Cleanup of herbicide-contaminated soils has been a dire environmental concern since the advent of industrial era. Although microorganisms are excellent degraders of herbicide compounds in the soil, some reparation may need to be brought about, in order to stimulate them to degrade the herbicide at a faster rate in a confined time frame. “Biostimulation” through the appropriate utilization of organic amendments and nutrients can accelerate the degradation of herbicides in the soil. However, effective use of biostimulants requires thorough comprehension of the global redox cycle during the microbial degradation of the herbicide molecules in the soil. In this paper, we present the prospects of using biostimulation as a powerful remediation strategy for the rapid cleanup of herbicide-polluted soils. 1. Introduction The term “biostimulation” is often used to describe the addition of electron acceptors, electron donors, or nutrients to stimulate naturally occurring microbial populations [1]. Comprehensively, biostimulation could be perceived as including the introduction of adequate amounts of water, nutrients, and oxygen into the soil, in order to enhance the activity of indigenous microbial degraders [2] or to promote cometabolism [3]. “Biostimulation” is usually paired under the “enhanced bioremediation” techniques along with “bioaugmentation” which is merely the introduction of specific microorganisms (indigenous or nonindigenous) aimed at enhancing the biodegradation of target compound or serving as donors of the catabolic genes. The concept of biostimulation is to boost the intrinsic degradation potential of a polluted matrix through the accumulation of amendments, nutrients, or other limiting factors and has been used for a wide variety of xenobiotics [4]. Even though the diversity of natural microbial populations apparently displays the potential for contaminant remediation at polluted sites, factors such as lack of electron acceptors or donors, low nitrogen or phosphorus availability, or a lack of stimulation of the metabolic pathways responsible for degradation can inhibit or delay the remediation. In these cases, accumulation of exogenous nutrients can enhance the degradation of the toxic materials [5]. Herbicides are a group of compounds that, in spite of their benefits, may produce a wide range of toxic side effects which pose a potential threat to the environment. Extensive use of herbicides poses some far-reaching consequence because of the potential runoff and leaching of these compounds through the soil leading to contamination of surface and
References
[1]
K. M. Scow and K. A. Hicks, “Natural attenuation and enhanced bioremediation of organic contaminants in groundwater,” Current Opinion in Biotechnology, vol. 16, no. 3, pp. 246–253, 2005.
[2]
M. N. P. F. S. Couto, E. Monteiro, and M. T. S. D. Vasconcelos, “Mesocosm trials of bioremediation of contaminated soil of a petroleum refinery: comparison of natural attenuation, biostimulation and bioaugmentation,” Environmental Science and Pollution Research, vol. 17, no. 7, pp. 1339–1346, 2010.
[3]
V. de Lorenzo, “Systems biology approaches to bioremediation,” Current Opinion in Biotechnology, vol. 19, no. 6, pp. 579–589, 2008.
[4]
N. Kadian, A. Gupta, S. Satya, R. K. Mehta, and A. Malik, “Biodegradation of herbicide (atrazine) in contaminated soil using various bioprocessed materials,” Bioresource Technology, vol. 99, no. 11, pp. 4642–4647, 2008.
[5]
L. Cosgrove, P. L. McGeechan, P. S. Handley, and G. D. Robson, “Effect of biostimulation and bioaugmentation on degradation of polyurethane buried in soil,” Applied and Environmental Microbiology, vol. 76, no. 3, pp. 810–819, 2010.
[6]
M. Graymore, F. Stagnitti, and G. Allinson, “Impacts of atrazine in aquatic ecosystems,” Environment International, vol. 26, no. 7-8, pp. 483–495, 2001.
[7]
I. Buchanan, H. C. Liang, W. Khan et al., “Pesticides and herbicides,” Water Environment Research, vol. 81, no. 10, pp. 1731–1816, 2009.
