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冰川冻土  2013 

天山乌鲁木齐河源1号冰川前沿冻土活动层古菌群落的垂直分布格局

DOI: 10.7522/j.issn.1000-0240.2013.0086, PP. 761-769

Keywords: 乌鲁木齐河源1号冰川,冻土活动层,古菌群落,垂直分布,PCR-DGGE

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Abstract:

采用免培养的PCR-DGGE(变性梯度凝胶电泳)指纹、克隆测序、系统发育分析对天山乌鲁木齐河源1号冰川前沿高山草甸冻土活动层古菌群落的垂直分格局进行了研究.结果表明1号冰川前沿高山草甸冻土活动层存在一些优势种群,在所有6个取样深度的土层中均有分布.但不同深度土层古菌群落结构存在明显的差异,在浅表层检测到一些特异的序列,系统发育分析表明它们分别隶属于广古菌门(Euryarchaeota)的盐杆菌纲(Halobacteria)和热原体纲(Thermoplasmata),而在深层土样没有检测到.1号冰川前沿高山草甸带冻土活动层古菌群落以奇古菌(Thaumarchaeota)类群占据绝对优势,全部隶属于被称为group1.1b的谱系.其中,一些序列与不可培养的Nitrososophaera氨氧化古菌序列亲缘关系较近,可能预示着这些中温泉古菌在氨氧化过程中发挥着重要作用,对其群落结构及生态学贡献还有待更深入研究.

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