The present study was undertaken to assess the motility and velocity parameters of sperm of freezable and nonfreezable ejaculates by computer-assisted sperm analyser (CASA) such as Hamilton-Thorne Semen Analyser IVOS 11 in mithun semen. Fifty ejaculates (twenty-five ejaculates each for freezable and nonfreezable semen ejaculates) were collected from ten matured mithun bulls. CASA parameters, motility parameters such as forward progressive motility (FPM) (%), nonprogressive motility (NPM) (%), total motility (TM) (%), and static sperms (SM) (%); velocity parameters such as curvilinear velocity (VCL) (μm/sec), straight line velocity (VSL) (μm/sec), average path velocity (VAP) (μm/sec), linearity (LIN) (%), straightness (STR) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (μm), and beat/cross-frequency (BCF) (Hz) were measured by CASA analyser. The result revealed that these parameters varied significantly ( ) between the freezable and nonfreezable ejaculates and freezable ejaculates have significantly ( ) higher value than nonfreezable ejaculates. It was concluded that most of the CASA parameters were significantly lower in nonfreezable ejaculates than in freezable ejaculates in mithun and confirmed that the CASA was effective for a quick and objective analysis of motility and velocity parameters in mithun semen. 1. Introduction Mithun (Bos frontalis) is a semiwild, free-range, rare bovine species present in the North-Eastern Hill (NEH) region of India and is believed to have originated more than 8000 years ago from wild Indian gaur (Bos gaurus) [1]. The animal has an important place in the social, cultural, religious, and economic life of the tribal population of NEH region. As per the livestock census of India (2007), the mithun population is decreasing gradually due to lack of suitable breeding bulls, increase in intensive inbreeding practices, and lack of suitable breeding management. Greater efforts are required from all quarters to preserve the mithun population to enhance the socioeconomic status of this region. Since mithuns are semiwild animal and not fully domesticated, natural breeding is practiced in this species with accompanied limitations like cost and disease transmission. Thus, use of artificial insemination for improvement of its pedigree is utmost essential. Etiology for infertility in male animals is varied and complex. Infertility is diagnosed by history, clinical signs, physical examination of external and internal genitalia, assessment of libido and sexual behaviour, semen collection and evaluation, and various
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