[8]
J. M. Bollag and S. Y. Liu, “Biological transformation processes of pesticides,” in Pesticides in the Soil Environment: Processes, Impacts, and Modeling, pp. 169–211, Soil Science Society of America, Madison, Wis, USA, 1990.
[9]
D. W. Kolpin, E. M. Thurman, and S. M. Linhart, “The environmental occurrence of herbicides: the importance of degradates in ground water,” Archives of Environmental Contamination and Toxicology, vol. 35, no. 3, pp. 385–390, 1998.
[10]
G. K. Sims and A. M. Cupples, “Factors controlling degradation of pesticides in soil,” Pesticide Science, vol. 55, no. 5, pp. 598–601, 1999.
[11]
Y. Qiu, H. Pang, Z. Zhou, P. Zhang, Y. Feng, and G. D. Sheng, “Competitive biodegradation of dichlobenil and atrazine coexisting in soil amended with a char and citrate,” Environmental Pollution, vol. 157, no. 11, pp. 2964–2969, 2009.
[12]
M. Dua, A. Singh, N. Sethunathan, and A. Johri, “Biotechnology and bioremediation: successes and limitations,” Applied Microbiology and Biotechnology, vol. 59, no. 2-3, pp. 143–152, 2002.
[13]
D. K. Singh, “Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments,” Indian Journal of Microbiology, vol. 48, no. 1, pp. 35–40, 2008.
[14]
N. Sethunathan, K. Raghu, H. C. Aggarwal, and R. Naidu, “Biremediation of pesticides in soils through enhancement of Indigenous microbial activities,” in Proceedings of the 2nd International Conference on Contaminants in Soil Environment in Australia-Pacific Region, 1999..
[15]
S. El Fantroussi and S. N. Agathos, “Is bioaugmentation a feasible strategy for pollutant removal and site remediation?” Current Opinion in Microbiology, vol. 8, no. 3, pp. 268–275, 2005.
[16]
M. Alexander, “Acclimation,” in Biodegradation and Bioremediation, pp. 16–40, Academic Press, San Diego, Calif, USA, 1994.
[17]
E. Silva, A. M. Fialho, I. Sa-Correia, R. G. Burns, and L. J. Shaw, “Combined bioaugmentation and biostimulation to cleanup soil contaminated with high concentrations of atrazine,” Environmental Science & Technology, vol. 38, no. 2, pp. 632–637, 2004.
[18]
T. F. Guerin, “Ex-situ bioremediation of chlorobenzenes in soil,” Journal of Hazardous Materials, vol. 154, no. 1-3, pp. 9–20, 2008.
[19]
G. K. Sims, J. D. Wolt, and R. G. Lehmann, “Bioavailability of sorbed pesticides and other xenobiotic molecules,” in Proceedings of the International Symposium on Environmental Aspects of Pesticide Microbiology, pp. 159–164, Sigtuna, Sweden, August 1992.
[20]
G. K. Sims, S. Taylor-Lovell, G. Tarr, and S. Maskel, “Role of sorption and degradation in the herbicidal function of isoxaflutole,” Pest Management Science, vol. 65, no. 7, pp. 805–810, 2009.
[21]
T. A. Johnson and G. K. Sims, “Introduction of 2,4-dichlorophenoxyacetic acid into soil with solvents and resulting implications for bioavailability to microorganisms,” World Journal of Microbiology and Biotechnology, pp. 1–7, 2010.
[22]
N. Kosaric, “Biosurfactants and their application for soil bioremediation,” Food Technology and Biotechnology, vol. 39, no. 4, pp. 295–304, 2001.
[23]
J. C. Mata-Sandoval, J. Karns, and A. Torrents, “Effect of rhamnolipids produced by Pseudomonas aeruginosa UG2 on the solubilization of pesticides,” Environmental Science & Technology, vol. 34, no. 23, pp. 4923–4930, 2000.
