Objective:
To explore the effects of Anwei decoction (AD) on the protein expression of TFF
in rats with chronic atrophic gastritis(CAG). Methods: Forty-eight healthy rats
were randomly divided into 4 groups: normal control group, pathologic model
group, Anwei Decoction group, and Weifuchun group. CAG was induced in rats with
N-methy-N-nitro-N-nitrogua-nidine (MNNG). The protein expression of TFF in
rats’ gastric mucosa was determined by immunohistochemistry. Results: Compared
with that in the normal control group, the protein expression of TFF1 was
significantly enhanced in the pathologic model, Anwei Decoction and Weifuchun
groups (both P < 0.01). The protein expression level of TFF1 was significantly higher in the Anwei Decoction
group than in the Weifuchun group (P < 0.01). Compared with the normal
control group, the protein expression of TFF2 was significantly enhanced in the
pathologic model, Anwei Decoction and Weifuchun groups (both P < 0.01). The
protein expression level of TFF2 was significantly higher in the Anwei
Decoction group than in the Weifuchun group (P < 0.01). In comparison with
the pathologic model group, the protein
expression of TFF3 was remarkably reduced in Anwei Decoction and
Weifuchun groups (both P < 0.01). but there was no difference between the
group of Anwei decoction and the group of Weifuchun (P > 0.05). Conclusion:
Anwei decoction may be effective in the treatment of CAG by enhancing the
protein expression of TFF1, TFF2 while reducing that of TFF3 in gastric mucosas.
References
[1]
Gastroenterology Branch of Chinese Medical Association (2006) Consensus on chronic gastritis in China (Part I) (2006, Shanghai.) Chinese Journal of Digestion, 27, 45-49.
[2]
de Paulsen, N., Brychzy, A., Fournier, M.-C., et al. (2001) Role of transforming growth factor-alpha in von Hippel-Lindau (VHL)-/-clear cell renal carcinoma cell proliferation: A possible mechanism coupling VHL tumor suppressor inactivation and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America, 98, 1387-1392.
[3]
Lin, S. (2001) Clinical series of 100 traditional Chinese medical doctors in 100 years—Lin Peixiang. China Traditional Chinese Medicine Press, Beijing, 10, 99-117.
[4]
Wang, S.W., Shi, X.Y. and Huang, C.G. (2001) Pharmacodynamics research and evaluation of traditional Chinese medicine. Shaanxi Science and Technology Press, Xi’an, 315-317.
[5]
Xu, W.W. and Wang, M.Q. (2003) Observation of the curative effects of Weifuchun Tablets on chronic atrophic gastritis. Zhejiang Journal of Combination of Chinese Traditional and Western Medicine, 13, 505-506.
[6]
Poulsen, S.S., Kissow, H., Hare, K., Hartmann, B. and Thim, L. (2005) Luminal and parenteral TFF2 and TFF3 dimer andmonomer in two models of experimental colitis inthe rat. Regulatory Peptides, 126, 163-171.
http://dx.doi.org/10.1016/j.regpep.2004.09.007
[7]
Leung, W.K., Yu, J., Chan, F.K., To, K.F., Chan, M.W., Ebert, M.P., Ng, E.K., Chung, S.C., Malfertheiner, P. and Sung, J.J. (2002) Ex-pression of trefoil peptides (TFF1, TFF2, and TFF3) in gastric carcinomas, intestinal metaplasia , and non-neoplastic gastric tissues. The Journal of Pathology, 197, 582-588.
http://dx.doi.org/10.1002/path.1147
[8]
Suárez, C., Vizoso, F., Rodríguez, J.C., García, I., Raigoso, P., Allende, M.T., García-Muíz, J.L. and GarcíaMorán, M. (2001) Prognostic significance of cytosolic pS2 protein con-tent in gastric cancer. The International Journal of Biological Markers, 16, 37-44
[9]
Shiemann, U., Konturek, J., Assert, R., et al. (2002) mRNA expression of EGF receptor ligands in atrophic gastritis before and after helicobacter pylori eradication. Medical Science Monitor, 8, 53-58.
[10]
Alison, M.R., Chinery, R., Poulsom, R., et al. (1995) Experiment alulceration leads to sequential expression of spasmolytic polypeptide, intestinal trefoil factor, epidermal growth factor and transforming growth factor alpha mRNAs in rat stomach. The Journal of Pathology, 175, 405-414. http://dx.doi.org/10.1002/path.1711750408
[11]
Nie, S.N., Sun, H.C., Wu, X.H., et al. (2005) The regulation of trefoil peptides on gastric adaptive cytoprotection. Chinese Critical Care Medicine, 17, 302
[12]
Susumu, A.K., Yasukazu, O., Toshiaki, G., et al. (2011) Tests for serum levels of trefoil factor family proteins can improve gastric cancer screening. Gastroenterology, 141, 837-845, e7.
[13]
Im, S., Yoo, C.Y., Jung, J.-H., et al. (2013) Reduced expression of TFF1 and increased ex-pression of TFF3 in gastric cancer: Correlation with clinicopathological parameters and prognosis. Inter-national Journal of Medical Sciences, 10, 133-140.