The research of degree of incidence of genomic changes (of micronuclei) in mucous cells of gastric superficial-foveolar epithelium in contaminated and not contaminated mucous of stomach was made. Histopathological research of micronuclei and helicobacter was made in gastric biopsy specimen of patients with diagnosis-chronical gastritis (ICD-10K29.3) in group of patients who lived in radiation contaminated areas and in groups of people, who had no factors of radiation influence in anamnesis. People, who lived in the radiation contaminated areas, whose mucous was infected by Helicobacter pylori, had the highest frequency of mucous cells with micronuclei. In the group of patients from radiation contaminated areas with HP-associated gastritis frequency of appearance of mucous cells with micronucleus in the mucous of stomach have five time more, than patients, whose mucous of stomach was not infected (p < 0.001). Therefore, we can suggest, that Helicobacter pylori can success in mutagenic effect of radiation factor.
References
[1]
Kohler, A. and Ellesat, K. (2008) Nuclear Changes in Blood, Early Liver Anomalies and Hepatocellular Cancers in Flounder (Platichthysflesus L.) as Prognostic Indicator for a Higher Cancer Risk? Marine Environmental Research, 66, 50-149. http://dx.doi.org/10.1016/j.marenvres.2008.02.071
[2]
Zhang, Z. and Farthing, M. (2000) Helicobacter pylori in Gastric Malignancy: Role of Oxidants, Antioxidants and Other Co-Factors/Helicobacter pylori. In: Hunt, R. and Tytgat, G., Eds., Basic Mechanisms to Clinical Cure, Kluwer Academic Publishers, Dordrecht, 689 p.
[3]
Ohyama, W., Okada, E., Fujiishi, Y., Narumi, K. and Yasutake, N. (2013) In Vivo Rat Glandular Stomach and Colon Micronucleus Tests: Kinetics of Micro-nucleated Cells, Apoptosis, and Cell Proliferation in the Target Tissues after a Single Oral Administration of Stomach- or Colon-Carcinogens. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 755, 141-147. http://dx.doi.org/10.1016/j.mrgentox.2013.06.016
[4]
Kravtsov, V.Y. and Kitaeva, L.V. (2011) Helicobacter pylori Infection and Mucocytes with Micronuclei in the Gastric Mucosa from the Liquidator. Medical-Biological and Social-Psychological Issues of Safety in Emergency Situations, 2, 84-88.
[5]
Kitaeva, L.V., Mikhailova, I.A., Semov, D.M., Proshin, S.N. and Kravtsov, V.Y. (2008) Micronuclei in Mucose Cells and Colonization of Human Stomach Epithelium with Coccoid Forms of Helicobacter pylori. Tsitologiia, 2, 160-164.
[6]
Speit, G., Schütz, P., Weber, I., Ma-Hock, L., Kaufmann, W., Gelbke, H.P. and Durrer, S. (2011) Analysis of Micronuclei, Histopathological Changes and Cell Proliferation in Nasal Epithelium Cells of Rats after Exposure to Formaldehyde by Inhalation. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 721, 35-127. http://dx.doi.org/10.1016/j.mrgentox.2011.01.008
[7]
Lisochkin, B.G., Kravtsov, V.Y. and Rybachenko, V.V. (2004) Retrospective Diagnosis of Radiation Inhalation Lesions of the Bronchial Mucosa in Clean-Up Personnel after the Nuclear Accident at the Chernobyl Nuclear Reactor Using the Micronucleus Test. Archives of Pathology, 2, 17-19.
[8]
Caruso, R.A., Fedele, F., Crisafulli, C., Paparo, D., Parisi, A., Lucianò, R. and Cavallari, V. (2011) Abnormal Nuclear Structures (Micronuclei, Nuclear Blebs, Strings, and Pockets) in a Case of Anaplastic Giant Cell Carcinoma of the Thyroid: An Immunohistochemical and Ultrastructural Study. Ultrastructural Pathology, 35, 8-14. http://dx.doi.org/10.3109/01913123.2010.517899
[9]
Chitcholtan, K., Hampton, M.B. and Keenan, J.I. (2008) Outer Membrane Vesicles Enhance the Carcinogenic Potential of Helicobacter pylori. Carcinogenesis, 29, 5-12. http://dx.doi.org/10.1093/carcin/bgn218
[10]
Arabski, M., Klupinska, G., Chojnacki, J., Kazmierczak, P., Wisniew-ska-Jarosinska, M., Drzewoski, J. and Blasiak, J. (2005) DNA Damage and Repair in Helicobacter pylori-Infected Gastric Mucosa Cells. Mutation Research—Genetic Toxicology and Environmental Mutagenesis, 570, 129-135. http://dx.doi.org/10.1016/j.mrfmmm.2004.10.006
[11]
Guidi, R., Guerra, L., Levi, L., Stenerlow, B., Fox, J.G., Josen-hans, C., Masucci, M.G. and Frisan, T. (2013) Chronic Exposure to the Cytolethal Distending Toxins of Gram-Negative Bacteria Promotes Genomic Instability and Altered DNA Damage Response. Cellular Microbiology, 15, 98-113. http://dx.doi.org/10.1111/cmi.12034
