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Diversity  2013 

Preliminary Analysis of Life within a Former Subglacial Lake Sediment in Antarctica

DOI: 10.3390/d5030680

Keywords: Antarctica, bacteria, biodiversity, lake, polar, subglacial, sediment

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Abstract:

Since the first descriptions of Antarctic subglacial lakes, there has been a growing interest and awareness of the possibility that life will exist and potentially thrive in these unique and little known environments. The unusual combination of selection pressures, and isolation from the rest of the biosphere, might have led to novel adaptations and physiology not seen before, or indeed to the potential discovery of relic populations that may have become extinct elsewhere. Here we report the first microbiological analysis of a sample taken from a former subglacial lake sediment in Antarctica (Lake Hodgson, on the Antarctic Peninsula). This is one of a number of subglacial lakes just emerging at the margins of the Antarctic ice sheet due to the renewed onset of deglaciation. Microbial diversity was divided into 23.8% Actinobacteria, 21.6% Proteobacteria, 20.2% Planctomycetes and 11.6% Chloroflexi, characteristic of a range of habitat types ( Overall, common sequences were neither distinctly polar, low temperature, freshwater nor marine). Twenty three percent of this diversity could only be identified to “unidentified bacterium”. Clearly these are diverse ecosystems with enormous potential.

 References

[1]  Robin, G.D.Q.; Swithinbank, C.W.M.; Smith, M.B.E. Radio echo exploration of the Antarctic ice sheet. In International Symposium on Antarctic Glaciological Exploration (ISAGE); International Association of Scientific Hydrology: Hanover, NH, USA, 1970. Publication number 86; pp. 97–115.
[2]  Oswald, G.K.A.; Robin, G.D.Q. Lakes beneath Antarctic ice sheet. Nature 1973, 245, 251–254, doi:10.1038/245251a0.
[3]  Siegert, M.J.; Tranter, M.; Ellis-Evans, J.C.; Priscu, J.C.; Lyons, W.B. The hydrochemistry of lake Vostok and the potential for life in Antarctic subglacial lakes. Hydrol. Process. 2003, 17, 795–814, doi:10.1002/hyp.1166.
[4]  Siegert, M.J.; Carter, S.; Tabacco, I.; Popov, S.; Blankenship, D.D. A revised inventory of Antarctic subglacial lakes. Antarct. Sci. 2005, 17, 453–460, doi:10.1017/S0954102005002889.
[5]  Bell, R.E.; Studinger, M.; Shuman, C.A.; Fahnestock, M.A.; Joughin, I. Large subglacial lakes in East Antarctica at the onset of fast-flowing ice streams. Nature 2007, 445, 904–907, doi:10.1038/nature05554.
[6]  Jones, N. Russians celebrate vostok victory. Nature 2012, doi:10.1038/482287a.
[7]  Siegert, M.J.; Clarke, R.J.; Mowlem, M.; Ross, N.; Hill, C.S.; Tait, A.; Hodgson, D.; Parnell, J.; Tranter, M.; Pearce, D.; et al. Clean access, measurement, and sampling of Ellsworth subglacial lake: A method for exploring deep Antarctic subglacial lake environments. Rev. Geophys. 2012, 50, RG1003, doi:10.1029/2011RG000361.
[8]  Lake Ellsworth Consortium. Exploration of Ellsworth subglacial lake: A concept paper on the development, organisation and execution of an experiment to explore, measure and sample the environment of a West Antarctic subglacial lake. Rev. Env. Sci. Biotechnol. 2007, 6, 1569–1705.
[9]  Pearce, D.A. Antarctic subglacial lake exploration: A new frontier in microbial ecology. ISME J. 2009, 3, 877–880, doi:10.1038/ismej.2009.53.
[10]  Fricker, H.A.; Powell, R.; Priscu, J.; Tulaczyk, S.; Anandakrishnan, S.; Christner, B.; Fisher, A.T.; Holland, D.; Horgan, H.; Jacobel, R.; et al. Siple coast subglacial aquatic environments: The whillans ice stream subglacial access research drilling project. Geophys. Monogr. Ser. 2011, 194, 199–219.
[11]  Hodgson, D.A.; Roberts, S.J.; Bentley, M.J.; Smith, J.A.; Johnson, J.S.; Verleyen, E.; Vyverman, W.; Hodson, A.J.; Leng, M.J.; Cziferszky, A.; et al. Exploring former subglacial Hodgson Lake, Antarctica paper i: Site description, geomorphology and limnology. Quat. Sci. Rev. 2009, 28, 2295–2309, doi:10.1016/j.quascirev.2009.04.011.
