The aim of this experiment was to investigate the effect of 17 -estradiol on cutaneous wound healing in 12-week ovariectomized (OVX) female mice. Eight-week-old female mice were divided into three groups: administration of 17 -estradiol after OVX (OVX + 17 -estradiol), OVX, and sham (SHAM). Four weeks after surgery, the mice received two full-thickness cutaneous wounds. 17 -Estradiol at 0.01？g/day was administered on the backs of mice in the OVX + 17 -estradiol group every day. Plasma 17 -estradiol level in the OVX + 17 -estradiol group was thus significantly higher than in the SHAM and OVX groups, but there was no significant difference between SHAM and OVX groups. The ratio of wound area was not significantly different among the three groups. However, the period required to reach a ratio of wound area of 0.15 in the OVX + 17 -estradiol group was significantly shorter than in the SHAM and OVX groups. These results indicate that cutaneous wound healing in young OVX mice was promoted by the administration of 17 -estradiol compared with that in SHAM and OVX mice without such administration, but there was no difference between the latter two groups that did not differ in 17 -estradiol level. 1. Introduction Cutaneous wound healing is a complex tightly orchestrated response to injury, carefully regulated at temporal and spatial levels [1]. With advanced age in humans and rodents, this series of events becomes disrupted and healing is delayed [2–4]. Recently, it has come to light that this series of events is also disrupted by estrogenic sex steroids in the healing of acute cutaneous wounds [5]. In young female rodents, that had undergone ovariectomy (OVX), cutaneous wound healing was delayed by systemic reduced estrogen compared with that in SHAM [6–15], whereas exogenous estrogen treatment reversed this delay by decreasing wound area [6–15], reducing local production of the inflammatory cells neutrophils or macrophages [7–11, 13], as well as the proinflammatory cytokine TNF- [7–11], and promoting reepithelialization [8, 10–13] and collagen deposition [6]. Therefore, previous research suggested that estrogen promotes cutaneous wound healing in OVX female rodents by several effects. However, previous work evaluating the effect of estrogen used only 8- to 10-week-old female mice, and, to the best of our knowledge, no research using aged female mice has been presented. Therefore, we investigated the effect of 17 -estradiol on cutaneous wound healing using 24-week OVX female mice [16]. We clarified that exogenous and continuous 17 -estradiol administration
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