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Expression of SOX3 and SOX9 Genes in Gonads of Blue Gourami

DOI: 10.4236/abc.2014.45037, PP. 322-330

Keywords: SOX3, SOX9, mRNA and Blue Gourami

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Abstract:

In vertebrates, SOX (SRY-related HMG box) genes are thought to be due to major gene duplication events, initially occurring during early stages of metazoan evolution and later during the transition between non-vertebrate chordates and vertebrates. The aim of this study is to examine SOX3 and SOX9 transcription in oogensis and spermatogenesis in a fish model, the blue gourami (Trichogaster trichopterus). In females during oogenesis, SOX9 mRNA levels were lower compared to SOX3 mRNA levels. In males, SOX9 mRNA levels were higher in testes compared to SOX3 mRNA levels, however no significant differences between SOX3 and SOX9 mRNA levels were observed in the gonads of males that were kept under non-reproductive conditions compared to males kept with females under reproductive conditions and that were nest-builders.

References

[1]  Hong, C.S. and Saint-Jeannet, J.P. (2005) Sox Proteins and Neural Crest Development. Seminars in Cell & Developmental Biology, 16, 694-703.
http://dx.doi.org/10.1016/j.semcdb.2005.06.005
[2]  Betancur, P., Bronner-Fraser, M. and Sauka-Spengler, T. (2010) Genomic Code for Sox10 Activation Reveals a Key Regulatory Enhancer for Cranial Neural Crest, Proceedings of the National Academy of Sciences of the United States, 107, 3570-3575.
http://dx.doi.org/10.1073/pnas.0906596107
[3]  Laudet, V., Stehelin, D. and Clevers, H. (1993) Ancestry and Diversity of the HMG Box Superfamily. Nucleic Acids Research, 21, 2493-2501.
http://dx.doi.org/10.1093/nar/21.10.2493
[4]  Prior, H.M. and Walter, M.A. (1996) SOX Genes: Architects of Development. Molecular Medicine, 2, 405-412.
[5]  Dehal, P. and Boore, J.L. (2005) Two Rounds of Whole Genome Duplication in the Ancestral Vertebrate, PLoS Biology, 3, e314.
http://dx.doi.org/10.1371/journal.pbio.0030314
[6]  Escriva, H., Manzon, L., Youson, J. and Laudet, V. (2002) Analysis of Lamprey and Hagfish Genes Reveals a Complex History of Gene Duplications during Early Vertebrate Evolution. Molecular Biology and Evolution, 19, 1440-1450.
http://dx.doi.org/10.1093/oxfordjournals.molbev.a004207
[7]  Wright, E.M., Snopek, B. and Koopman, P. (1993) Seven New Members of the Sox Gene Family Expressed during Mouse Development. Nucleic Acids Research, 21, 744.
http://dx.doi.org/10.1093/nar/21.3.744
[8]  Bowles, J., Schepers, G. and Koopman, P. (2000) Phylogeny of the SOX Family of Developmental Transcription Factors Based on Sequence and Structural Indicators. Developmental Biology, 227, 239-255.
http://dx.doi.org/10.1006/dbio.2000.9883
[9]  Uy, B.R., Simoes-Costa, M., Sauka-Spengler, T. and Bronner, M.E. (2012) Expression of Sox Family Genes in Early Lamprey Development. The International Journal of Developmental Biology, 56, 377-383.
http://dx.doi.org/10.1387/ijdb.113416bu
[10]  Collignon, J., Sockanathan, S., Hacker, A., Cohen-Tannoudji, M., Norris, D., Rastan, S., Stevanovic, M., Goodfellow, P.N. and Lovell-Badge, R. (1996) A Comparison of the properties of Sox-3 with Sry and Two Related Genes, Sox-1 and Sox-2. Development, 122, 509-520.
