A multimodal approach is effective in analyzing biological problems critically and thus also useful in assessing cytotoxicity under chemicals assaults. In this study effects of isooctane, an organic solvent and component of gasoline produced in petroleum industries, have been explored on normal (HaCaT) and cancerous (HeLa) epithelial cells. Besides morphological alterations, impacts on viability, prime molecular expressions, and bioelectrical properties on exposure to different doses of isooctane were noted. Scanning electron microscopy and viability assay demonstrated remarkable structural alterations and cell death, respectively, in HaCaT but not in HeLa. Transcriptomic and immunocytochemical studies on E-cadherin expression also elucidated pronounced toxic effects on HaCaT. Remarkable changes on the bioelectrical properties (e.g., impedance and phase angle) of the HaCaT, in contrast to HeLa, at different temporal points on isooctane exposure also indicated cytotoxic effects in the former. Hence this study illustrated cytotoxicity of isooctane on HaCaT multidimensionally which was evaded by HeLa. 1. Introduction Profuse use of chemicals in modern times is posing serious threats to the health and environment. The number of synthetic substances to which humans are exposed has increased enormously over the last decades as well [1]. In the context of hazards, toxic effect of organic solvents (OS) is a major health concern [2] especially due to their cytotoxicity [3] and protein denaturing effects [3, 4]. Isooctane (IO) is one of such synthetic OS and needs better attention particularly to elucidate its impact on mammalian objects [5]. It is produced on a massive scale in the petroleum industry by distillation of petroleum [6] and is an important component of gasoline. IO is useful for production of ethanol with extremely low volatility [7]. Structurally it has high octane number and does not contain any aromatics, metals, or sulphur. In 2008 the worldwide market of IO was expected to be 1.0 million tons approximately [8]. The toxicity of any OS depends on the cell type and its physiological state [9]. The primary site of cytotoxic action is cytoplasmic membrane of cells [3]. Cytotoxicity of IO on microorganism like yeast has already been reported [10] but its impact on the mammalian cells is not well documented. Only the positive effect of IO in combination with acetic acid in psoriasis is reported [11]. The epithelium is the interface between body and external environment. Its tightly packed cells (namely, keratinocytes) are effective barriers between
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