A biofuel cell (BFC) cathode has been developed based on direct electron transfer (DET) of hemoglobin (Hb) molecules with an indium-tin-oxide (ITO) electrode and their electrocatalysis for reduction of hydrogen peroxide (H 2O 2). In this study, the ITO-coated glass plates or porous glasses were prepared by using a chemical vapor deposition (CVD) method and examined the electrochemical characteristics of the formed ITO in pH 7.4 of phosphate buffered saline (PBS) solutions containing and not containing Hb. In half-cell measurements, the reduction current of H 2O 2 due to the electrocatalytic activity of Hb increased with decreasing electrode potential from around 0.1 V v ers us Ag|AgCl|KCl(satd.) in the PBS solution. The practical open-circuit voltage (OCV) on BFCs utilizing H 2O 2 reduction at the Hb-ITO cathode with a hydrogen (H 2) oxidation anode at a platinum (Pt) electrode was expected to be at least 0.74 V from the theoretical H 2 oxidation potential of ？0.64 V versus Ag|AgCl|KCl(satd.) in pH 7.4. The assembled single cell using the ITO-coated glass plate showed the OCV of 0.72 V and the maximum power density of 3.1 μW cm ？2. The maximum power per single cell was recorded at 21.5 μW by using the ITO-coated porous glass.
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