We have tried to microencapsulate β-carotene with curdlan of a thermogelation type polysaccharide. Microcapsules were prepared by utilizing reverse dispersion, in which salada oil was the continuous phase (O’) and the curdlan water slurry (W) was the dispersed phase. β-carotene (O) as a core material was broken into fine oil droplets in the dispersed phase to form the (O/W) dispersion. The (O/W) dispersion was poured in the continuous phase (O’) and stirred to form the (O/W)/O’ dispersion at room temperature and then, temperature of the dispersion was raised to 80 °C to prepare curdlan-microcapusles containing β-carotene. In this microencapsulation process, the concentrations of curdlan and oil soluble surfactant and the impeller speed to form the (O/W)/O’ dispersion were mainly changed stepwise. We were able to prepare microcapsules by the microencapsulation method adopted here. The content of core material was increased with the curdlan concentration and decreased with the impeller speed and the oil soluble surfactant concentration. With the curdlan concentration, the drying rate of microcapsules was decreased and the retention ability for water was increased due to the stable preservation of β-carotene.
References
[1]
Fuchigami, K.; Taguchi, Y.; Tanaka, M. Preparation of microcapsules containing TMBA (1,3,5-trimethylbarbituric acid) by the drying-in-liquid method and its application. J. Appl. Polym. Sci. 2008, 110, 2145–2152, doi:10.1002/app.28825.
[2]
Hosogai, K.; Tanaka, M. Effect of impeller diameter on mean droplet diameter in circular loop reactor. Can. J. Chem. Eng. 1992, 70, 645–653, doi:10.1002/cjce.5450700405.
[3]
Taguchi, Y.; Tanaka, M. Microencapsulation of hydrophilic solid powder as fire retardant agent with epoxy resin by droplet coalescence method. J. Appl. Polym. Sci. 2008, 110, 1671–1676, doi:10.1002/app.28211.
[4]
King, W.J.; Pytel, N.J.; Ng, K.; Murphy, W.L. Triggered drug release from dynamic microspheres via a protein conformational change. Macromol. Biosci. 2010, 10, 580–584, doi:10.1002/mabi.200900382.
[5]
Piacentini, E.; Giorno, L.; Dragosavac, M.M.; Vladisavljevic, G.T.; Holdich, R.G. Microencapsulation of oil droplets using cold water fish gelatine/gum arabic complex coacervation by membrane emulsification. Food Res.Int. 2013, 53, 362–372, doi:10.1016/j.foodres.2013.04.012.
[6]
Chang, C.P.; Leung, T.K.; Lin, S.M.; Hsu, C.C. Release properties on gelatin-gum arabic microcapsules containing camphor oil with added polystyrene. Colloids Surf. B 2006, 50, 136–140, doi:10.1016/j.colsurfb.2006.04.008.
[7]
Takahashi, M.; Taguchi, Y.; Tanaka, M. Microencapsulation of hydrophilic solid powder as a fire retardant by the method of in situ gelation in droplets using a non-aqueous solvent as the continuous phase. Polym. Polym. Compos. 2009, 17, 83–90.
[8]
Takahashi, T.; Taguchi, Y.; Tanaka, M. Preparation of polyurea microcapsules containing pyrethroid insecticide with HMDI isocyanurate. J. Appl. Polym. Sci. 2008, 107, 2000–2006, doi:10.1002/app.27238.
[9]
Tanaka, M. Key Points in Preparation of Nano/Microcapsules; Technosystems: Tokyo, Japan, 2008.
[10]
Michael, B.; Ulrich, K. Microencapsulation of bioactive bilberry anthocyanins by means of whey protein gels. Procedia Food Sci. 2011, 1, 2047–2056, doi:10.1016/j.profoo.2011.10.006.
[11]
Betz, M.; Steiner, B.; Schantz, M.; Oidtmann, J.; Mader, K.; Richling, E.; Kulozik, U. Antioxidant capacity of bilberry extract microencapsulated in whey protein hydrogels. Food Res. Int. 2012, 47, 51–57, doi:10.1016/j.foodres.2012.01.010.
[12]
Tanaka, M.; Nakata, K.; Oyama, Y. Coalescence of dispersed liquid droplets in a batch type of stirred tank reactor. Kagaku Kogaku Ronbunshu 1970, 34, 893–897, doi:10.1252/kakoronbunshu1953.34.893.
[13]
Yokoyama, H.; Liu, M.; Taguchi, Y.; Tanaka, M. Effect of viscosity of shell solution on the content of solid powder as core material in microencapsulation by the drying-in-liquid method. J. Appl. Polym. Sci. 2008, 109, 1585–1593, doi:10.1002/app.24765.
[14]
Yokoyama, H.; Taguchi, Y.; Tanaka, M. Preparation of microcapsules by drying in liquid using EPS and the effect of preparation conditions on the inner structure. Kagaku Kogaku Ronbunshu 2007, 33, 363–368, doi:10.1252/kakoronbunshu.33.363.