[24]
J. C. Mata-Sandoval, J. Karns, and A. Torrents, “Influence of rhamnolipids and triton X-100 on the biodegradation of three pesticides in aqueous phase and soil slurries,” Journal of Agricultural and Food Chemistry, vol. 49, no. 7, pp. 3296–3303, 2001.
[25]
J. M. Bollag, J. Dec, and P. M. Huang, “Formation mechanisms of complex organic structures in soil habitats,” Advances in Agronomy, vol. 63, pp. 237–266, 1997.
[26]
B. Gevao, K. T. Semple, and K. C. Jones, “Bound pesticide residues in soils: a review,” Environmental Pollution, vol. 108, no. 1, pp. 3–14, 2000.
[27]
J. E. Kim, E. Fernandes, and J. M. Bollag, “Enzymatic coupling of the herbicide bentazon with humus monomers and characterization of reaction products,” Environmental Science & Technology, vol. 31, no. 8, pp. 2392–2398, 1997.
[28]
J. M. Bollag, “Decontamination of soils through immobilization of anthropogenic organics by biotic and abiotic catalysts,” in Molecular Environmental Soil Science at the Interfaces in the Earth's Critical Zone, J. Xu and P. M. Huang, Eds., pp. 182–183, Springer, Berlin, Germany, 2010.
[29]
A. S. Felsot and E. K. Dzantor, “Effect of alachlor concentration and an organic amendment on soil dehydrogenase activity and pesticide degradation rate,” Environmental Toxicology and Chemistry, vol. 14, no. 1, pp. 23–28, 1995.
[30]
J. A. Pascual, C. García, T. Hernandez, and M. Ayuso, “Changes in the microbial activity of an arid soil amended with urban organic wastes,” Biology and Fertility of Soils, vol. 24, no. 4, pp. 429–434, 1997.
[31]
M. Tejada, A. M. García-Martínez, I. Gómez, and J. Parrado, “Application of MCPA herbicide on soils amended with biostimulants: short-time effects on soil biological properties,” Chemosphere, vol. 80, no. 9, pp. 1088–1094, 2010.
[32]
A. S. Felsot and E. K. Dzantor, “Enhancing biodegradation for detoxification of herbicide waste in soil,” ACS Symposium Series, vol. 426, pp. 249–268, 1990.
[33]
C. G. Cogger, P. R. Bristow, J. D. Stark, L. W. Getzin, and M. Montgomery, “Transport persistence of pesticides in alluvial soils: I. Simazine,” Journal of Environmental Quality, vol. 27, no. 3, pp. 543–550, 1998.
[34]
F. Büyüks?nmez, R. Rynk, T. F. Hess, and E. Bechinski, “Occurrence, degradation and fate of pesticides during composting part I: composting, pesticides, and pesticide degradation,” Compost Science & Utilization, vol. 7, no. 4, pp. 66–82, 1999.
[35]
M. Vidali, “Bioremediation. An overview,” Pure and Applied Chemistry, vol. 73, no. 7, pp. 1163–1172, 2001.
[36]
R. J. Hance, “The effect of nutrients on the decomposition of the herbicides atrazine and linuron incubated with soil,” Pesticide Science, vol. 4, no. 6, pp. 817–822, 1973.
[37]
F. Bichat, G. K. Sims, and R. L. Mulvaney, “Microbial utilization of heterocyclic nitrogen from atrazine,” Soil Science Society of America Journal, vol. 63, no. 1, pp. 100–110, 1999.
[38]
G. K. Sims, “Nitrogen starvation promotes biodegradation of N-heterocyclic compounds in soil,” Soil Biology & Biochemistry, vol. 38, no. 8, pp. 2478–2480, 2006.
[39]
L. Guo, T. J. Bicki, A. S. Felsot, and T. D. Hinesly, “Phytotoxicity of atrazine and alachlor in soil amended with sludge, manure and activated carbon,” Journal of Environmental Science and Health, Part B, vol. 26, no. 5-6, pp. 513–527, 1991.
