Neospora caninum (N. caninum), an intracellular protozoan parasite, causes abortion and neonatal mortality in many herbivores including sheep and goats. The aim of this study was to estimate the Seroprevalence of N. caninum in sheep and goats in Grenada, West Indies. Sera were collected from 138 sheep and 138 goats from all six parishes of Grenada and Carriacou, a small island within Grenada. Animals were identified by gender. Sera were tested for antibodies for N. caninum using an indirect Enzyme Linked Immunosorbant Assay (ELISA) multispecies Kit from Pourquire Laboratorie (IDvet, France). Antibodies to N. caninum were detected in 18 of 138 sheep (13%, 95% confidence interval (CI): 7.39% to 18.61%) and 8 of 138 goats (5.8%, 95% CI: 1.9% to 9.7%). Although seropositivity was high in female sheep and in male goats, there was no statistical difference in seropositivity of males and females in both species of animals (p > 0.05, χ2). The results of this study revealed that sheep and goats in Grenada were exposed to N. caninum and dogs might be considered as a source of infection. There was a need to educate the farmers, possessing small ruminants, about the transmission cycle of N. caninum and methods of prevention, to minimize the exposure of their flocks. This is the first report on seroprevalence of N. caninum in Grenada, West Indies.
References
[1]
Dubey, J.P. (2003) Review of Neospora caninum and Neosporosis in Animals. The Korean Journal of Parasitology, 41, 1016. http://dx.doi.org/10.3347/kjp.2003.41.1.1
[2]
Innes, E.A., Lunden, A., Estaban I., Marks, J., Maley, S., Wright, S., Rao, A., Hakins, D., Vermuelen, A., McKendrick, I.J. and Buxton, D. (2001) A Previous Infection with T. gondii Does Not Protect against a Challenge with N. caninum in Pregnant Sheep. Parasite Immunology, 23, 121-132. http://dx.doi.org/10.1046/j.1365-3024.2001.00361.x
[3]
Spilavska, S., Reiterova, K., Kovacova, D., Bobakova, M. and Dubinsky, P. (2009) The First Finding of Neospora caninum and T. gondii and the Occurrence of Other Abortificient Agents in Sheep in Slovakia. Veterinary Parasitology, 164, 320-323. http://dx.doi.org/10.1016/j.vetpar.2009.05.020
[4]
Figliuolo, L.P.C., Kasai, N., Ragozo, A.M.A., de Paula, V.S.O., Dias, R.A., Souza, S.L.P. and Gennari, S.M. (2004) Prevalence of Anti-Toxoplasma gondii and Anti-Neospora caninum Antibodies in Ovine from Sao Paulo State, Brazil. Veterinary Parasitology, 123, 161-166. http://dx.doi.org/10.1016/j.vetpar.2004.06.006
[5]
Herbert, S.S., Silvia, M.M.A., Ana, C.D.S.B., Hilda, F.J.P., Ricardo, A.D. and Solange M.G. (2009) Prevalence of Anti-Toxoplasma gondii and anti-Neospora caninum Antibodies in Sheep from Mossorro, Rio Grande do Norte, Brazil. Veterinary Parasitology, 160, 211-214. http://dx.doi.org/10.1016/j.vetpar.2008.10.102
[6]
Bishop, S., King, J., Windsor, P., Reichel, M.P., Ellis, J. and Slapita, J. (2010) The First Report of Ovine Cerebral Neosporosis and Evaluation of Neospora caninum Prevalence in Sheep in New South Wales. Veterinary Parasitology, 170, 137-142. http://dx.doi.org/10.1016/j.vetpar.2010.01.030
[7]
Michal, C., Jaroslaw, K., Olga, S.J., Mariusz, N., Lucjan, W. and Tadeusz, F. (2011) Seroprevalence of Toxoplasma gondii and Neospora caninum Infections in Goats in Poland. Veterinary Parasitology, 178, 339-341. http://dx.doi.org/10.1016/j.vetpar.2011.01.039