[12]  Schutte, U.M.; Abdo, Z.; Bent, S.J.; Williams, C.J.; Schneider, G.M.; Solheim, B.; Forney, L.J. Bacterial succession in a glacier foreland of the high Arctic. ISME J. 2009, 3, 1258–1268, doi:10.1038/ismej.2009.71.
[13]  Anesio, A.M.; Hodson, A.J.; Fritz, A.; Psenner, R.; Sattler, B. High microbial activity on glaciers: Importance to the global carbon cycle. Glob. Chang. Biol. 2009, 15, 955–960, doi:10.1111/j.1365-2486.2008.01758.x.
[14]  Gaidos, E.; Lanoil, B.; Thorsteinsson, T.; Graham, A.; Skidmore, M.; Han, S.K.; Rust, T.; Popp, B. A viable microbial community in a subglacial volcanic crater lake, Iceland. Astrobiology 2004, 4, 327–344, doi:10.1089/ast.2004.4.327.
[15]  Miteva, V.I.; Brenchley, J.E. Detection and isolation of ultrasmall microorganisms from a 120,000-year-old Greenland glacier ice core. Appl. Environ. Microbiol. 2005, 71, 7806–7818, doi:10.1128/AEM.71.12.7806-7818.2005.
[16]  Abyzov, S.S.; Hoover, R.B.; Mitskevich, I.N.; Mulyukin, A.L.; Poglazova, M.N.; Rozanov, A.Y. Microbiological Methodology in Astrobiology. Microbial extremophiles: psychrophiles i. In Astrobiology and Planetary Missions, Proceedings of the SPIE 2005, San Diego, CA, USA, 31 July 2005.
[17]  Gaidos, E.; Marteinsson, V.; Thorsteinsson, T.; Jóhannesson, T.; Rúnarsson, A.; Stefansson, A.; Glazer, B.; Lanoil, B.; Skidmore, M.; Han, S.; et al. An oligarchic microbial assemblage in the anoxic bottom waters of a volcanic subglacial lake. ISME J. 2009, 3, 486–497, doi:10.1038/ismej.2008.124.
[18]  Marteinsson, V.T.; Rúnarsson, á.; Stefánsson, A.; Thorsteinsson, T.; Jóhannesson, T.; Magnússon, S.H.; Reynisson, E.; Einarsson, B.; Wade, N.; Morrison, H.G.; et al. Microbial communities in the subglacial waters of the Vatnaj？kull ice cap, Iceland. ISME J. 2013, 7, 427–437, doi:10.1038/ismej.2012.97.
[19]  Christner, B.C.; Royston-Bishop, G.; Foreman, C.M.; Arnold, B.R.; Tranter, M.; Welch, K.A.; Berry Lyons, W.; Tsapin, A.I.; Studinger, M.; Priscu, J.C. Limnological conditions in subglacial Lake Vostok, Antarctica. Limnol. Oceanogr. 2006, 51, 2485–2501, doi:10.4319/lo.2006.51.6.2485.
[20]  D’Elia, T.; Veerapaneni, R.; Rogers, S.O. Isolation of microbes from Lake Vostok accretion ice. Appl. Environ. Microbiol. 2008, 74, 4962–4965, doi:10.1128/AEM.02501-07.
[21]  D’Elia, T.; Veerapaneni, R.; Theraisnathan, V.; Rogers, S.O. Isolation of fungi from Lake Vostok accretion ice. Mycologia 2009, 101, 751–763, doi:10.3852/08-184.
[22]  Rogers, S.O.; Shtarkman, Y.M.S.; Ko？er, Z.A.; Edgar, R.; Veerapaneni, R.; D’Elia, T. Ecology of subglacial Lake Vostok (Antarctica), based on metagenomic/metatranscriptomic analyses of accretion ice. Biology 2013, 2, 629–650, doi:10.3390/biology2020629.
[23]  Skidmore, M. Microbial communities in Antarctic subglacial aquatic environments (SAE). In Exploration and Study of Antarctic Subglacial Aquatic Environments; Siegert, M.J., Kennicutt II, M.C., Bindschandler, R.A., Eds.; AGU Geophysical Monograph Series; ACU Press: Washington, DC, USA, 2011; Volume 192, pp. 61–81.
