The multilayer
of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly
was investigated for its antibacterial activity against Enterobactercloaceae, Staphylococcusaureus and Enterococcusfaecalis 29505 for potential use as coating
on biomaterial surface. All the multilayers exhibited growth inhibition. PEI/Iota carrageenan multilayer was effective
in inhibiting the growth of the E.cloaceae, S.aureus and E.faecalis while PEI/Lambda carrageenan was effective
in inhibiting the growth of E.cloaceae. Results of the paper strip test
for combined action of carrageenan and PEI showed synergism with regards to bacterial
growth inhibition. The multilayers had also contact-killing effect with the test
organisms. The multilayer was also characterized by atomic force microscopy (AFM),
X-ray photoelectron spectroscopy (XPS) and biomolecular interaction analysis.
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