[40]
V. Leoni, C. Cremisini, R. Giovinazzo, G. Puccetti, and M. Vitali, “Activated sludge biodegradation test as a screening method to evaluate persistence of pesticides in soil,” Science of the Total Environment, vol. 123-124, pp. 279–289, 1992.
[41]
E. K. Dzantor, A. S. Felsot, and M. J. Beck, “Bioremediating herbicide-contaminated soils,” Applied Biochemistry and Biotechnology, vol. 39-40, no. 1, pp. 621–630, 1993.
[42]
H. Printz, P. Burauel, and F. Fuhr, “Effect of organic amendment on degradation and formation of bound residues of methabenzthiazuron in soil under constant climatic conditions,” Journal of Environmental Science and Health, Part B, vol. 30, no. 4, pp. 435–456, 1995.
[43]
J. Gan, R. L. Becker, W. C. Koskinen, and D. D. Buhler, “Degradation of atrazine in two soils as a function of concentration,” Journal of Environmental Quality, vol. 25, no. 5, pp. 1064–1072, 1996.
[44]
E. Topp, L. Tessier, and E. G. Gregorich, “Dairy manure incorporation stimulates rapid atrazine mineralization in an agricultural soil,” Canadian Journal of Soil Science, vol. 76, no. 3, pp. 403–409, 1996.
[45]
S. C. Wagner and R. M. Zablotowicz, “Effect of organic amendments on the bioremediation of cyanazine and fluometuron in soil,” Journal of Environmental Science and Health, Part B, vol. 32, no. 1, pp. 37–54, 1997.
[46]
A. S. Felsot and E. K. Dzantor, “Potential of biostimulation to enhance dissipation of aged herbicide residues in land-farmed waste,” in Phytoremediation of Soil and Water Contaminants, E. L. Kruger, T. A. Anderson, and J. R. Coats, Eds., pp. 77–91, 1997.
[47]
R. Abdelhafid, S. Houot, and E. Barriuso, “Dependence of atrazine degradation on C and N availability in adapted and non-adapted soils,” Soil Biology & Biochemistry, vol. 32, no. 3, pp. 389–401, 2000.
[48]
T. B. Moorman, J. K. Cowan, E. L. Arthur, and J. R. Coats, “Organic amendments to enhance herbicide biodegradation in contaminated soils,” Biology and Fertility of Soils, vol. 33, no. 6, pp. 541–545, 2001.
[49]
L. Delgado-Moreno and A. Pe?a, “Organic amendments from olive cake as a strategy to modify the degradation of sulfonylurea herbicides in soil,” Journal of Agricultural and Food Chemistry, vol. 55, no. 15, pp. 6213–6218, 2007.
[50]
I. Mukherjee, “Effect of organic amendments on degradation of atrazine,” Bulletin of Environmental Contamination and Toxicology, vol. 83, no. 6, pp. 832–835, 2009.
[51]
J. R. de Lipthay, S. R. S?rensen, and J. Aamand, “Effect of herbicide concentration and organic and inorganic nutrient amendment on the mineralization of mecoprop, 2,4-D and 2,4,5-T in soil and aquifer samples,” Environmental Pollution, vol. 148, no. 1, pp. 83–93, 2007.
[52]
E. El-Bestawy and H. J. Albrechtsen, “Effect of nutrient amendments and sterilization on mineralization and/or biodegradation of 14C-labeled MCPP by soil bacteria under aerobic conditions,” International Biodeterioration & Biodegradation, vol. 59, no. 3, pp. 193–201, 2007.
[53]
I. Harrison, G. M. Williams, and C. A. Carlick, “Enantioselective biodegradation of mecoprop in aerobic and anaerobic microcosms,” Chemosphere, vol. 53, no. 5, pp. 539–549, 2003.