[24]  Bulat, S.A.; Alekhina, I.A.; Lipenkov, V.Y.; Lukin, V.V.; Marie, D.; Petit, J.R. Cell concentrations of microorganisms in glacial and lake ice of the vostok ice core, east antarctica. Microbiology 2009, 78, 808–810, doi:10.1134/S0026261709060216.
[25]  Priscu, J.C.; Adams, E.E.; Lyons, W.B.; Voytek, M.A.; Mogk, D.W.; Brown, R.L.; McKay, C.P.; Takacs, C.D.; Welch, K.A.; Wolf, C.F.; et al. Geomicrobiology of subglacial ice above Lake Vostok, Antarctica. Science 1999, 286, 2141–2144, doi:10.1126/science.286.5447.2141.
[26]  Christner, B.C.; Mosley-Thompson, E.; Thompson, L.G.; Reeve, J.N. Isolation of bacteria and 16s RDNAs from Lake Vostok accretion ice. Environ. Microbiol. 2001, 3, 570–577, doi:10.1046/j.1462-2920.2001.00226.x.
[27]  Lanoil, B.; Skidmore, M.; Priscu, J.C.; Han, S.; Foo, W.; Vogel, S.W.; Tulaczyk, S.; Engelhardt, H. Bacteria beneath the West Antarctic ice sheet. Environ. Microbiol. 2009, 11, 609–615, doi:10.1111/j.1462-2920.2008.01831.x.
[28]  Hodgson, D.A.; Roberts, S.J.; Bentley, M.J.; Carmichael, E.L.; Smith, J.A.; Verleyen, E.; Vyverman, W.; Geissler, P.; Leng, M.J.; Sanderson, D.C.W. Exploring former subglacial Hodgson Lake, Antarctica. Paper ii: Palaeolimnology. Quat. Sci. Rev. 2009, 28, 2310–2325, doi:10.1016/j.quascirev.2009.04.014.
[29]  Porter, K.G.; Feig, Y.S. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 1980, 25, 943–948, doi:10.4319/lo.1980.25.5.0943.
[30]  Amann, R.I.; Binder, B.J.; Olson, R.J.; Chisholm, S.W.; Devereux, R.; Stahl, D.A. Combination of 16S RNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 1990, 56, 1919–1925.
[31]  Stahl, D.A. Development and application of nucleic acid probes. In Nucleic Acid Techniques in Bacterial Systematics; Stackebrandt, E., Ed.; John Wiley & Sons Ltd.: Chichester, UK, 1991; pp. 205–248.
[32]  Manz, W.; Amann, R.; Ludwig, W.; Wagner, M.; Schleifer, K.H. Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria-problems and solutions. Syst. Appl. Microbiol. 1992, 15, 593–600, doi:10.1016/S0723-2020(11)80121-9.
[33]  Glockner, F.O.; Fuchs, B.M.; Amann, R. Bacterioplankton compositions of lakes and oceans: A first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol. 1999, 65, 3721–3726.
[34]  Manz, W.; Amann, R.; Ludwig, W.; Vancanneyt, M.; Schleifer, K.H. Application of a suite of 16S RNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacter-Bacteroides in the natural environment. Microbiol. UK 1996, 142, 1097–1106, doi:10.1099/13500872-142-5-1097.
[35]  Boetius, A.; Ravenschlag, K.; Schubert, C.J.; Rickert, D.; Widdel, F.; Gieseke, A.; Amann, R.; Jorgensen, B.B.; Witte, U.; Pfannkuche, O. A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 2000, 407, 623–626, doi:10.1038/35036572.
[36]  Glockner, F.O.; Zaichikov, E.; Belkova, N.; Denissova, L.; Pernthaler, J.; Pernthaler, A.; Amann, R. Comparative 16S RNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of Actinobacteria. Appl. Environ. Microbiol. 2000, 66, 5053–5065, doi:10.1128/AEM.66.11.5053-5065.2000.
[37]  Meier, H.; Amann, R.; Ludwig, W.; Schleifer, K.H. Specific oligonucleotide probes for in situ detection of a major group of Gram-positive bacteria with low DNA G + C content. Syst. Appl. Microbiol. 1999, 22, 186–196, doi:10.1016/S0723-2020(99)80065-4.
[38]  Wallner, G.; Amann, R.; Beisker, W. Optimizing fluorescent in situ hybridization with RNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 1993, 14, 136–143, doi:10.1002/cyto.990140205.
[39]  Herrera, A.; Cockell, C.S.; Self, S.; Blaxter, M.; Reitner, J.; Arp, G.; Drose, W.; Thorsteinsson, T.; Tindle, A.G. Bacterial colonization and weathering of terrestrial obsidian in Iceland. Geomicrobiol. J. 2008, 25, 25–37, doi:10.1080/01490450701828982.
