Pterocarpus osun plant has been used overtime in the indigenous system of medicine. In this study, methanol, ethanol and aqueous extracts of leaves of P. osun were examined against five Escherichia coli strains obtained from wounds. Antibacterial effect of the plant leaves, in the concentration of 100 mg/ml, was determined using disc diffusion method. Phytochemical screening of extracts of the leaves revealed the presence of phenols, flavonoids, tannins, terpenoids alkaloids, and glycosides. Leaves extracts of P. osun showed different zones of inhibition to the Escherichia coli isolates in the different solvents used. Results from this study showed that extracts of P.osun had broad spectrum of activity on the tested bacteria.
References
[1]
Hutchinson, J., Dalziel,
J.M. and Keay, R.W.J. (1958) Floral of West Tropical Africa. Agents for
Overseas Government and Administration, London, 531.
[2]
Rao, S.P. and Raju,
A.J.S. (2002) Pollination Ecology of the Red Sanders Pterocarpussantalinus (Fabaceae), an Endemic and
Endangered tree Species. Current Science India, 83, 1144-1148.
[3]
Okigbo, R.N., Eme,
U.E. and Ogbogu, S. (2008) Biodiversity and Conservation of Medicinal and Aromatic Plants in
Africa. Biotechnology and Molecular
Biology Reviews, 3, 127-134.
Mukherjee, P.K., Maiti, K., Mukherjee,
K. and Houghton, P. (2006) Leads from Indian Medicinal Plants with Hypoglycemic Potentials. Journal of Ethnopharmacology, 106, 1-28. http://dx.doi.org/10.1016/j.jep.2006.03.021
[6]
Krishnaveni,
K.S. and Rao, J.V. (2000) A New Isoflavoneglucoside from Pterocarpussantalinus. Journal of Asian Natural products Research, 2, 219-223.
[7]
Manjunatha, B.K. (2006) Antimicrobial
Activity of Pterocarpussantalinus. Indian Journal of Parmaceutical Sciences, 68, 115-116. http://dx.doi.org/10.4103/0250-474X.22982
[8]
Narayan, S., Devi, R.S.
and Devi, C.S.S. (2007) Role of Pterocarpussantalinus against Mitochondrial Dysfunction and Membrane Lipid Changes
Induced by Ulcerogens in Rat Gastric Mucosa. Chemico-Biological Interactions, 170, 67-75. http://dx.doi.org/10.1016/j.cbi.2007.07.005
[9]
Akingbade, O.A., Balogun,
S.A., Ojo, D.A., Afolabi, R.O., Motayo, B.O., Okerentugba, P.O. and Okonko, I.O. (2012) Plasmid
Profile Analysis of Multidrug Resistant Pseudomonas
aeruginosa isolated from Wound Infections in South West Nigeria. World Applied Science Journal, 20, 766-775.
[10]
Nwachukwu, N.C., Orji,
F.A. and Okike, U.M. (2009) Antibiotic Susceptibility Patterns of Bacterial Isolates from Surgical
Wounds in Abia State University Teaching Hospital (ABSUTH), Aba-Nigeria. Research Journal of Medicine and Medical
Sciences, 4, 575-579.
[11]
