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冰川冻土  2013 

祁连山高寒草原碱性土壤固氮微生物数量及固氮基因(nifH)群落结构研究

DOI: 10.7522/j.issn.1000-0240.2013.0025, PP. 208-216

Keywords: 祁连山高寒草原,碱性土壤,固氮微生物,固氮基因,群落结构

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Abstract:

对祁连山高寒草原碱性土壤可培养固氮菌数量、固氮基因(nifH)群落结构及其理化性质进行了研究.结果表明固氮菌数量在土层0~40cm处于3.6×105~0.21×105CFUs·g-1之间,除了海拔3001m的土样AQ4外,其他3个土样固氮菌数量随着土壤深度的加深而减小.固氮菌数量与地下生物量呈显著正相关性,与有机碳、可溶有机氮、速效磷和速效钾呈正相关性,而与pH值和全盐呈负相关性.通过基因测序得到的37个固氮基因nifH克隆中,蓝藻门占41%,变形菌门占8%,厚壁菌门占14%,未知菌株占37%;蓝藻门在除了AQ4外的土样中普遍存在,并且是AQ1的优势固氮菌群.此外,还发现了4个新的nifH基因,分别是AQ1-12(KC412109)、AQ4-3(KC412133)、AQ4-4(KC412133)、AQ4-5(KC412133),其中后三者是土样AQ4的优势种群.

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