The Scribble protein complex genes, consisting of lethal giant larvae ( Lgl), discs large ( Dlg) and scribble ( Scrib) genes, are components of an evolutionarily conserved genetic pathway that links the cell polarity in cells of humans and Drosophila. The tissue expression and developmental changes of the Scribble protein complex genes were documented using qRT-RCR method. The Lgl and Scrib genes could be detected in all the experimental tissues, including fat body, midgut, testis/ovary, wingdisc, trachea, malpighian tubule, hemolymph, prothoracic gland and silk gland. The Dlg gene, mainly expressed only in testis/ovary, could not be detected in prothoracic gland and hemolymph. In fat body, there were two higher expression stages of the three genes. The highest peak of the expression of the Lgl and Scrib genes in wingdisc lay at the 1st day of the 5th instar, but the Dlg gene was at 3rd day of 5th instar. The above results indicate that Scribble complex genes are involved in the process of molting and development of the wingdisc in the silkworm. This will be useful in the future for the elucidation of the detailed biological function of the three genes Scrib, Dlg and Lgl in B. mori.
References
[1]
Elsum, I.; Yates, L.; Humbert, P.O.; Richardson, H.E. The Scribble-Dlg-Lgl polarity module in development and cancer: From flies to man. Essays Biochem. 2012, 53, 141–168, doi:10.1042/bse0530141.
[2]
Assémat, E.; Bazellières, E.; Pallesi-Pocachard, E.; Le Bivic, A.; Massey-Harroche, D. Polarity complex proteins. BBA Biomembranes 2008, 1778, 614–630, doi:10.1016/j.bbamem.2007.08.029.
[3]
Bissell, M.J.; Radisky, D. Putting tumours in context. Nat. Rev. Cancer 2001, 1, 46–54, doi:10.1038/35094059.
[4]
Humbert, P.; Russell, S.; Richardson, H. Dlg, Scribble and Lgl in cell polarity, cell proliferation and cancer. Bioessays 2003, 25, 542–553, doi:10.1002/bies.10286.
[5]
Bilder, D. Epithelial polarity and proliferation control: Links from the Drosophila neoplastic tumor suppressors. Gene Dev. 2004, 18, 1909–1925, doi:10.1101/gad.1211604.
[6]
Brumby, A.M.; Richardson, H.E. Scribble mutants cooperate with oncogenic Ras or Notch to cause neoplastic overgrowth in Drosophila. EMBO J. 2003, 22, 5769–5779, doi:10.1093/emboj/cdg548.
[7]
Bilder, D.; Perrimon, N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 2000, 403, 676–680, doi:10.1038/35001108.
[8]
Woods, D.F.; Bryant, P.J. Molecular cloning of the lethal (1) discs large-1 oncogene of Drosophila. Dev. Biol. 1989, 134, 222–235, doi:10.1016/0012-1606(89)90092-4.
[9]
Enomoto, M.; Igaki, T. Deciphering tumor-suppressor signaling in flies: Genetic link between Scribble/Dlg/Lgl and the Hippo pathway. J. Genet. Genomics 2011, 38, 461–470, doi:10.1016/j.jgg.2011.09.005.
[10]
Jacob, L.; Opper, M.; Metzroth, B.; Phannavong, B.; Mechler, B.M. Structure of the l (2) gl gene of Drosophila and delimitation of its tumor suppressor domain. Cell 1987, 50, 215–225, doi:10.1016/0092-8674(87)90217-0.
[11]
Thomas, U.; Phannavong, B.; Müller, B.; Garner, C.C.; Gundelfinger, E.D. Functional expression of rat synapse-associated proteins SAP97 and SAP102 in Drosophila dlg-1 mutants: Effects on tumor suppression and synaptic bouton structure. Mech. Dev. 1997, 62, 161–174, doi:10.1016/S0925-4773(97)00658-8.
[12]
International Silkworm Genome Consortium. The genome of a lepidopteran model insect, the silkworm Bombyx mori. Insect Biochem. Mol. Biol. 2008, 38, 1036–1045.
[13]
Wei, Z.J.; Yu, M.; Tang, S.M.; Yi, Y.Z.; Hong, G.Y.; Jiang, S.T. Transcriptional regulation of the gene for prothoracicotropic hormone in the silkworm, Bombyx mori. Mol. Biol. Rep. 2011, 38, 1121–1127, doi:10.1007/s11033-010-0209-9.
[14]
Xia, H.; Zhang, C.; Feng, F.; Yuan, Y.; Zhou, Y.; Liu, X.; Chen, K. Molecular cloning, expression and characterization of a novel vacuolar protein sorting 4 gene in silkworm, Bombyx mori. Mol. Biol. Rep. 2012, 39, 10339–10346, doi:10.1007/s11033-012-1911-6.
[15]
Tang, S.; Wu, J.; Zhao, X.; Wang, H.; Qiu, Z.; Shen, X.; Guo, X. Molecular cloning and characterization of hatching enzyme-like geneII (BmHEL II) in the silkworm Bombyx mori. Biochem. Biophys. Res. Commun. 2012, 419, 194–199, doi:10.1016/j.bbrc.2012.01.144.
[16]
Tian, L.; Guo, E.; Diao, Y.; Zhou, S.; Peng, Q.; Cao, Y.; Li, S. Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body. BMC Genomics 2010, 11, 549, doi:10.1186/1471-2164-11-549.
Kl？mbt, C.; Schmidt, O. Developmental expression and tissue distribution of the lethal (2) giant larvae protein of Drosophila melanogaster. EMBO J. 1986, 5, 2955–2961.