[40]  Cockell, C.S.; Olsson, K.; Knowles, F.; Kelly, L.; Herrera, A.; Thorsteinsson, T.; Marteinsson, V. Bacteria in weathered basaltic glass, iceland. Geomicrobiol. J. 2009, 26, 491–507, doi:10.1080/01490450903061101.
[41]  Mergeay, M.; Nies, D.; Schlegel, H.G.; Gerits, J.; Charles, P.; van Gijsegem, F. Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals. J. Bacteriol. 1985, 162, 328–334.
[42]  Ryan, F.J. Selected methods of neurospora genetics. Methods Med. Res. 1950, 3, 51–75.
[43]  Geneious homepage. Available online: http://www.geneious.com (accessed on 1 July 2013).
[44]  Schloss, P.D.; Gevers, D.; Westcott, S.L. Reducing the effects of PCR amplification and sequencing artifacts on 16S RNA-based studies. PloS One 2011, 6, e27310, doi:10.1371/journal.pone.0027310.
[45]  Gomez-Alvarez, V.; Teal, T.K.; Schmidt, T.M. Systematic artifacts in metagenomes from complex microbial communities. ISME J. 2009, 3, 1314–1317, doi:10.1038/ismej.2009.72.
[46]  Meyer, F.; Paarmann, D.; D’Souza, M.; Olson, R.; Glass, E.M.; Kubal, M.; Paczian, T.; Rodriguez, A.; Stevens, R.; Wilke, A.; et al. The metagenomics rast server-a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinforma. 2008, 9, 386:1–386:8.
[47]  Pruesse, E.; Quast, C.; Knittel, K.; Fuchs, B.M.; Ludwig, W.; Peplies, J.; Glockner, F.O. Silva: A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res. 2007, 35, 7188–7196, doi:10.1093/nar/gkm864.
[48]  DeSantis, T.Z.; Hugenholtz, P.; Larsen, N.; Rojas, M.; Brodie, E.L.; Keller, K.; Huber, T.; Dalevi, D.; Hu, P.; Andersen, G.L. Greengenes, a chimera-checked 16S RNA gene database and workbench compatible with arb. Appl. Environ. Microbiol. 2006, 72, 5069–5072, doi:10.1128/AEM.03006-05.
[49]  Cole, J.R.; Wang, Q.; Cardenas, E.; Fish, J.; Chai, B.; Farris, R.J.; Kulam-Syed-Mohideen, A.S.; McGarrell, D.M.; Marsh, T.; Garrity, G.M.; et al. The ribosomal database project: Improved alignments and new tools for RNA analysis. Nucleic Acids Res. 2009, 37, D141–D145, doi:10.1093/nar/gkn879.
[50]  Chao, A. Nonparametric-estimation of the number of classes in a population. Scand. J. Stat. 1984, 11, 265–270.
[51]  Dong, H.; Zhang, G.; Jiang, H.; Yu, B.; Chapman, L.R.; Lucas, C.R.; Fields, M.W. Microbial diversity in sediments of saline Qinghai Lake, China: Linking geochemical controls to microbial ecology. Microb. Ecol. 2006, 51, 65–82, doi:10.1007/s00248-005-0228-6.
[52]  Jiang, H.; Dong, H.; Zhang, G.; Yu, B.; Chapman, L.R.; Fields, M.W. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in Northwestern China. Appl. Environ. Microbiol. 2006, 72, 3832–3845, doi:10.1128/AEM.02869-05.
[53]  Mancuso, C.A.; Franzmann, P.D.; Burton, H.R.; Nichols, P.D. Microbial community structure and biomass estimates of a methanogenic Antarctic lake ecosystem as determined by phospholipid analyses. Microb. Ecol. 1990, 19, 73–95, doi:10.1007/BF02015055.
[54]  Glockner, F.O.; Kube, M.; Bauer, M.; Teeling, H.; Lombardot, T.; Ludwig, W.; Gade, D.; Beck, A.; Borzym, K.; Heitmann, K.; et al. Complete genome sequence of the marine planctomycete Pirellula sp. Strain 1. Proc. Natl. Acad. Sci. USA 2003, 100, 8298–8303, doi:10.1073/pnas.1431443100.
[55]  Breznak, J.A.; Canale-Parola, E. Spirochaeta aurantia, a pigmented, facultatively anaerobic spirochete. J. Bacteriol. 1969, 97, 386–395.