[11]  Graves, J.A. (2006) Sex Chromosome Specialization and Degeneration in Mammals. Cell, 124, 901-914.
http://dx.doi.org/10.1016/j.cell.2006.02.024
[12]  Weiss, J., Meeks, J.J., Hurley, L., Raverot, G., Frassetto, A. and Jameson, J.L. (2003) Sox3 Is Required for Gonadal Function, but Not Sex Determination, in Males and Females. Molecular and Cellular Biology, 23, 8084-8091.
http://dx.doi.org/10.1128/MCB.23.22.8084-8091.2003
[13]  Sekido, R. and Lovell-Badge, R. (2008) Sex Determination Involves Synergistic Action of SRY and SF1 on a Specific Sox9 Enhancer. Nature, 453, 930-934.
http://dx.doi.org/10.1038/nature06944
[14]  Uno, Y., Nishida, C., Oshima, Y., Yokoyama, S., Miura, I., Matsuda, Y. and Nakamura, M. (2008) Comparative Chromosome Mapping of Sex-Linked Genes and Identification of Sex Chromosomal Rearrangements in the Japanese Wrinkled Frog (Rana rugosa, Ranidae) with ZW and XY Sex Chromosome Systems. Chromosome Research, 16, 637-647.
http://dx.doi.org/10.1007/s10577-008-1217-7
[15]  Takehana, Y., Demiyah, D., Naruse, K., Hamaguchi, S. and Sakaizumi, M. (2007) Evolution of Different Y Chromosomes in Two Medaka Species, Oryzias dancena and O. latipes. Genetics, 175, 1335-1340.
http://dx.doi.org/10.1534/genetics.106.068247
[16]  Okuda, Y., Yoda, H., Uchikawa, M., Furutani-Seiki, M., Takeda, H., Kondoh, H. and Kamachi, Y. (2006) Comparative Genomic and Expression Analysis of Group B1 Sox Genes in Zebrafish Indicates Their Diversification during Vertebrate Evolution. Developmental Dynamics, 235, 811-825.
http://dx.doi.org/10.1002/dvdy.20678
[17]  Jackson, K., Goldberg, D., Ofir, M., Abraham, M. and Degani, G. (1999) Blue Gourami (Trichogaster trichopterus) Gonadotropic Beta Subunits (I and II) cDNA Sequences and Expression during Oogenesis. Journal of Molecular Endocrinology, 23, 177-187.
http://dx.doi.org/10.1677/jme.0.0230177
[18]  Levy, G., Gothilf, Y. and Degani, G. (2009) Brain Gonadotropin Releasing Hormone3 Expression Variation during Oogenesis and Sexual Behavior and Its Effect on Pituitary Hormonal Expression in the Blue Gourami. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 154, 241-248.
http://dx.doi.org/10.1016/j.cbpa.2009.06.010
[19]  Levy, G., Jackson, K. and Degani, G. (2010) Association between Pituitary Adenylate Cyclase-Activating Polypeptide and Reproduction in the Blue Gourami. General and Comparative Endocrinology, 166, 83-93.
http://dx.doi.org/10.1016/j.ygcen.2009.09.015
[20]  Degani, G. (2001) Blue Gourami (Trichogaster trichopterus) Model for Labyrinth Fish. Laser Pages Publishing, Jerusalem.
[21]  Jackson, K., Goldberg, D., Ofir, M., Abraham, M. and Degani, G. (1999) Blue Gourami (Trichogaster trichopterus) Gonadotropic Beta Subunits (I and II) cDNA Sequences and Expression during Oogenesis. Journal of Molecular Endocrinology, 23, 177-187.
http://dx.doi.org/10.1677/jme.0.0230177
[22]  Pfaffl, M.W. (2001) A New Mathematical Model for Relative Quantification in Real-Time RT-PCR. Nucleic Acids Research, 29, e45.
http://dx.doi.org/10.1093/nar/29.9.e45
[23]  Muller, P.Y., Janovjak, H., Miserez, A.R. and Dobbie, Z. (2002) Processing of Gene Expression Data Generated by Quantitative Real-Time RT-PCR. Biotechniques, 32, 1372-1379.
[24]  Chiang, E.F., Pai, C.I., Wyatt, M., Yan, Y.L., Postlethwait, J. and Chung, B. (2001) Two Sox9 Genes on Duplicated Zebrafish Chromosomes: Expression of Similar Transcription Activators in Distinct Sites. Developmental Biology, 231, 149-163.