[54]
C. Perrin-Ganier, F. Schiavon, J. L. Morel, and M. Schiavon, “Effect of sludge-amendment or nutrient addition on the biodegradation of the herbicide isoproturon in soil,” Chemosphere, vol. 44, no. 4, pp. 887–892, 2001.
[55]
G. M. Wolfaardt, J. R. Lawrence, R. D. Robarts, and D. E. Caldwell, “The role of interactions, sessile growth, and nutrient amendments on the degradative efficiency of a microbial consortium,” Canadian Journal of Microbiology, vol. 40, no. 5, pp. 331–340, 1994.
[56]
N. A. Assaf and R. F. Turco, “Influence of carbon and nitrogen application on the mineralization of atrazine and its metabolites in soil,” Pesticide Science, vol. 41, no. 1, pp. 41–47, 1994.
[57]
R. Abdelhafid, S. Houot, and E. Barriuso, “How increasing availabilities of carbon and nitrogen affect atrazine behaviour in soils,” Biology and Fertility of Soils, vol. 30, no. 4, pp. 333–340, 2000.
[58]
L. L. McCormick and A. E. Hiltbold, “Microbiological decomposition of atrazine and diuron is soil,” Weeds, vol. 14, no. 1, pp. 77–82, 1966.
[59]
G. H. Wagner and K. S. Chahal, “Decomposition of carbon-14 labelled atrazine in soil samples from Sanborn field1,” Soil Science Society of America Journal, vol. 30, no. 6, pp. 752–754, 1996.
[60]
G. A. Barrio-Lage, F. Z. Parsons, R. M. Narbaitz, and P. A. Lorenzo, “Enhanced anaerobic biodegration of vinyl chloride in ground water,” Environmental Toxicology and Chemistry, vol. 9, no. 4, pp. 403–415, 1990.
[61]
E. Topp, R. L. Crawford, and R. S. Hanson, “Influence of readily metabolizable carbon on pentachlorophenol metabolism by a pentachlorophenol-degrading Flavobacterium sp,” Applied and Environmental Microbiology, vol. 54, no. 10, pp. 2452–2459, 1988.
[62]
A. P. Ensz, C. W. Knapp, and D. W. Graham, “Influence of autochthonous dissolved organic carbon and nutrient limitation on alachlor biotransformation in aerobic aquatic systems,” Environmental Science & Technology, vol. 37, no. 18, pp. 4157–4162, 2003.
[63]
C. W. Knapp, D. W. Graham, G. Berardesco, F. DeNoyelles, B. J. Cutak, and C. K. Larive, “Nutrient level, microbial activity, and alachlor transformation in aerobic aquatic systems,” Water Research, vol. 37, no. 19, pp. 4761–4769, 2003.
[64]
J. P. M. Vink, G. Schraa, and S. E. A. T. M. van der Zee, “Nutrient effects on microbial transformation of pesticides in nitrifying surface waters,” Environmental Toxicology, vol. 14, no. 3, pp. 329–338, 1999.
[65]
H. J. Nesbitt and J. R. Watson, “Degradation of the herbicide 2,4-D in river water. II. The role of suspended sediment, nutrients and water temperature,” Water Research, vol. 14, no. 12, pp. 1689–1694, 1980.
[66]
J. M. Tor, C. Xu, J. M. Stucki, M. M. Wander, and G. K. Sims, “Trifluralin degradation under microbiologically induced nitrate and Fe(III) reducing conditions,” Environmental Science & Technology, vol. 34, no. 15, pp. 3148–3152, 2000.
[67]
J. J. Crawford, G. K. Sims, R. L. Mulvaney, and M. Radosevich, “Biodegradation of atrazine under denitrifying conditions,” Applied Microbiology and Biotechnology, vol. 49, no. 5, pp. 618–623, 1998.
[68]
I. Katz, C. G. Dosoretz, R. T. Mandelbaum, and M. Green, “Atrazine degradation under denitrifying conditions in continuous culture of Pseudomonas ADP,” Water Research, vol. 35, no. 13, pp. 3272–3275, 2001.
