Lassa virus (LASV) is a causative agent of hemorrhagic fever in West Africa. In recent years, it has been imported several times to Europe and North America. The method of choice for early detection of LASV in blood is RT-PCR. Therefore, the European Network for Diagnostics of ‘Imported’ Viral Diseases (ENIVD) performed an external quality assessment (EQA) study for molecular detection of LASV. A proficiency panel of 13 samples containing various concentrations of inactivated LASV strains Josiah, Lib-1580/121, CSF, or AV was prepared. Samples containing the LASV-related lymphocytic choriomeningitis virus (LCMV) and negative sera were included as specificity controls. Twenty-four laboratories from 17 countries (13 European, one African, one Asian, two American countries) participated in the study. Thirteen laboratories (54%) reported correct results, 4 (17%) laboratories reported 1 to 2 false-negative results, and 7 (29%) laboratories reported 3 to 5 false-negative results. This EQA study indicates that most participating laboratories have a good or acceptable performance in molecular detection of LASV. However, several laboratories need to review and improve their diagnostic procedures.
References
[1]
Frame JD, Baldwin JM Jr., Gocke DJ, Troup JM. Lassa fever, a new virus disease of man from West Africa. I. Clinical description and pathological findings. The American journal of tropical medicine and hygiene. 1970;19(4):670–6. pmid:4246571
[2]
Cummins D, Bennett D, Fisher-Hoch SP, Farrar B, Machin SJ, McCormick JB. Lassa fever encephalopathy: clinical and laboratory findings. The Journal of tropical medicine and hygiene. 1992;95(3):197–201. pmid:1597876
[3]
Emond RT, Bannister B, Lloyd G, Southee TJ, Bowen ET. A case of Lassa fever: clinical and virological findings. British medical journal. 1982;285(6347):1001–2. pmid:6812716 doi: 10.1136/bmj.285.6347.1001
[4]
Murphy FA. Arenavirus taxonomy: a review. Bulletin of the World Health Organization. 1975;52(4–6):389–91. pmid:1085227
[5]
Lecompte E, Fichet-Calvet E, Daffis S, Koulemou K, Sylla O, Kourouma F, et al. Mastomys natalensis and Lassa fever, West Africa. Emerging infectious diseases. 2006;12(12):1971–4. pmid:17326956 doi: 10.3201/eid1212.060812
[6]
Monath TP. Lassa fever: review of epidemiology and epizootiology. Bulletin of the World Health Organization. 1975;52(4–6):577–92. pmid:1085227
[7]
Yalley-Ogunro JE, Frame JD, Hanson AP. Endemic Lassa fever in Liberia. VI. Village serological surveys for evidence of Lassa virus activity in Lofa County, Liberia. Transactions of the Royal Society of Tropical Medicine and Hygiene. 1984;78(6):764–70. pmid:6398530 doi: 10.1016/0035-9203(84)90013-0
[8]
Bowen MD, Peters CJ, Nichol ST. Phylogenetic analysis of the Arenaviridae: patterns of virus evolution and evidence for cospeciation between arenaviruses and their rodent hosts. Molecular phylogenetics and evolution. 1997;8(3):301–16. pmid:9417890 doi: 10.1006/mpev.1997.0436
[9]
Fichet-Calvet E, Rogers DJ. Risk maps of Lassa fever in West Africa. PLoS neglected tropical diseases. 2009;3(3):e388. doi: 10.1371/journal.pntd.0000388. pmid:19255625
[10]
Atkin S, Anaraki S, Gothard P, Walsh A, Brown D, Gopal R, et al. The first case of Lassa fever imported from Mali to the United Kingdom, February 2009. Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin. 2009;14(10).
[11]
Safronetz D, Lopez JE, Sogoba N, Traore SF, Raffel SJ, Fischer ER, et al. Detection of Lassa virus, Mali. Emerging infectious diseases. 2010;16(7):1123–6. doi: 10.3201/eid1607.100146. pmid:20587185
[12]
Akoua-Koffi C, Ter Meulen J, Legros D, Akran V, Aidara M, Nahounou N, et al. [Detection of anti-Lassa antibodies in the Western Forest area of the Ivory Coast]. Medecine tropicale: revue du Corps de sante colonial. 2006;66(5):465–8.
