Drought is one of the major factors limiting the yield and quality of
crops in the world. The activity of antioxidative
system to tolerate the drought stress is significant
in plants. In the present study, the activities and
isoform profiles of catalase (CАТ), ascorbate peroxidase (APX), glutathione reductase (GR), and superoxide
dismutase (SOD) were analyzed in four barley genotypes grown under soil water restriction.
Drought stress caused increase in the activities of CАТ and SOD in all studied genotypes, while APX activity
decreased. The total GR activity increased substantially in genotypes K 2778 and St.Garabag 7
and decreased in No. 77 local and St.Pallidum 596 genotypes under conditions of severe water stress. No detectable differences were
observed in the isoenzyme pattern (the appearance of a new isoenzymes and
disappearance of another one) between control plants and those subjected to
soil drought. However, intensification of corresponding isoforms in
electrophoretic spectra was observed in stressed barley leaves relative to
watered ones. The obtained results
possibly suggest that antioxidant protection in barley plants under drought
conditions could be attributed mainly to SOD and CAT.
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