We combined the new SensititreTM MYCOTB test with the MODS assay for detection of MDR- and XDR-TB. Categorical agreement of the MODS assay with the critical concentrations at 3 days of incubation was highest for INH (91.4%) and RIF (100%) and at 5 days 86.7% and 94.6% for the fluoroquinolones and aminoglycosides, respectively. By combining these two methods, it is possible to identify MDR-TB in as little as 3 days and XDR- or pre-XDR-TB within 5 days.
References
[1]
World Health Organization (2012) Global Tuberculosis Report. World Health Organization, Geneva.
[2]
Abuali, M.M., Katariwala, R. and LaBombardi, V.J. (2012) A Comparison of the Sensititre MYCOTB Panel and the Agar Proportion Method for the Susceptibility Testing of Mycobacterium tuberculosis. European Journal of Clinical Microbiology & Infectious Diseases, 31, 835-839. http://dx.doi.org/10.1007/s10096-011-1382-z
[3]
Hall, L., Jude, K., Clark, S., Dionne, K., Merson, R., Boyer, A., Parrish, N. and Wengenack, N. (2012) Evaluation of the Sensititre® MYCOTB MIC Plate for the Susceptibility Testing of Mycobacterium tuberculosis Complex against First and Second Line Agents. Journal of Clinical Microbiology, 50, 3732-3734.
http://dx.doi.org/10.1128/JCM.02048-12
[4]
Caviedes, L., Lee, T.S., Gilman, R.H., Sheen, P., Spellman, E., Lee, E.H., Berg, D.E. and Montenegro-James, S. (2000) Rapid, Efficient Detection and Drug Susceptibility Testing of Mycobacterium tuberculosis in Sputum by Microscopic Observation of Broth Cultures. Journal of Clinical Microbiology, 38, 1203-1208.
[5]
Moore, D.A., Evans, C.A., Gilman, R.H., Caviedes, L., Coronel, J., Vivar, A., Sanchez, E., Pinedo, Y., Saravia, J.C., Salazar, C., Oberhelman, R., Hollm-Delgado, M.G., LaChira, D., Escombe, A.R. and Friedland, J.S. (2006) Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB. The New England Journal of Medicine, 355, 1539-1550. http://dx.doi.org/10.1056/NEJMoa055524
Rodrigues, C., Jani, J., Shenai, S., Thakkar, P., Siddiqi, S. and Mehta, A. (2008) Drug Susceptibility Testing of Mycobacterium tuberculosis against Second-Line Drug Using the Bactec MGIT 960 System. International Journal of Tuberculosis and Lung Disease, 12, 1449-1455.
[8]
Clinical and Laboratory Standards Institute (2011) Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard. 2nd Edition, CLSI Document M24-A2, Clinical Laboratory Standards Institute, Wayne.