[69]
L. Larsen and J. Aamand, “Degradation of herbicides in two sandy aquifers under different redox conditions,” Chemosphere, vol. 44, no. 2, pp. 231–236, 2001.
[70]
N. Shapir, R. T. Mandelbaum, and G. S. Jacobsen, “Rapid atrazine mineralization under denitrifying conditions by Pseudomonas sp. strain ADP in aquifer sediments,” Environmental Science & Technology, vol. 32, no. 23, pp. 3789–3792, 1998.
[71]
T. Lueders and M. Friedrich, “Archaeal population dynamics during sequential reduction processes in rice field soil,” Applied and Environmental Microbiology, vol. 66, no. 7, pp. 2732–2742, 2000.
[72]
N. N. North, S. L. Dollhopf, L. Petrie, J. D. Istok, D. L. Balkwill, and J. E. Kostka, “Change in bacterial community structure during in situ biostimulation of subsurface sediment cocontaminated with uranium and nitrate,” Applied and Environmental Microbiology, vol. 70, no. 8, pp. 4911–4920, 2004.
[73]
C. Y. Wu, L. Zhuang, S. G. Zhou, F. B. Li, and X. M. Li, “Fe(III)-enhanced anaerobic transformation of 2,4-dichlorophenoxyacetic acid by an iron-reducing bacterium Comamonas koreensis CY01,” FEMS Microbiology Ecology, vol. 71, no. 1, pp. 106–113, 2010.
[74]
S. Wang and W. A. Arnold, “Abiotic reduction of dinitroaniline herbicides,” Water Research, vol. 37, no. 17, pp. 4191–4201, 2003.
[75]
J. C. Xu, J. W. Stucki, J. Wu, J. E. Kostka, and G. K. Sims, “Fate of atrazine and alachlor in redox-treated ferruginous smectite,” Environmental Toxicology and Chemistry, vol. 20, no. 12, pp. 2717–2724, 2001.
[76]
M. Alexander and B. K. Lustigman, “Biodegradation: effect of chemical structure on microbial degradation of substituted benzenes,” Journal of Agricultural and Food Chemistry, vol. 14, no. 4, pp. 410–413, 1966.
[77]
G. K. Sims and L. E. Sommers, “Degradation of pyridine derivatives in soil,” Journal of Environmental Quality, vol. 14, no. 4, pp. 580–584, 1985.
[78]
D. M. Stamper and O. H. Tuovinen, “Biodegradation of the acetanilide herbicides alachlor, metolachlor, and propachlor,” Critical Reviews in Microbiology, vol. 24, no. 1, pp. 1–22, 1998.
[79]
A. M. Cupples, R. A. Sanford, and G. K. Sims, “Dehalogenation of the herbicides bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) and ioxynil (3,5-diiodino-4-hydroxybenzonitrile) by Desulfitobacterium chlororespirans,” Applied and Environmental Microbiology, vol. 71, no. 7, pp. 3741–3746, 2005.
[80]
R. C. Doyle, D. D. Kaufman, and G. W. Burt, “Effect of dairy manure and sewage sludge on14C-pesticide degradation in soil,” Journal of Agricultural and Food Chemistry, vol. 26, no. 4, pp. 987–989, 1978.
[81]
E. Barriuso, S. Houot, and C. Serra-Wittling, “Influence of compost addition to soil on the behaviour of herbicides,” Pesticide Science, vol. 49, no. 1, pp. 65–75, 1997.
[82]
S. Houot, E. Barriuso, and V. Bergheaud, “Modifications to atrazine degradation pathways in a loamy soil after addition of organic amendments,” Soil Biology & Biochemistry, vol. 30, no. 14, pp. 2147–2157, 1998.