[13]
Gunther S, Lenz O. Lassa virus. Critical reviews in clinical laboratory sciences. 2004;41(4):339–90. pmid:15487592 doi: 10.1080/10408360490497456
[14]
Ajayi NA, Nwigwe CG, Azuogu BN, Onyire BN, Nwonwu EU, Ogbonnaya LU, et al. Containing a Lassa fever epidemic in a resource-limited setting: outbreak description and lessons learned from Abakaliki, Nigeria (January-March 2012). International journal of infectious diseases: IJID: official publication of the International Society for Infectious Diseases. 2013;17(11):e1011–6. doi: 10.1016/j.ijid.2013.05.015. pmid:23871405
Gunther S, Weisner B, Roth A, Grewing T, Asper M, Drosten C, et al. Lassa fever encephalopathy: Lassa virus in cerebrospinal fluid but not in serum. The Journal of infectious diseases. 2001;184(3):345–9. pmid:11443561 doi: 10.1086/322033
[17]
Schmitz H, Kohler B, Laue T, Drosten C, Veldkamp PJ, Gunther S, et al. Monitoring of clinical and laboratory data in two cases of imported Lassa fever. Microbes and infection / Institut Pasteur. 2002;4(1):43–50. doi: 10.1016/s1286-4579(01)01508-8
[18]
Haas WH, Breuer T, Pfaff G, Schmitz H, Kohler P, Asper M, et al. Imported Lassa fever in Germany: surveillance and management of contact persons. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2003;36(10):1254–8. pmid:12746770 doi: 10.1086/374853
[19]
Gunther S, Emmerich P, Laue T, Kuhle O, Asper M, Jung A, et al. Imported lassa fever in Germany: molecular characterization of a new lassa virus strain. Emerging infectious diseases. 2000;6(5):466–76. pmid:10998376 doi: 10.3201/eid0605.000504
[20]
Lassa fever, imported case, Netherlands. Releve epidemiologique hebdomadaire / Section d'hygiene du Secretariat de la Societe des Nations = Weekly epidemiological record / Health Section of the Secretariat of the League of Nations. 2000;75(33):265.
McCormick JB, King IJ, Webb PA, Johnson KM, O'Sullivan R, Smith ES, et al. A case-control study of the clinical diagnosis and course of Lassa fever. The Journal of infectious diseases. 1987;155(3):445–55. pmid:3805772 doi: 10.1093/infdis/155.3.445
[23]
Demby AH, Chamberlain J, Brown DW, Clegg CS. Early diagnosis of Lassa fever by reverse transcription-PCR. Journal of clinical microbiology. 1994;32(12):2898–903. pmid:7883875
[24]
Lunkenheimer K, Hufert FT, Schmitz H. Detection of Lassa virus RNA in specimens from patients with Lassa fever by using the polymerase chain reaction. Journal of clinical microbiology. 1990;28(12):2689–92. pmid:2279999
[25]
Trappier SG, Conaty AL, Farrar BB, Auperin DD, McCormick JB, Fisher-Hoch SP. Evaluation of the polymerase chain reaction for diagnosis of Lassa virus infection. The American journal of tropical medicine and hygiene. 1993;49(2):214–21. pmid:8357084
[26]
Olschlager S, Lelke M, Emmerich P, Panning M, Drosten C, Hass M, et al. Improved detection of Lassa virus by reverse transcription-PCR targeting the 5' region of S RNA. Journal of clinical microbiology. 2010;48(6):2009–13. doi: 10.1128/JCM.02351-09. pmid:20351210
[27]
Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, et al. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. Journal of clinical microbiology. 2002;40(7):2323–30. pmid:12089242 doi: 10.1128/jcm.40.7.2323-2330.2002
[28]
Coulibaly-N'Golo D, Allali B, Kouassi SK, Fichet-Calvet E, Becker-Ziaja B, Rieger T, et al. Novel arenavirus sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Cote d'Ivoire: implications for evolution of arenaviruses in Africa. PloS one. 2011;6(6):e20893. doi: 10.1371/journal.pone.0020893. pmid:21695269
[29]
Vieth S, Drosten C, Lenz O, Vincent M, Omilabu S, Hass M, et al. RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2007;101(12):1253–64. pmid:17905372 doi: 10.1016/j.trstmh.2005.03.018
[30]
Bowen MD, Rollin PE, Ksiazek TG, Hustad HL, Bausch DG, Demby AH, et al. Genetic diversity among Lassa virus strains. Journal of virology. 2000;74(15):6992–7004. pmid:10888638 doi: 10.1128/jvi.74.15.6992-7004.2000
[31]
Niedrig M, Schmitz H, Becker S, Gunther S, ter Meulen J, Meyer H, et al. First international quality assurance study on the rapid detection of viral agents of bioterrorism. Journal of clinical microbiology. 2004;42(4):1753–5. pmid:15071040 doi: 10.1128/jcm.42.4.1753-1755.2004
[32]
Niedrig M, Donoso-Mantke O, Schadler R, members E. The European Network for Diagnostics of Imported Viral Diseases (ENIVD)—12 years of strengthening the laboratory diagnostic capacity in Europe. Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin. 2007;12(4):E070419 5.
[33]
Escadafal C, Olschlager S, Avsic-Zupanc T, Papa A, Vanhomwegen J, Wolfel R, et al. First international external quality assessment of molecular detection of Crimean-Congo hemorrhagic fever virus. PLoS neglected tropical diseases. 2012;6(6):e1706. doi: 10.1371/journal.pntd.0001706. pmid:22745842
[34]
Escadafal C, Paweska JT, Grobbelaar A, le Roux C, Bouloy M, Patel P, et al. International external quality assessment of molecular detection of Rift Valley fever virus. PLoS neglected tropical diseases. 2013;7(5):e2244. doi: 10.1371/journal.pntd.0002244. pmid:23717706
[35]
Domingo C, Niedrig M, Teichmann A, Kaiser M, Rumer L, Jarman RG, et al. 2nd International external quality control assessment for the molecular diagnosis of dengue infections. PLoS neglected tropical diseases. 2010;4(10). doi: 10.1371/journal.pntd.0000833