[56]  Monciardini, P.; Cavaletti, L.; Schumann, P.; Rohde, M.; Donadio, S. Conexibacter woesei gen. Nov., sp. Nov., a novel representative of a deep evolutionary line of descent within the class actinobacteria. Int. J. Syst. Evol. Microbiol. 2003, 53, 569–576, doi:10.1099/ijs.0.02400-0.
[57]  Aksenova, H.Y.; Rainey, F.A.; Janssen, P.H.; Zavarzin, G.A.; Morgan, H.W. Spirochaeta-thermophila sp-nov, an obligately anaerobic, polysaccharolytic, extremely thermophilic bacterium. Int. J. Syst. Bacteriol. 1992, 42, 175–177, doi:10.1099/00207713-42-1-175.
[58]  Botero, L.M.; Brown, K.B.; Brumefield, S.; Burr, M.; Castenholz, R.W.; Young, M.; McDermott, T.R. Thermobaculum terrenum gen. nov., sp. nov.: A non-phototrophic gram-positive thermophile representing an environmental clone group related to the chloroflexi (green non-sulfur bacteria) and thermomicrobia. Arch. Microbiol. 2004, 181, 269–277, doi:10.1007/s00203-004-0647-7.
[59]  Tyson, G.W.; Lo, I.; Baker, B.J.; Allen, E.E.; Hugenholtz, P.; Banfield, J.F. Genome-directed isolation of the key nitrogen fixer Leptospirillum ferrodiazotrophum sp. nov. From an acidophilic microbial community. Appl. Environ. Microbiol. 2005, 71, 6319–6324, doi:10.1128/AEM.71.10.6319-6324.2005.
[60]  Maymo-Gatell, X.; Anguish, T.; Zinder, S.H. Reductive dechlorination of chlorinated ethenes and 1, 2-dichloroethane by “Dehalococcoides ethenogenes” 195. Appl. Environ. Microbiol. 1999, 65, 3108–3113.
[61]  Ward, N.; Larsen, O.; Sakwa, J.; Bruseth, L.; Khouri, H.; Durkin, A.S.; Dimitrov, G.; Jiang, L.; Scanlan, D.; Kang, K.H.; et al. Genomic insights into methanotrophy: The complete genome sequence of Methylococcus capsulatus (bath). PLoS Biol. 2004, 2, e303, doi:10.1371/journal.pbio.0020303.
[62]  Shivaji, S.; Kumari, K.; Kishore, K.H.; Pindi, P.K.; Rao, P.S.; Radha Srinivas, T.N.; Asthana, R.; Ravindra, R. Vertical distribution of bacteria in a lake sediment from antarctica by culture-independent and culture-dependent approaches. Res. Microbiol. 2011, 162, 191–203, doi:10.1016/j.resmic.2010.09.020.
[63]  Sjoling, S.; Cowan, D.A. High 16S RDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica. Extremophiles 2003, 7, 275–282, doi:10.1007/s00792-003-0321-z.
[64]  Brambilla, E.; Hippe, H.; Hagelstein, A.; Tindall, B.J.; Stackebrandt, E. 16S RDNAdiversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, Mcmurdo Dry Valleys, Antarctica. Extremophiles 2001, 5, 23–33, doi:10.1007/s007920000169.
[65]  Sutherland, D.L.; Hawes, I. Annual growth layers as proxies of past growth conditions for benthic microbial mats in a perennially ice-covered Antarctic lake. FEMS Microbiol. Ecol. 2009, 67, 279–292, doi:10.1111/j.1574-6941.2008.00621.x.
[66]  Li, S.; Xiao, X.; Yin, X.; Wang, F. Bacterial community along a historic lake sediment core of Ardley Island, West Antarctica. Extremophiles 2006, 10, 461–467, doi:10.1007/s00792-006-0523-2.
[67]  Bowman, J.P.; McCammon, S.A.; Gibson, J.A.; Robertson, L.; Nichols, P.D. Prokaryotic metabolic activity and community structure in Antarctic continental shelf sediments. Appl. Environ. Microbiol. 2003, 69, 2448–2462, doi:10.1128/AEM.69.5.2448-2462.2003.
[68]  Stackebrandt, E.; Brambilla, E.; Cousin, S.; Dirks, W.; Pukall, R. Culture-independent analysis of bacterial species from an anaerobic mat from Lake Fryxell, Antarctica: Prokaryotic diversity revisited. Cell. Mol. Biol. 2004, 50, 517–524.
