A simple method was developed to synthesize the N-succinyl-chitosan (NSC)
successfully. NSC was characterized by FTIR, 1H NMR and element
analysis. The NSC can form nanoparticles with TPP through electrostatic interaction.
The morphology, particle size and zeta potential of the nanoparticle were
investigated and showed a 100 - 200 nm in diameter with a positive potential
9.99 mv. The encapsulation efficiency of
NSC-ES-NPs was 94.76 ± 0.05. The in vitro cell culture indicates that NSC has non-toxicity and cell-compatibility.
NSC-ES-NPs show antitumor activity against MCF-7 cell. Our results demonstrate
that NSC has great potential in the drug controlled release delivery.
References
[1]
Luo, H., Li, J.
and Chen, X. (2010) Antitumor Effect of N-Succinyl-Chitosan
Nanoparticles on K562 Cells. Biomedicine
& Pharmacotherapy, 64, 521-526. http://dx.doi.org/10.1016/j.biopha.2009.09.002
[2]
Park, J.H.,
Saravanakumar, G.,
Kim, K., et al. (2010) Targeted Delivery of Low
Molecular Drugs Using Chitosan and Its Derivatives. Advanced Drug Delivery Reviews, 62, 28-41. http://dx.doi.org/10.1016/j.addr.2009.10.003
[3]
Ying, G.Q.,
Yang, H.,
Yi, Y., et al. (2007) Relationships between the
Molecular Structure and Moisture-Absorption and Moisture-Retention Abilities of
Succinyl Chitosan. Polymer Bulletin, 59,
509-516. http://dx.doi.org/10.1007/s00289-007-0790-9
[4]
Sui, W.,
Wang, Y.,
Dong, S, et al. (2008) Preparation and Properties of an Amphiphilic Derivative of Succinyl- Chito- san. Colloids and Surfaces A: Physicochemical and Engineering Aspects,
316, 171-175. http://dx.doi.org/10.1016/j.colsurfa.2007.09.016
[5]
Zhu, A.P., Chen, T.,
Yuan, L.H.,
Wu, H. and Lu, P. (2006)
Synthesis and Characterization of N-Succinyl-Chitosan and Its Self-Assembly of
Nanospheres. Carbohydrate Polymers, 66,
274-279. http://dx.doi.org/10.1016/j.carbpol.2006.03.014
[6]
Kato, Y.,
Onishi, H. and Machida, Y.
(2001) Biological Characteristics of Lactosaminated N-Succinyl-Chitosan as a Liver-Specific Drug Carrier
in Mice. Journal of Controlled Release,
70, 295-307. http://dx.doi.org/10.1016/S0168-3659(00)00356-4
[7]
Kato, Y., Onishi, H. and Machida, Y. (2001) Lactosaminated and Intact N-Succinyl-Chitosans
as Drug Carriers in Liver
Metastasis. International Journal of Pharmaceutics,
226, 93-106. http://dx.doi.org/10.1016/S0378-5173(01)00777-3
[8]
Asai, K.,
Kanazawa, H.,
Otani, K., et al. (2002) Imbalance between
Vascular Endothelial Growth Factor and Endostatin Levels in Induced Sputum from
Asthmatic Subjects. Journal of Allergy and
Clinical Immunology, 110, 571-575. http://dx.doi.org/10.1067/mai.2002.127797
Takeda, A.,
Shimada, H.,
Imaseki, H., et al. (2000) Clinical Significance of Serum Vascular Endothelial Growth
Factor in Colorectal Cancer Patients: Correlation with Clinicopathological
Factors and Tumor Markers. Oncology Reports,
7, 333-338. http://dx.doi.org/10.3892/or.7.2.333
[11]
Jones A, Fujiyama C,
Turner K, et al. (2000) Elevated Serum
Vascular Endothelial Growth Factor in Patients with Hormone-Escaped Prostate
Cancer. BJU International, 85, 276-280. http://dx.doi.org/10.1046/j.1464-410x.2000.00432.x
[12]
Suzuki, M.,
Iizasa, T., Ko, E., et al. (2002) Serum Endostatin
Correlates with Progression and Prognosis of Non-Small Cell Lung Cancer. Lung Cancer, 35, 29-34. http://dx.doi.org/10.1016/S0169-5002(01)00285-9
[13]
Folkman, J. (2006) Antiangiogenesis in Cancer Therapy—Endostatin and Its Mechanisms of Action. Experimental Cell Research, 312, 594-607. http://dx.doi.org/10.1016/j.yexcr.2005.11.015
[14]
O’Reilly, M.S.,
Boehm, T.,
Shing, Y., et al. (1997) Endostatin: An Endogenous Inhibitor of Angiogenesis and Tumor
Growth. Cell, 88, 277-285. http://dx.doi.org/10.1016/S0092-8674(00)81848-6
[15]
Nilsson, U.W. and Dabrosin, C. (2006)
Estradiol and Tamoxifen Regulate Endostatin Generation via Matrix Metalloproteinase
Activity in Breast Cancer in Vivo. Cancer Research, 66, 4789-4794. http://dx.doi.org/10.1158/0008-5472.CAN-05-4012
Hajitou, A.,
Grignet, C.,
Devy, L., et al. (2002) The Antitumoral Effect of
Endostatin and Angiostatin Is Associated with a Down-Regulation of Vascular
Endothelial Growth Factor Expression in Tumor Cells. The FASEB Journal, 16, 1802-1804. http://dx.doi.org/10.1096/fj.02-0109fje
