Experimental Study of Membrane Fouling during Crossflow Microfiltration of Yeast and Bacteria Suspensions: Towards an Analysis at the Microscopic Level
Microfiltration of model cell suspensions combining macroscopic and microscopic approaches was studied in order to better understand microbial membrane fouling mechanisms. The respective impact of Saccharomyces cerevisiae yeast and Escherichia coli bacteria on crossflow microfiltration performances was investigated using a multichannel ceramic 0.2 μm membrane. Pure yeast suspensions (5 μm ovoid cells) and mixtures of yeast and bacteria (1 to 2.5 μm rod shape cells) were considered in order to analyse the effect of interaction between these two microorganisms on fouling reversibility. The resistances varied significantly with the concentration and characteristics of the microorganisms. Membrane fouling with pure yeast suspension was mainly reversible. For yeast and bacteria mixed suspensions (6 g L ？1 yeast concentration) the increase in bacteria from 0.15 to 0.30 g L ？1 increased the percentage of normalized reversible resistance. At 10 g L ？1 yeast concentration, the addition of bacteria tends to increase the percentage of normalized irreversible resistance. For the objective of performing local analysis of fouling, an original filtration chamber allowing direct in situ observation of the cake by confocal laser scanning microscopy (CLSM) was designed, developed and validated. This device will be used in future studies to characterize cake structure at the microscopic scale.
References
[1]
Mota, M.; Teixeira, J.A.; Yelshin, A. Influence of cell-shape on the cake resistance in dead-end and cross-flow filtrations. Sep. Purif. Technol. 2002, 27, 137–144, doi:10.1016/S1383-5866(01)00202-7.
[2]
Fillaudeau, L.; Carrère, H. Yeast cells, beer composition and mean pore diameter impacts on fouling and retention during cross-flow filtration of beer with ceramic membranes. J. Membr. Sci. 2002, 196, 39–57, doi:10.1016/S0376-7388(01)00568-3.
[3]
Daufin, G.; Escudier, J.P.; Carrère, H.; Berot, S.; Fillaudeau, L.; Decloux, M. Recent and emerging applications of membrane processes in food and dairy industry. Food Bioprod. Process. 2001, 79, 89–102, doi:10.1205/096030801750286131.
[4]
Jin, J.; Chhatre, S.; Titchener-Hooker, N.J.; Bracewell, D.G. Evaluation of the impact of lipid fouling during the chromatographic purification of virus-like particles from Saccharomyces cerevisiae. J. Chem. Technol. Biotechnol. 2009, 85, 209–215.
[5]
Chau, S.; Baldascini, H.; Hearle, D.; Hoare, M.; Titchener-Hooker, N.J. Effect of fouling on the capacity and breakthrough characteristics of a packed bed ion exchange chromatography column. Bioprocess. Biosys. Eng. 2006, 28, 405–414, doi:10.1007/s00449-006-0046-3.
[6]
Wang, L.; Wang, X.; Fukushi, K. Effects of operational conditions on ultrafiltration membrane fouling. Desalination 2008, 229, 181–191, doi:10.1016/j.desal.2007.08.018.
[7]
Pelegrine, D.H.G.; Gasparetto, C.A. Whey proteins solubility as function of temperature and pH. Food Sci. Technol. 2005, 38, 77–80.
[8]
Nigam, M.O.; Bansal, B.; Dong, C.X. Fouling and cleaning of whey protein concentrate fouled ultrafiltration membranes. Desalination 2008, 218, 313–322, doi:10.1016/j.desal.2007.02.027.
[9]
Foley, G. A review of factors affecting filter cake properties in dead-end microfiltration of microbial suspensions. J. Membr. Sci. 2006, 274, 38–46, doi:10.1016/j.memsci.2005.12.008.
[10]
Foley, G.; McLoughlin, P.F.; Malone, D.M. Membrane fouling during constant flux crossflow microfiltration of dilute suspensions of active dry yeast. Sep. Sci. Technol. 1995, 30, 383–398, doi:10.1080/01496399508013878.