[69]  Purdy, K.J.; Nedwell, D.B.; Embley, T.M. Analysis of the sulfate-reducing bacterial and methanogenic archaeal populations in contrasting Antarctic sediments. Appl. Environ. Microbiol. 2003, 69, 3181–3191, doi:10.1128/AEM.69.6.3181-3191.2003.
[70]  Bowman, J.P.; Rea, S.M.; McCammon, S.A.; McMeekin, T.A. Diversity and community structure within anoxic sediment from marine salinity meromictic lakes and a coastal meromictic marine basin, Vestfold Hills, eastern Antarctica. Environ. Microbiol. 2000, 2, 227–237, doi:10.1046/j.1462-2920.2000.00097.x.
[71]  Chen, M.; Xiao, X.; Wang, P.; Zeng, X.; Wang, F. Arthrobacter ardleyensis sp. nov., isolated from Antarctic Lake sediment and deep-sea sediment. Arch. Microbiol. 2005, 183, 301–305, doi:10.1007/s00203-005-0772-y.
[72]  Wang, Z.W.; Liu, Y.H.; Dai, X.; Wang, B.J.; Jiang, C.Y.; Liu, S.J. Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int. J. Syst. Evol. Microbiol. 2006, 56, 439–442, doi:10.1099/ijs.0.64065-0.
[73]  Karr, E.A.; Ng, J.M.; Belchik, S.M.; Sattley, W.M.; Madigan, M.T.; Achenbach, L.A. Biodiversity of methanogenic and other archaea in the permanently frozen Lake Fryxell, Antarctica. Appl. Environ. Microbiol. 2006, 72, 1663–1666, doi:10.1128/AEM.72.2.1663-1666.2006.
[74]  Farmer, J.D.; Warner, N.H. Subglacial hydrothermal alteration minerals in j？kulhlaup deposits of southern iceland, with implications for detecting past or present habitable environments on Mars. Astrobiology 2010, 10, 523–547, doi:10.1089/ast.2009.0425.
[75]  Bjornsson, H. Subglacial lakes and jokulhlaups in Iceland. Glob. Planet. Change 2002, 35, 255–271, doi:10.1016/S0921-8181(02)00130-3.
[76]  Garchar, L.; Wendlandt, R.; Martini, B.; Owens, L. Geochemistry of a sub glacial volcanic hydrothermal system at Mount Spurr, Alaska. In Proceedings of Thirty Seventh Workshop on Geothermal Reservoir Engineering Stanford, Stanford, CA, USA, 30 January–1 February 2012.
[77]  Petit, J.R.; Alekhina, I.A.; Bulat, S.A. A hydrothermal contribution to the Vostok subglacial lake (Antarctica) suggested from bacterial gene analysis and the stable isotope composition of deep ice core samples. In Proceedings of 35th COSPAR Scientific Assembly, Paris, France, 18–25 July 2004; p. 832.
[78]  Lavire, C.; Normand, P.; Alekhina, I.; Bulat, S.; Prieur, D.; Birrien, J.-L.; Fournier, P.; H？nni, C.; Petit, J.-R. Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox. Environ. Microbiol. 2006, 8, 2106–2114, doi:10.1111/j.1462-2920.2006.01087.x.
[79]  Parkes, R.J.; Cragg, B.A.; Wellsbury, P. Recent studies on bacterial populations and processes in subseafloor sediments: A review. Hydrogeol. J. 2000, 8, 11–28, doi:10.1007/PL00010971.
[80]  Park, B.-J.; Park, S.-J.; Yoon, D.-N.; Schouten, S.; Sinninghe Damsté, J.S.; Rhee, S.-K. Cultivation of autotrophic ammonia-oxidizing archaea from marine sediments in coculture with sulfur-oxidizing bacteria. Appl. Environ. Microbiol. 2010, 76, 7575–7587, doi:10.1128/AEM.01478-10.
[81]  Mathis, B.J.; Marshall, C.W.; Milliken, C.E.; Makkar, R.S.; Creager, S.E.; May, H.D. Electricity generation by thermophilic microorganisms from marine sediment. Appl. Microbiol. Biotechnol. 2008, 78, 147–155, doi:10.1007/s00253-007-1266-4.
[82]  Li, H.; Yu, Y.; Luo, W.; Zeng, Y.; Chen, B. Bacterial diversity in surface sediments from the Pacific Arctic Ocean. Extremophiles 2009, 13, 233–246, doi:10.1007/s00792-009-0225-7.

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