Due to the widespread use of silver nanoparticles in consumer products, the toxicity of silver nanoparticles has also been studied in relation to their application. However, most genotoxicity studies of silver nanoparticles have been performed in vitro. Therefore, this study evaluated the DNA damage to lung cells caused by repeated inhalation of silver nanoparticles. Male Sprague Dawley rats were exposed to silver nanoparticles for 12 weeks in a whole-body inhalation chamber. The animals were divided into one control group and three dose groups that were exposed to silver nanoparticles (14–15 nm diameter) at concentrations of 0.66 × 10 6 particles/cm 3 (49 μg/m 3, low dose), 1.41 × 10 6 particles/cm 3 (117 μg/m 3, middle dose), and 3.24 × 10 6 particles /cm 3 (381 μg/m 3, high dose), respectively, for six hours/day over 12 weeks. The rats were sacrificed after the 12-week exposure period and the DNA damage assessed using a Comet assay of cells obtained from the right lungs. The olive tail moment values were 2.93 ± 0.19, 3.81 ± 0.23, 3.40 ± 0.22, and 5.16 ± 0.32 for the control, low-, middle-, and high-dose groups, respectively. Although no dose-dependent results were observed, a significant increase in the level of DNA damage was noted for the high-dose group.
References
[1]
Woodrow Wilson International Center for Scholars. A Nanotechnology Consumer Products Inventory. Available online: http://www.nanotechproject.org/consumerproducts (accessed on 25 January 2013).
[2]
Sung, J.H.; Ji, J.H.; Song, K.S.; Lee, J.H.; Choi, K.H.; Lee, S.H.; Yu, I.J. Acute inhalation toxicity of silver nanoparticles. Toxicol. Ind. Health 2010, 27, 149–154.
[3]
Ji, J.H.; Jung, J.H.; Kim, S.S.; Yoon, J.U.; Park, J.D.; Choi, B.S.; Chung, Y.H.; Kwon, I.H.; Jeong, J.; Han, B.S.; et al. Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague Dawley Rats. Inhal. Toxicol. 2007, 19, 857–871, doi:10.1080/08958370701432108.
[4]
Sung, J.H.; Ji, J.H.; Yun, J.U.; Kim, D.S.; Song, M.Y.; Jeong, J.; Han, B.S.; Han, J.H.; Chung, Y.H.; Kim, J.; et al. Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles. Inhal. Toxicol. 2008, 20, 567–574, doi:10.1080/08958370701874671.
[5]
Sung, J.H.; Ji, J.H.; Park, J.D.; Yoon, J.U.; Kim, D.S.; Jeon, K.S.; Song, M.Y.; Jeong, J.; Han, B.S.; Han, J.H.; et al. Subchronic inhalation toxicity of silver nanoparticles. Toxicol. Sci. 2009, 108, 452–461, doi:10.1093/toxsci/kfn246.
[6]
Song, K.S.; Sung, J.H.; Ji, J.H.; Lee, J.H.; Lee, J.S.; Ryu, H.R.; Lee, J.K.; Chung, Y.H.; Park, H.M.; Shin, B.S.; et al. Recovery from silver-nanoparticle-exposure-induced lung inflammation and lung function changes in Sprague Dawley Rats. Nanotoxicology 2013, 7, 169–180, doi:10.3109/17435390.2011.648223.
[7]
Kim, Y.S.; Kim, J.S.; Cho, H.S.; Rha, D.S.; Kim, J.M.; Park, J.D.; Choi, B.S.; Lim, R.; Chang, H.K.; Chung, Y.H.; et al. Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhal. Toxicol. 2008, 20, 575–583, doi:10.1080/08958370701874663.
[8]
Kim, J.S.; Sung, J.H.; Ji, J.H.; Song, K.S.; Lee, J.H.; Kang, C.S.; Yu, I.J. In vivo genotoxicity of silver nanoparticles after 90 day silver nanoparticle inhalation exposure. Saf. Health Work 2011, 2, 65–69, doi:10.5491/SHAW.2011.2.1.65.