http://dx.doi.org/10.1006/dbio.2000.0129
[25]  Zhou, R., Liu, L., Guo, Y., Yu, H., Cheng, H., Huang, X., Tiersch, T.R. and Berta, P. (2003) Similar Gene Structure of Two Sox9a Genes and Their Expression Patterns during Gonadal Differentiation in a Teleost Fish, Rice Field Eel (Monopterus albus). Molecular Reproduction and Development, 66, 211-217.
http://dx.doi.org/10.1002/mrd.10271
[26]  Kluver, N., Kondo, M., Herpin, A., Mitani, H. and Schartl, M. (2005) Divergent Expression Patterns of Sox9 Duplicates in Teleosts Indicate a Lineage Specific Subfunctionalization. Development Genes and Evolution, 215, 297-305.
http://dx.doi.org/10.1007/s00427-005-0477-x
[27]  Du, Q.Y., Wang, F.Y., Hua, H.Y. and Chang, Z.J. (2007) Cloning and Study of Adult-Tissue-Specific Expression of Sox9 in Cyprinus carpio. Journal of Genetics, 86, 85-91.
http://dx.doi.org/10.1007/s12041-007-0013-z
[28]  Chiang, E.F., Yan, Y.L., Guiguen, Y., Postlethwait, J. and Chung, B. (2001) Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish Chromosomes Are Expressed in Ovary or Brain. Molecular Biology and Evolution, 18, 542-550.
http://dx.doi.org/10.1093/oxfordjournals.molbev.a003833
[29]  Bagheri-Fam, S., Sinclair, A.H., Koopman, P. and Harley, V.R. (2010) Conserved Regulatory Modules in the Sox9 Testis-Specific Enhancer Predict Roles for SOX, TCF/LEF, Forkhead, DMRT, and GATA Proteins in Vertebrate Sex Determination. The International Journal of Biochemistry & Cell Biology, 42, 472-477.
http://dx.doi.org/10.1016/j.biocel.2009.07.001
[30]  Chalmel, F., Lardenois, A., Georg, I., Barrionuevo, F., Demougin, P., Jegou, B., Scherer, G. and Primig, M. (2013) Genome-Wide Identification of Sox8-, and Sox9-Dependent Genes during Early Post-Natal Testis Development in the Mouse. Andrology, 1, 281-292.
http://dx.doi.org/10.1111/j.2047-2927.2012.00049.x
[31]  Zhou, R., Cheng, H., Zhang, Q., Guo, Y., Cooper, R.K. and Tiersch, T.R. (2002) SRY-Related Genes in the Genome of the Rice Field Eel (Monopterus albus). Genetics Selection Evolution, 34, 129-137.
http://dx.doi.org/10.1186/1297-9686-34-1-129
[32]  Degani, G., Jackson, K., Goldberg, D., Sarfati, R. and Avtalion, R.R. (2003) βFSH, βLH and Growth Hormone Gene Expression in Blue Gourami (Trichogaster trichopterus, Pallas 1770) during Spermatogenesis and Male Sexual Behavior. Zoological Science, 20, 737-743.
http://dx.doi.org/10.2108/zsj.20.737
[33]  Degani, G. (1993) Reproduction Control in Multi-Spawning Asynchronic Trichogaster trichopterus (Pallas) as a Model for the Anabantidae Family. Trends in Comparative Biochemistry & Physiology, 1, 1269-1275.
[34]  Raghuveer, K. and Senthilkumaran, B. (2010) Isolation of Sox9 Duplicates in Catfish: Localization, Differential Expression Pattern during Gonadal Development and Recrudescence, and hCG-Induced Up-Regulation of Sox9 in Testicular Slices. Reproduction, 140, 477-487.
http://dx.doi.org/10.1530/REP-10-0200
[35]  Kanda, H., Kojima, M., Miyamoto, N., Ito, M., Takamatsu, N., Yamashita, S. and Shiba, T. (1998) Rainbow Trout Sox24, a Novel Member of the Sox Family, Is a Transcriptional Regulator during Oogenesis. Gene, 211, 251-257.
http://dx.doi.org/10.1016/S0378-1119(98)00100-0

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