A field experiment was
conducted in Taiwan to measure the cross-pollination (CP) rate of maize pollen
recipients from pollen sources using phenotypic marker and to determine the
isolation dis- tance between the 2 maize varieties. A waxy variety (Black Pearl)
with purple kernels simulated the genetically modified (GM) pollen donor, and
another waxy variety (White Pearl) with white kernels simulated the non-GM
recipient. For the first crop, the total area was approximately1.5 hawith a pollen source and recipient
acreage ratio of approximately 1:32. For the second crop, the total area was
approximately1.83 hawith a ratio of approximately 1:17.3. The source fields were surrounded by the
recipient fields for 2 crop seasons. The results showed that the rate of CP was
<0.05% beyond15 mupwind and84.8 mdownwind in all crop seasons. The CP rate was below 5% at a distance of10min the downwind direction. A sample with 0.24% CP was recorded at107.3 mdownwind; however, the CP rate
was 0% at68 mupwind. Three empirical
models were used, that is, exponential, log/log and log/log, and a simplified
Gaussian Plume model, to examine the relationship between the CP rates and the
source-field distances. All of the models were appropriate for predicting CP
rates, and the Gaussian Plume model performed better compared to the empirical
models. The results show that it is possible to control CP from foreign pollen
by using an appropriate isolation distance.
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