[83]
S. Alvey and D. E. Crowley, “Influence of organic amendments on biodegradation of atrazine as a nitrogen source,” Journal of Environmental Quality, vol. 24, no. 6, pp. 1156–1162, 1995.
[84]
J. A. Entry, K. G. Mattson, and W. H. Emmingham, “The influence of nitrogen on atrazine and 2,4-dichlorophenoxyacetic acid mineralization in grassland soils,” Biology and Fertility of Soils, vol. 16, no. 3, pp. 179–182, 1993.
[85]
F. M. Bento, F. A. O. Camargo, B. C. Okeke, and W. T. Frankenberger, “Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation,” Bioresource Technology, vol. 96, no. 9, pp. 1049–1055, 2005.
[86]
S. Liu and J. M. Suflita, “Ecology and evolution of microbial populations for bioremediation,” Trends in Biotechnology, vol. 11, no. 8, pp. 344–352, 1993.
[87]
P. J. Sturman, P. S. Stewart, A. B. Cunningham, E. J. Bouwer, and J. H. Wolfram, “Engineering scale-up of in situ bioremediation processes: a review,” Journal of Contaminant Hydrology, vol. 19, no. 3, pp. 171–203, 1995.
[88]
D. Lima, P. Viana, S. André et al., “Evaluating a bioremediation tool for atrazine contaminated soils in open soil microcosms: the effectiveness of bioaugmentation and biostimulation approaches,” Chemosphere, vol. 74, no. 2, pp. 187–192, 2009.
[89]
L. C. Strong, H. McTavish, M. J. Sadowsky, and L. P. Wackett, “Field-scale remediation of atrazine-contaminated soil using recombinant Escherichia coli expressing atrazine chlorohydrolase,” Environmental Microbiology, vol. 2, no. 1, pp. 91–98, 2000.
[90]
I. Robles-González, E. Ríos-Leal, R. Ferrera-Cerrato, F. Esparza-García, N. Rinderkenecht-Seijas, and H. M. Poggi-Varaldo, “Bioremediation of a mineral soil with high contents of clay and organic matter contaminated with herbicide 2,4-dichlorophenoxyacetic acid using slurry bioreactors: effect of electron acceptor and supplementation with an organic carbon source,” Process Biochemistry, vol. 41, no. 9, pp. 1951–1960, 2006.
[91]
G. R. Tortella, O. Rubilar, M. Cea, C. Wulff, O. Martinez, and M. C. Diez, “Biostimulation of agricultural biobeds with NPK fertilizer on chlorpyrifos degradation to avoid soil and water contamination,” Journal of Soil Science and Plant Nutrition, vol. 10, no. 4, pp. 464–475, 2010.
[92]
I. McGhee and R. G. Burns, “Biodegradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methyl-4-chlorophenoxyacetic acid (MCPA) in contaminated soil,” Applied Soil Ecology, vol. 2, no. 3, pp. 143–154, 1995.
[93]
A. D. K. McBain, E. Senior, A. Paterson, C. A. du Plessis, and I. A. Watson-Craik, “Bioremediation of 4-chloro-2-methylphenoxyacetic acid (MCPA)-contaminated soil: microcosm and pilot field studies,” South African Journal of Science, vol. 93, no. 5, pp. 226–230, 1997.
[94]
A. Liaghat and S. O. Prasher, “A lysimeter study of grass cover and water table depth effects on pesticide residues in drainage water,” Transactions of the American Society of Agricultural Engineers, vol. 39, no. 5, pp. 1731–1738, 1996.
[95]
K. Isermann, “Share of agriculture in nitrogen and phosphorus emissions into the surface waters of Western Europe against the background of their eutrophication,” Fertilizer Research, vol. 26, no. 1-3, pp. 253–269, 1990.
[96]
M. Tyagi, M. M. R. da Fonseca, and C. C. C. R. de Carvalho, “Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes,” Biodegradation, vol. 22, no. 2, pp. 221–231, 2010.