[11]
Iskra, T.; Bolton, G.R.; Coffman, J.L.; Godavarti, R. The effect of protein A cycle number on the performance and lifetime of an anion exchange polishing step. Biotechnol. Bioeng. 2013, 110, 1142–1152, doi:10.1002/bit.24781.
[12]
Corbett, R.; Carta, G.; Iskra, T.; Gallo, C.; Godavarti, R.; Salm, J.R. Structure and protein adsorption mechanisms of clean and fouled tentacle-type anion exchangers used in a monoclonal antibody polishing step. J. Chromatogr. A 2013, 1278, 116–125, doi:10.1016/j.chroma.2013.01.006.
[13]
Kuberkar, V.T.; Davis, R.H. Effects of added yeast on protein transmission and flux in cross-flow membrane microfiltration. Biotechnol. Prog. 1999, 15, 472–479, doi:10.1021/bp990023l.
[14]
Ye, Y.; Chen, V. Reversibility of heterogeneous deposits formed from yeast and proteins during microfiltration. J. Membr. Sci. 2005, 265, 20–28, doi:10.1016/j.memsci.2005.04.031.
[15]
Güll, C.; Czekaj, P.; Davis, R.H. Microfiltration of protein mixtures and the effects of yeast on membrane fouling. J. Membr. Sci. 1999, 155, 113–122, doi:10.1016/S0376-7388(98)00305-6.
[16]
Kawakatsu, T.; Nakao, S.; Kimura, S. Macromolecule rejection with compressible and incompressible cake layer formed in crossflow microfiltration. J. Chem. Eng. Jpn. 1993, 26, 656–661, doi:10.1252/jcej.26.656.
[17]
Tanaka, T.; Kamimura, R.; Fujiwara, R.; Nakanishi, K. Crossflow filtration of yeast broth cultivated in molasses. Biotechnol. Bioeng. 1994, 43, 1094–1101, doi:10.1002/bit.260431113.
[18]
Li, Y.; Shahbazi, A.; Kadzere, C.T. Separation of cells and proteins from fermentation broth using ultrafiltration. J. Food Eng. 2006, 75, 574–580, doi:10.1016/j.jfoodeng.2005.04.045.
[19]
Li, S.L.; Chou, K.S.; Lin, J.Y.; Yen, H.W.; Chu, I.M. Study on the microfiltration of Escherichia coli containing fermentation broth by a ceramic membrane filter. J. Membr. Sci. 1996, 110, 203–210, doi:10.1016/0376-7388(95)00250-2.
[20]
Okamoto, Y.; Ohmori, K.; Glatz, C.E. Harvest time effects on membrane cake resistance of Escherichia coli broth. J. Membr. Sci. 2001, 190, 93–106, doi:10.1016/S0376-7388(01)00431-8.
[21]
Boissier, B.; Lutin, F.; Moutounet, M.; Vernhet, A. Particles deposition during the cross-flow microfiltration of red wines-incidence of the hydrodynamic conditions and the yeast to fines ratio. Chem. Eng. Process. Process Intensif. 2008, 47, 276–286, doi:10.1016/j.cep.2007.01.027.
[22]
Mccarthy, A.A.; Walsh, P.K.; Foley, G. Experimental techniques for quantifying the cake mass, the cake and membrane resistances and the specific cake resistance during crossflow filtration of microbial suspensions. J. Membr. Sci. 2002, 201, 31–45, doi:10.1016/S0376-7388(01)00691-3.
[23]
Beaufort, S.; Alfenore, S.; Lafforgue, C. Use of fluorescent microorganisms to perform in vivo and in situ local characterization of microbial deposits. J. Membr. Sci. 2011, 369, 30–39, doi:10.1016/j.memsci.2010.11.023.
[24]
Hwang, B.-K.; Lee, C.-H.; Chang, I.-S.; Drews, A.; Field, R. Membrane bioreactor: TMP rise and characterization of bio-cake structure using CLSM-image analysis. J. Membr. Sci. 2012, 419–420, 33–41, doi:10.1016/j.memsci.2012.06.031.
[25]
Sun, C.; Fiksdal, L.; Hanssen-Bauer, A.; Rye, M.B.; Leiknes, T. Characterization of membrane biofouling at different operating conditions (flux) in drinking water treatment using confocal laser scanning microscopy (CLSM) and image analysis. J. Membr. Sci. 2011, 382, 194–201, doi:10.1016/j.memsci.2011.08.010.
