The Nu River Project comprised of 13-dam cascade is one of the biggest
hydropower development projects yet conceived in China. However, hydropower
development will inevitably result in the local ecological environment and
biological material impact, particularly the effects on fish. Glyptothorax zainaensis is weak in
swimming with suction cup and is extremely sensitive in the hydrological
characteristics and water quality changes, so that it can be considered as a
water quality monitor of indicator organism. Currently, with the need for
conservation, fishery restoration and so on, there is a great demand for
genetic information on Glyptothorax
zainaensis. Fourty microsatellite loci were isolated, characterized and
evaluated from Glyptothorax zainaensis.
A total of 91 different alleles were observed in the 303 wild Glyptothorax zainaensis, and the
percentage of polymorphic bands ranged from 41.8% to 66.7%, with an average of
90.1%. The genetic diversity value was significantly huge with the expected
heterozygosity ranging from 0.578 to 0.706, the observed heterozygosity value
ranging from 0.923 to 0.980 and PIC value ranging from 0.418 to 0.644, which
showed that the wild Glyptothorax
zainaensis population had a high level of genetic diversity. Analysis of
molecular variance (AMOVA) and pairwise Fst showed a significant level of
genetic differentiation (P < 0.01) among the populations accounting for
40.2% of the total variance. Additionally, results derived from different
statistical techniques with different software packages included cluster
analysis on NJ tree. Apart from such molecular approaches, we opposed four ways
to strengthen the effective resources protection.
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