Ethanol extract of Venenum Bufonis has shown many valuable bioactivities, but little is known about its effect on biofilm formation of Staphylococcus aureus. In this study, biofilm formation of S. aureus NCTC8325 with or without ethanol extract of Venenum Bufonis was tested using microtitre plate assay and Confocal Laser Scanning Microscope (CLSM) system. Results show that the biofilm formation of S. aureus with ethanol extract of Venenum Bufonis was significantly lower than that of the control group without ethanol extract of Venenum Bufonis. Meanwhile, the reduction degree was correlated to the concentration of ethanol extract of Venenum Bufonis positively. With CLSM system we can observe that looser and less biomass of the biofilm structure of the experimental group appeared than that of the control group. These results suggested that ethanol extract of Venenum Bufonis has inhibitory effect on biofilm formation of S. aureus.
References
[1]
Lowy, F.D. (1998) Staphylococcus aureus Infections. New England Journal of Medicine, 339, 520-532. http://dx.doi.org/10.1056/NEJM199808203390806
[2]
Otto, A., van Dijl, J.M., Hecker, M. and Becher, D. (2014) The Staphylococcus aureus Proteome. International Journal of Medical Microbiology, 304, 110-120. http://dx.doi.org/10.1016/j.ijmm.2013.11.007
[3]
Costerton, J.W., Montanaro, L. and Arciola, C.R. (2005) Biofilm in Implant Infections: Its Production and Regulation. The International Journal of Artificial Organs, 28, 1062-1068.
[4]
Del Pozo, J.L. and Patel, R. (2007) The Challenge of Treating Biofilm-Associated Bacterial Infections. Clinical Pharmacology and Therapeutics, 82, 204-209. http://dx.doi.org/10.1038/sj.clpt.6100247
[5]
Archer, N.K., Mazaitis, M.J., Costerton, J.W., Leid, J.G., Powers, M.E. and Shirtliff, M.E. (2011) Staphylococcus aureus Biofilms: Properties, Regulation, and Roles in Human Disease. Virulence, 2, 445-459. http://dx.doi.org/10.4161/viru.2.5.17724
[6]
Wang, S.H., Wang, Y.C., Nie, Y.L., Hai, Y.N., Sun, H.F., Yuan, Z.L. and Nan, K.J. (2013) Antiproliferative Activity of the Chinese Medicinal Compound, Delisheng, Compared with Rg3 and Gemcitabine in HepG2 Cells. Indian Journal of Pharmaceutical Sciences, 75, 578-584.
[7]
Chen, G., Jiang, X., Li, J., Duan, G., Yang, L., Zhang, Y. and Wang, F. (2014). Study on Anti-Osteosarcoma Activity of Ethanol Extract of Venenum Bufonis in Vitro. African Journal of Traditional, Complementary, and Alternative Medicines. African Networks on Ethnomedicines, 11, 73-77.
[8]
Zhang, D.M., Liu, J.S., Tang, M.K., Yiu, A., Cao, H.H., Jiang, L., Chan, J.Y., Tian, H.Y., Fung, K.P. and Ye, W.C. (2012) Bufotalin from Venenum Bufonis Inhibits Growth of Multidrug Resistant HepG2 Cells through G2/M Cell Cycle Arrest and Apoptosis. European Journal of Pharmacology, 692, 19-28. http://dx.doi.org/10.1016/j.ejphar.2012.06.045
[9]
Li, M., Yu, X., Guo, H., Sun, L., Wang, A., Liu, Q., Wang, X. and Li, J. (2014) Bufalin Exerts Antitumor Effects by Inducing Cell Cycle Arrest and Triggering Apoptosis in Pancreatic Cancer Cells. Tumour Biology, 35, 2461-2471. http://dx.doi.org/10.1007/s13277-013-1326-6
[10]
Beenken, K.E., Blevins, J.S. and Smeltzer, M.S. (2003) Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation. Infection and Immunity, 71, 4206-4211. http://dx.doi.org/10.1128/IAI.71.7.4206-4211.2003