Ayton,
M. (1985) Wound Care: Wounds That Won’t Heal. Nursing Times, 81, 16-19.
[12]
Magiorakos, A.P., Srinivasan,
A., Carey, R.B.,Carmeli,
Y., Falagas, M.E., Giske, C.G., et al. (2011) Multidrug-Resistant,
Extensively Drug-Resistant and Pandrug-Resistant Bacteria: An International
Expert Proposal for Interim Standard Definitions for Acquired Resistance. Clinical Microbiology and Infectious
Diseases, 18, 268-281. http://dx.doi.org/10.1111/j.1469-0691.2011.03570.x
[13]
Bartoloni,
A., Pallecchi, L., Benedetti, M.,Fernandez,
C., Vallejos, Y., Guzman, E., et al. (2006) Multidrug-Resistant Commensal Escherichia
coli in Children, Peru and Bolivia. Emerging Infectious Diseases, 12, 907-913. http://dx.doi.org/10.3201/eid1206.051258
[14]
von Baum, H. and Reinhard, M. (2005) Antimicrobial Resistance of Escherichia
coli and Therapeutic Implications. International
Journal of Medical Microbiology, 295, 503-511. http://dx.doi.org/10.1016/j.ijmm.2005.07.002
[15]
Aibinu,
I., Adenipekun, E. and Odugbemi, T. (2004) Emergence of Quinolone Resistance amongst Escherichia coli Strains Isolated from Clinical Infections in Some
Lagos State Hospitals in Nigeria. Nigeria Journal Health
Biomedical Science, 3, 73-78. http://dx.doi.org/10.4314/njhbs.v3i2.11513
[16]
Celebi,
A., Duran, N., Ozturk, F., Acik, L., Aslan, G. and Aslantas, O. (2007) Identification of Clinic Uropathogen Escherichia coli Isolates by Antibiotic Susceptibility, Plasmid and
Whole Cell Protein Profiles. Advance Molecular
Biology, 1, 31-40.
[17]
Kent, P.N., Dixon, D.M., Holland, S.M.
and Fauci, A.S. (2008) The Research Agenda of the National Institute of Allergy and
Infectious Diseases for Antimicrobial Resistance. The Journal of Infectious Diseases, 197, 1087-1093. http://dx.doi.org/10.1086/533451
[18]
Talukdar,
A.D., Choudhary, M.D., Chakraborty, M. and Dutta, B.K. (2010) Phytochemical
Screening and TLC Profiling of Plant Extracts Cyatheagigantea and Cyatheabrunoniana. Biological and Environmental Sciences, 5, 70-74.
[19]
Akueshi,
C.O., Kadiri, C.O., Akueshi, E.U., Agina, S.E. and Njurukwem, C. (2002) Antimicrobial Potentials
of HyptissuaveolensPoit (Lamiaceae). Nigerian Journal of Botany, 15, 37-41.
Farooq,
A., Sajid, L., Muhammad, A. and Anwarul, H.G. (2007) Moringaoleifera: A Food Plant with Multiple Medicinal Uses. Phytotherapy Research, 21, 17-25. http://dx.doi.org/10.1002/ptr.2023
[22]
Burjaq, S.Z. and Shehabi,
A.A. (2013) Fresh Leafy Green Vegetables Associated with Multidrug Resistant E.coli. The International Arabic Journal of
Antimicrobial Agents, 3, 3.
[23]
Akingbade,
O.A., Damola, A.B., Shobayo, B.I., Nwanze, J.C. and Okonko, I.O. (2014) Multi-Drug
Resistant (MDR) Escherichia coli among Children Suffering from Diarrhea Infections in Abeokuta, Nigeria. Researcher, 6, 11-17.
[24]
Gabriel, A.F. and Onigbanjo,
H.O. (2010) Phytochemical and Antimicrobial Screening of the Stem Bark Extracts
of Pterocarpuserinaceus (Poir). Nigerian Journal of
Basic and Applied Sciences, 18, 1-5. http://dx.doi.org/10.4314/njbas.v18i1.56835
[25]
Sharma,
N., Kachhawa, J.B.S., Tyagi, S., Gupta, R.S. and Sharma, K.K. (2012) In Vitro Evaluation of
Antibacterial Activity of PterocarpusmarsupiumRoxb. International Journal of
Pharmacy and Pharmaceutical Sciences, 4, 67-68.
[26]
Zee-Cheng,
R.K. (1997) Anticancer Research on Loranthaceae Plants. Drugs Future, 22, 519-530.
[27]
Tsuchiya, H., Sato, M., Miyazaki, T., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T.
and Linuma, M. (1996) Comparative Study on the Antibacterial Activity of Phytochemical
Flavanones against Methicillin-Resistant Staphylococcus
aureus. Journal of Ethnopharmarcology, 50, 27-34. http://dx.doi.org/10.1016/0378-8741(96)85514-0