[8]
Uzeda, R.S., Pinheiro, A.M., Fernandez, S.Y., Ayres, M.C.C., Godim, L.F.P. and Almeida, M.A.O. (2007) Seroprevalence of Neospora caninumin Dairy Goats from Brazil. Small Ruminant Research, 70, 257-259. http://dx.doi.org/10.1016/j.smallrumres.2006.04.003
[9]
Anamaria, I., Adriana, G., Viorica, M., Raluca, G. and Vasile, C. (2012) Seroprevalence of Toxoplasma gondii and Neospora caninum in Dairy Goats from Romania. Veterinary Parasitology, 186, 470-474. http://dx.doi.org/10.1016/j.vetpar.2011.11.062
[10]
Langoni, H., Junior, H.G., Guimaraes, F.F., Ulman, L.S., Gairo, F.C., Uehara, R.S., Rosa, E.P., Amorim, R.G., Silva, R.C. (2010) Serological Profile of Toxoplasma gondii and Neosporosis caninum Infection in Commercial Sheep from Sao Paulo State, Brazil. Veterinary Parasitology, 177, 50-54. http://dx.doi.org/10.1016/j.vetpar.2010.11.024
[11]
Hassig, M., Sager, H., Reitt, K., Ziegler, D., Strabel, D. and Gottstein, B. (2003) Neospora caninum in Sheep: A Herd Case Report. Veterinary Parasitology, 117, 213-220. http://dx.doi.org/10.1016/j.vetpar.2003.07.029
[12]
Anastasia, D., Elias, P., Nikolaos, P., Charilaos, K. andektarios, G. (2013) Toxoplasma gondii and Neospora caninum Seroprevalence in Dairy Sheep and Goats Mixed Stock Farming. Veterinary Parasitology, 198, 387-390. http://dx.doi.org/10.1016/j.vetpar.2013.09.017
[13]
Bartova, E., Sedlak, K. and Literak, I. (2009) Toxoplasma gondii and Neospora caninum Antibodies in Sheep in the Czech Republic. Veterinary Parasitology, 161, 131-132. http://dx.doi.org/10.1016/j.vetpar.2008.12.022
[14]
Gaffari, A., Giacometti, M., Tranquillo, V.M., Magnito, S., Cordiolo, P. and Lanfranchi, P. (2006) Serosurvey of Roe Deer, Chamois and Domestic Sheep in Central Italian Alps. Journal of Wildlife Diseases, 42, 685-690. http://dx.doi.org/10.7589/0090-3558-42.3.685
[15]
Faria, E.B., Gennan, S.M., Pena, H.F.J., Cella, A., Maria, R.A., Silva, L.C.R. and Azevedo, S.S. (2007) Prevalence of anti Toxoplasma gondii and Neospora caninum Antibodies in Goats Slaughtered in the Public Slaughterhouses of Patos City, Paraiba State, Northwestern Region of Brazil. Veterinary Parasitology, 149, 126-129. http://dx.doi.org/10.1016/j.vetpar.2007.07.009
[16]
Abo-Shehada, M.N. and Abu-Halaweh, M.M. (2010) Flock Level Seroprevalence of and Risk Factors for Neospora caninum among Sheep and Goats in Northern Jordan. Preventive Veterinary Medicine, 93, 25-32. http://dx.doi.org/10.1016/j.prevetmed.2009.08.004
[17]
Machedo, G.P., Kikuti, M., Langoni, H. and Paes, A.C. (211) Seroprevalence and Risk Factors Associated with Neosporosis in Sheep and Dogs from Farms. Veterinary Parasitology, 182, 356-358.
[18]
Dubey, J.P., Stone, D., Kwok, O.C.H. and Sharma, R.N. (2008) Toxoplasma gondii and Neospora caninum Antibodies in Dogs from Grenada, West Indies. Journal of Parasitology, 94, 750-751. http://dx.doi.org/10.1645/GE-1496.1
[19]
Sharma, R., Theresa, K., Tiwari, K., Alfred Chikweto, A., Thomas, D., Marta Lanza, P. and Muhhamad, I.B. (2015) Serological Evidence of Antibodies to Neospora caninum in Stray and Owned Grenadian Dogs. Tropical Biomedicine, 32, 1-5.