[9]
Wise, J.P., Sr.; Goodale, B.C.; Wise, S.S.; Craig, G.A.; Pongan, A.F.; Walter, R.B.; Thompson, W.D.; Ng, A.K.; Aboueissa, A.M.; Mitani, H.; et al. Silver nanospheres are cytotoxic and genotoxic to fish cells. Aquat. Toxicol. 2010, 97, 34–41, doi:10.1016/j.aquatox.2009.11.016.
[10]
Asharani, P.V.; Hande, M.P.; Valiyaveettil, S. Anti-proliferative activity of silver nanoparticles. BMC Cell Biol. 2009, 10, 65, doi:10.1186/1471-2121-10-65.
[11]
Hussain, S.M.; Hess, K.L.; Gearhart, J.M.; Geiss, K.T.; Schlager, J.J. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol. In Vitro 2005, 19, 975–983, doi:10.1016/j.tiv.2005.06.034.
[12]
Kim, S.; Choi, J.E.; Choi, J.; Chung, K.H.; Park, K.; Yi, J.; Ryu, D.Y. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol. In Vitro 2009, 23, 1076–1084, doi:10.1016/j.tiv.2009.06.001.
[13]
Piao, M.J.; Kang, K.A.; Lee, I.K.; Kim, H.S.; Kim, S.; Choi, J.Y.; Choi, J.; Hyun, J.W. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol. Lett. 2011, 201, 92–100, doi:10.1016/j.toxlet.2010.12.010.
[14]
Yoon, K.Y.; Hoon, B.J.; Park, J.H.; Hwang, J. Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Sci. Total Environ. 2007, 373, 572–575, doi:10.1016/j.scitotenv.2006.11.007.
[15]
Lu, W.; Senapati, D.; Wang, S.; Tovmachenko, O.; Singh, A.K.; Yu, H.; Ray, P.C. Effect of surface coating on the toxicity of silver nanomaterials on human skin keratinocytes. Chem. Phys. Lett. 2010, 487, 92–96, doi:10.1016/j.cplett.2010.01.027.
[16]
Kim, J.S.; Song, K.S.; Sung, J.H.; Ryu, H.R.; Choi, B.G.; Cho, H.S.; Lee, J.K.; Yu, I.J. Genotoxicity, acute oral and dermal toxicity, eye and dermal irritation and corrosion and skin sensitisation evaluation of silver nanoparticles. Nanotoxicology 2013, 7, 953–960, doi:10.3109/17435390.2012.676099.
[17]
Arora, S.; Jain, J.; Rajwade, J.M.; Paknikar, K.M. Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells. Toxicol. Appl. Pharmacol. 2009, 236, 310–318, doi:10.1016/j.taap.2009.02.020.
[18]
Xia, T.; Kovochich, M.; Brant, J.; Hotze, M.; Sempf, J.; Oberley, T.; Sioutas, C.; Yeh, J.I.; Wiesner, M.R.; Nel, A.E. Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett. 2006, 6, 1794–1807, doi:10.1021/nl061025k.
[19]
Chi, Z.; Liu, R.; Zhao, L.; Qin, P.; Pan, X.; Sun, F.; Hao, X. A new strategy to probe the genotoxicity of silver nanoparticles combined with cetylpyridine bromide. Spectrochim. Acta A 2009, 72, 577–581, doi:10.1016/j.saa.2008.10.044.
[20]
Jung, J.H.; Oh, H.C.; Noh, H.S.; Ji, J.H.; Kim, S.S. Metal nanoparticle generation using a small ceramic heater with a local heating area. J. Aerosol Sci. 2006, 37, 1662–1670, doi:10.1016/j.jaerosci.2006.09.002.
[21]
National Institute for Occupational Safety and Health (NIOSH). NIOSH Manual of Analytical Methods, Method No 7300; Centers for Disease Control and Prevention: Cincinnati, OH, USA, 2003.
[22]
Kumaravel, T.S.; Jha, A.N. Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutat. Res. 2006, 605, 7–16, doi:10.1016/j.mrgentox.2006.03.002.