[26]
Günther, J.; Schmitz, P.; Albasi, C.; Lafforgue, C. A numerical approach to study the impact of packing density on fluid flow distribution in hollow fiber module. J. Membr. Sci. 2010, 348, 277–286, doi:10.1016/j.memsci.2009.11.011.
[27]
Dufreche, J.; Prat, M.; Schmitz, P.; Sherwood, J.D. On the apparent permeability of a porous layer backed by a perforated plate. Chem. Eng. Sci. 2002, 57, 2933–2944, doi:10.1016/S0009-2509(02)00173-2.
[28]
Gassara, D.; Schmitz, P.; Ayadi, A.; Prat, M. Modelling the effect of particle size in microfiltration. Sep. Sci. Technol. 2008, 43, 1754–1770, doi:10.1080/01496390801973755.
[29]
Kimura, K.; Hane, Y.; Watanabe, Y.; Amy, G.; Ohkuma, N. Irreversible membrane fouling during ultrafiltration of surface water. Water Res. 2004, 38, 3431–3441, doi:10.1016/j.watres.2004.05.007.
[30]
Kumar, M.S.; Madhu, G.M.; Roy, S. Fouling behaviour, regeneration options and on-line control of biomass-based power plant effluents using microporous ceramic membranes. Sep. Purif. Technol. 2007, 57, 25–36, doi:10.1016/j.seppur.2007.03.002.
[31]
Image J Home Page. Available online: http://rsb.info.nih.gov/ij/ (accessed on 6 May 2013).
[32]
Russotti, G.; Osawa, A.E.; Sitrin, R.D.; Buckland, B.C.; Adams, W.R.; Lee, S.S. Pilot-scale harvest of recombinant yeast employing microfiltration: A case study. J. Biotechnol. 1995, 42, 235–246.
[33]
Kang, S.T.; Subramani, A.; Hoek, E.M.V.; Deshusses, M.A.; Matsumoto, M.R. Direct observation of biofouling in cross-flow microfiltration: Mechanisms of deposition and release. J. Membr. Sci. 2004, 244, 151–165, doi:10.1016/j.memsci.2004.07.011.
[34]
Stewart, P.S.; Robertson, C.R. Microbial growth in a fixed volume: Studies with entrapped Escherichia coli. Appl. Microbiol. Biotechnol. 1989, 30, 34–40.
[35]
McDonogh, R.M.; Fane, A.G.; Fell, C.J.D.; Flemming, H. The influence of polydispersity on the hydraulic behaviour of colloidal fouling layers on membranes perturbations on the behavior of the “ideal” colloidal layer. Colloids Surf. A. 1998, 138, 231–244.
[36]
Lebleu, N.; Roques, C.; Aimar, P.; Causserand, C. Role of the cell-wall structure in the retention of bacteria by microfiltration membranes. J. Membr. Sci. 2009, 326, 178–185, doi:10.1016/j.memsci.2008.09.049.
[37]
Johnson, W.P.; Martin, M.J.; Gross, M.J.; Logan, B.E. Facilitation of bacterial transport through porous media by changes in solution and surface properties. Colloids Surf. A 1996, 107, 263–271.
[38]
Sharma, M.M.; Chang, Y.I.; Yen, T.F. Reversible and irreversible surface charge modification of bacteria for facilitating transport through porous media. Colloids Surf. 1985, 16, 193–206, doi:10.1016/0166-6622(85)80252-3.
[39]
Mille, Y.; Beney, L.; Gervais, P. Viabilty of Escherichia coli after combined osmotic and thermal treatment: A plasma membrane implication. Biochim. Biophys. Acta 2002, 1567, 41–48, doi:10.1016/S0005-2736(02)00565-5.
[40]
Suchecka, T.; Piatkiewicz, W.; Sosnowski, T.R. Is the cell retention by MF membrane absolutely safe—A hypothetical model for cell deformation in a membrane pore. J. Membr. Sci. 2005, 250, 135–140, doi:10.1016/j.memsci.2004.08.035.
