Prevalence of Aflatoxin B1 and B2 in Poultary Feed

A total of 65 poultry feed samples were examined for the detection of aflatoxin (aflatoxin B 1 and aflatoxin B 2 ) using thin layer chromatography (TLC). Samples were collected from Chitwan and Kavrepalanchock districts. Out of those samples examined a total of 49 (75.38%) samples were found positive. Out of 49 (75.38%) samples positive, 42 (85.71%) samples were found positive both with aflatoxin B 1 and B 2 where as five (10.20%) samples were positive only with aflatoxin B1 and two (4.08%) samples were positive only with aflatoxin B2. Among them 13 (20%) samples were found positive having aflatoxin above permissible level. The concentration of aflatoxin in positive samples ranged from trace to 366 ppb (366 μg/kg). Likewise, out of 52 samples examined in rainy season, 40 samples (76.92%) were found positive where as out of 13 samples examined in winter season 9 (69.23%) were found positive. Nepal Agric. Res. J. Vol. 9, 2009, pp. 109-112 DOI: http://dx.doi.org/10.3126/narj.v9i0.11648