A dual real-time quantitative polymerase
chain reaction assay (drtqPCR) was established to detect and differentiate
between Porcine circovirus-2a (PCV-2a) and Porcine circovirus-2b (PCV-2b).
Genotype-specific primer sets and probes were designed by using sequence data
published for different PCV-2 strains. Specificity and sensitivity of the
drtqPCR were examined by using PCV-2 isolates with known genotype. Among 367
tissue samples, 44.69% (164/367) were PCV-2 positive. From 164 PCV-2 positive
samples, 10.98% (18/164), 92.56% (137/164), and 3.31% (9/164) were positive for
PCV-2a, PCV-2b, and both genotypes, respectively. These results suggest that
the dif-ferential drtqPCR can be used to detect PCV-2 and to differentiate the
2 genotypes from field sam-ples. The PCV-2 infection is quite common in swine
of Shanghai area. Furthermore, the PCV-2b infective ratio is far higher than
PCV-2a, and PCV-2a/2b mixed infections are also observed but at a lower
prevalence in Shanghai area.
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