sastry, “Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract,” Biotechnology Progress, vol. 22, no. 2, pp. 577–583, 2006.
[21]
S. S. Shankar, A. Ahmad, and M. Sastry, “Geranium leaf assisted biosynthesis of silver nanoparticles,” Biotechnology Progress, vol. 19, no. 6, pp. 1627–1631, 2003.
[22]
M. Sathishkumar, K. Sneha, S. W. Won, C.-W. Cho, S. Kim, and Y.-S. Yun, “Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity,” Colloids and Surfaces B, vol. 73, no. 2, pp. 332–338, 2009.
[23]
P. M. Tessier, O. D. Velev, A. T. Kalambur, J. F. Rabolt, A. M. Lenhoff, and E. W. Kaler, “Assembly of gold nanostructured films templated by colloidal crystals and use in surface-enhanced Raman spectroscopy,” Journal of the American Chemical Society, vol. 122, no. 39, pp. 9554–9555, 2000.
[24]
Y. C. Cao, R. Jin, and C. A. Mirkin, “Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection,” Science, vol. 297, no. 5586, pp. 1536–1540, 2002.
[25]
N. L. Rosi and C. A. Mirkin, “Nanostructures in biodiagnostics,” Chemical Reviews, vol. 105, no. 4, pp. 1547–1562, 2005.
[26]
J. Belloni, “Photography: enhancing sensitivity by silver-halide crystal doping,” Radiation Physics and Chemistry, vol. 67, no. 3-4, pp. 291–296, 2003.
[27]
M. Sanjappa, B. Singh, and M. P. Singh, Botanical Survey of India, 1992.
[28]
A. Vedpriya, “Living Systems: eco-friendly nanofactories,” Digest Journal of Nanomaterials and Biostructures, vol. 5, no. 1, pp. 9–21, 2010.
[29]
O. Collera-Zú?iga, F. García Jiménez, and R. Meléndez Gordillo, “Comparative study of carotenoid composition in three mexican varieties of Capsicum annuum L,” Food Chemistry, vol. 90, no. 1-2, pp. 109–114, 2005.
[30]
B. H. Jagadeesh, T. N. Prabha, and K. Srinivasan, “Improved shelf life of bell capsicum fruits by manipulation of the activities of glycosidases through heat,” Indian Journal of Plant Physiology, vol. 9, no. 2, pp. 164–168, 2004.
[31]
N. Ahmad, M. K. Alam, V. N. Singh, and S. Sharma, “Bioprospecting AgNPs from wild desmodium species,” Journal of Bionanoscience, vol. 3, no. 2, pp. 97–104, 2009.
[32]
V. K. Sharma, R. A. Yngard, and Y. Lin, “Silver nanoparticles: green synthesis and their antimicrobial activities,” Advances in Colloid and Interface Science, vol. 145, no. 1-2, pp. 83–96, 2009.
[33]
J. L. Elechiguerra, J. L. Burt, J. R. Morones, A. Camacho-Bragado, X. Gao, H. H. Lara, and M. J. Yacaman, “Interaction of silver nanoparticles with HIV-1,” Journal of Nanobiotechnology, vol. 3, article no. 6, 2005.
[34]
A. D. Russell and W. B. Hugo, “Antimicrobial activity and action of silver,” Progress in Medicinal Chemistry, vol. 31, pp. 351–370, 1994.
[35]
H. Y. Lee, H. K. Park, Y. M. Lee, K. Kim, and S. B. Park, “Silver nanoparticles and its antibacterial evaluation for biomedical applications,” Chemical Communications, vol. 28, p. 2885, 2007.
[36]
S. H. Jeong, S. Y. Yeo, and S. C. Yi, “The effect of filler particle size on the antibacterial properties of compounded polymer/silver fibers,” Journal of Materials Science, vol. 40, no. 20, pp. 5407–5411, 2005.
[37]
T.-H. Wu, F.-L. Yen, L.-T. Lin, T.-R. Tsai, C.-C. Lin, and T.-M. Cham, “Preparation, physicochemical characterization, and antioxidant effects of quercetin nanoparticles,” International Journal of Pharmaceutics, vol. 346, no. 1-2, pp. 160–168, 2008.
[38]
S. Pal, Y. K. Tak, and J. M. Song, “Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli,” Applied and Environmental Microbiology, vol. 73, no. 6, pp. 1712–1720, 2007.