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Botanical Research 2014
烟草BY-2原生质体定向伸长对离心力响应的相关性研究
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Abstract:
本研究以烟草BY-2细胞为材料，在单细胞水平探讨应力条件对植物细胞定向伸长的影响。将烟草原生质体包埋于琼脂糖凝胶块中，通过施加不同大小的离心力和在恒定的施力条件下处理不同时间分析应力与细胞定向伸长的相关性。结果表明，在恒定施力条件下，原生质体的定向伸长与施力时间密切相关，倾向以垂直于应力方向伸长，且细胞的定向伸长与应力强度存在剂量关系。细胞微管骨架与离心力的方向平行。
In this study, the protoplasts from tobacco BY-2 (Nicotiana tabacum) were used to determine whether cells can be induced to expand in a preferential direction in response to an externally applied centrifugal force. Spherical protoplasts were embedded into agarose block. Constant uniaxial pressure released by a centrifugal force was imposed on the block. The results showed that protoplasts elongated with a preferential axis oriented perpendicularly to the applied force direction, according to a certain dose-dependent relation between the intensity of centrifugal force and the stress induced responses. The microtubules with centrifuged protoplasts were found to be oriented parallel to the centrifugal force direction.
| [1] | Starr, C. and Taggart, R. (1995) Biology, the unity and diversity of life. 7th Edition, Wadsworth Publishing Company, 140-153. |
| [2] | Lloyd, C. and Buschmann, H. (2007) Plant division: Remembering where to build the wall. Current Biology, 17, 10531055. |
| [3] | Cosgrove, D.J. (1993) Wall extensibility: Its nature, measurement and relationship to plant cell growth. New Phytologist, 124, 1-23. |
| [4] | Moller, S.G. and Chuan, N.H. (1999) Interactions and intersections of plant signaling pathways. Journal of Molecular Biology, 293, 219-234. |
| [5] | Nagpal, P. and Quatrano, R.S. (1992) Isolation and characterization of a cDNA clone from Arabidopsis thaliana with partial sequence similarity to integrins. Gene, 230, 33-40. |
| [6] | Knight, M.R., Smith, S.M. and Trewavas, A.J. (1992) Wind-induced plant motion immediately increases cytosolic calcium. Proceedings of the National Academy of Sciences of the United States of America, 89, 4967-4971. |
| [7] | Haley, A., Russell, A.J., Wood, N., et al. (1995) Effect of mechanical signaling on plant cell cytosolic calcium. Proceedings of the National Academy of Sciences of the United States of America, 92, 4124-4128. |
| [8] | Trewavas, A. and Knight, M. (1994) Mechanical signaling, calcium and plant form. Plant Molecular Biology, 26, 1329-1341. |
| [9] | Braam, J. and Davis, R.W. (1990) Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related Genes in Arabidopsis. Cell, 60, 357-364. |
| [10] | Cormacl, E.M. and Braam, J. (2003) Calmodulins and related potential calciums ensors of Arabidopsis. New Phytologist, 159, 585-598. |
| [11] | Wymer, C.L., Wymer, S.A., Cosgrove, D.J. and Cyr, R.J. (1996) Plant cell growth responds to externa1 forces and the response requires intact microtubules. Plant Physiology, 110, 425-430. |
| [12] | 刘贻尧, 王伯初, 赵虎成, 等 (2000) 植物对环境应力刺激的生物学效应. 生物技术通讯, 3, 219-222. |
| [13] | Lynch, T.M. and Lintilhac, P.M. (1997) Mechanical signals in plant development: A new method for single cell studies. Developmental Biology, 181, 246-256. |
| [14] | Martin, C., Bhatt, K. and Baumann, K. (2001) Shaping in plant cells. Current Opinion in Plant Biology, 4, 540-549. |
| [15] | Reuzeau, C. and Pont-Lezica, R.F. (1995) Comparing plant and animal extracellular matrix-cytoskeleton connections: Are they alike. Protoplasma, 186, 113-121. |
| [16] | Hush, J. and Overall, R. (1991) Electrical and mechanical fields orient cortical microtubules in higher plant tissues. Cell Biology International Reports, 15, 551-560. |
| [17] | Cleary, A.L. (1995) F-actin redistributions at the division site in living Tradescantia stomatal complexes as revealed by microinjection of rhodamine-phalloidin. Protoplasma, 185, 152-165. |
| [18] | Zandomeni, K. and Schopfer, P. (1994) Mechanosensory microtubule reorientation in the epidermis of maize coleoptiles subjected to bending stress. Protoplasma, 182, 96-101. |
