The objectives of this study were to chemically characterize and evaluate the antioxidant activity of essential oils Cinnamodendron dinisii Schwacke (pepper) and Siparuna guianensis Aublet (negramina). The essential oil was isolated by hydrodistillation using a Clevenger modified apparatus, and the identification and quantification of constituents, through GC/MS and GC-FID analysis. The antioxidant activity was evaluated using β-carotene/linoleic acid system and the DPPH radical sequestering method. In chromatographic analysis, the majority constituents found in the essential oil of C. dinisii were bicyclic monoterpenes, α-pinene (35.41%), β-pinene (17.81%), sabinene (12.01%) and sesquiterpene bicyclogermacrene (7.59%). In the essential oil of the fresh leaves of Siparuna guianensis Aublet, acyclic monoterpene, β-myrcene (13.14%), and sesquiterpenes, germacrene-D (8.68%) and bicyclogermacrene (16.71%) were identified. The antioxidant activity was low by the β-carotene/linoleic acid test and was not evidenced by the DPPH test, for both oils evaluated.
References
[1]
Sim？es, C.M.O.; Schenkel, E.P.; Gosmann, G.; Mello, J.C.P.; Mentz, L.A.; Petrovick, P.R. Farmacognosia: da Planta ao medicamento, 6th ed. ed.; UFSC/UFRGS: Porto Alegre, Brazil, 2007; p. 1104.
[2]
Bandoni, A.L.; Czepak, M.P. Os Recursos Vegetais Aromáticos no Brasil: Seu Aproveitamento Industrial Para a Produ？？o de Aromas e Sabores, 1st ed. ed.; EDUFES: Vitória, Brazil, 2008; pp. 345–367.
[3]
Bizzo, H.; Hovell, A.M.C.; Rezende, C.M. óleos essenciais no Brasil: Aspectos gerais, desenvolvimento e perspectivas. Quím. Nova 2009, 32, 588–594.
[4]
Ramalho, V.C.; Jorge, N. Antioxidantes utilizados em óleos, gorduras e alimentos gordurosos. Quím. Nova 2006, 29, 755–760.
[5]
Lima, E.S.; Abdalla, D.S.P. Peroxida？？o lipídica: Mecanismos e avalia？？o em amostras biológicas. Revista Bras. Cienc. Farm. 2001, 37, 293–303.
Yunes, R.A.; Cechinel Filho, V. Química de Produtos Naturais, Novosfármacos e a Moderna Farmacognosia, 2nd ed. ed.; Univale Editora: Itajaí, Brasil, 2009; pp. 219–256.
[8]
Lorenzi, H. árvores Brasileiras: Manual de Identifica？？o e Cultivo de Plantas Arbóreas do Brasil, 4th ed. ed.; Editora Instituto Plantarum: Nova Odessa, Brazil, 2002; Volume 2, pp. 175–177.
[9]
Assis, L.; Peixoto, A.L.; de Barros, F. Canellaceae in Lista de Espécies da Flora do Brasil. Jardim Botanico do Rio de Janeiro, 2010. Available online: http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB111724 (accessed on 12 January 2013).
[10]
Valentini, C.M.A.; Silva, L.E.; Maciel, E.N.; Franceschini, E.; Sousa, P.T., Jr.; Dall’Oglio, E.L.; Coelho, M.F.B. Varia？？o anual do rendimento e composi？？o química dos componentes voláteis da Siparuna guianensis Aublet. Quím. Nova 2010, 33, 1506–1509.
[11]
Agência Nacional de Vigilancia Sanitária. Farmacopeia Brasileira, 5th ed.; Anvisa: Brasília, Brazil, 2010; pp. 198–199.
[12]
Guimar？es, L.G.L.; Cardoso, M.G.; Zacaroni, L.M.; Lima, R.K.; Pimentel, F.A.; Morais, A.R. Influência da luz e da temperatura sobre a oxida？？o do óleo essencial de capim-lim？o (Cymbopogon citratus (D.C.) Stapf). Quím. Nova 2008, 31, 1476–1480.
[13]
Mendes, M.D.; Lima, A.S.; Trindade, H.; Correia, A.I.D.; Barroso, J.G.; Pedro, L.G.; Figueiredo, A.C. ISSR molecular characterization and leaf volatiles analysis of Pittosporum undulatum Vent. naturalized in the Azores archipelago (Portugal). Ind. Crops Prod. 2011, 33, 710–719, doi:10.1016/j.indcrop.2011.01.010.
[14]
Tepe, B.; Daferera, D.; Sokmen, A.; Sokmen, M.; Polissiou, M. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem. 2005, 90, 333–340.
[15]
Lopes-Lutz, D.; Alviano, D.S.; Alviano, C.S.; Kolodziejczyk, P.P. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 2008, 69, 1732–1738, doi:10.1016/j.phytochem.2008.02.014.
[16]
Wang, W.; Wu, N.; Zu, Y.G.; Fu, Y.J. Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. Food Chem. 2008, 108, 1019–1022, doi:10.1016/j.foodchem.2007.11.046.
[17]
Ferreira, D.F. SISVAR: um Programa para análises e ensino de estatística. Revista Symp. 2008, 6, 36–41.
[18]
Torres, E.; Wisniewski, A., Jr.; Simionatto, E.L. Composi？？o química dos componentes voláteis de Capsicodendron dinisii Schwancke (Canellaceae). Quím. Nova 2010, 33, 130–132.
[19]
Adams, R.P.; Zanoni, T.A. Essential oil of plants from Hispaniola: 1- The volatile wood oil of Cinnamodendron ekamani (Canellaceae). Moscosoa 1989, 5, 154–158.
[20]
Tucker, A.O.; Maciarello, M.J.; Brown, R.; Griffeth, M. A commercial essential oil of Mandravasarotra (Cinnamosma fragrans Baill., Canellaceae) from Madagascar. J. Essent. Oil Res. 2008, 20, 259–260, doi:10.1080/10412905.2008.9700006.
[21]
Setzer, W.N. Chemical composition of the leaf essential oil of Canella winterana from Abaco Island, Bahamas. J. Essent. OilBear. Plants 2007, 10, 475–479, doi:10.1080/0972060X.2007.10643581.
[22]
Amiguet, T.V.; Petit, P.; Ta, C.A.; Nu？ez, R.; Sánchez-Vindas, P.; Alvarez, L.P.; Smith, M.L.; Arnason, J.T.; Durst, T. Phytochemistry and antifungal properties of the newly discovered tree Pleodendron costaricense. J. Nat. Prod. 2006, 69, 1005–1009, doi:10.1021/np0504863.
Antonio, T.M.; Waller, G.R.; Mussinan, C.J. Composition of essential oil from the leaves of Siparuna guianensis (Monimiaceae). Chem. Ind. 1984, 14, 514–515.
[25]
Rebou？as, L.M.C. Terpenos de Siparuna guianensis: Aldeídos via Epoxida？？o de Duplas terminais. Master’s Thesis, Universidade Federal do Ceará, Fortaleza, Brazil, 17 February 1984.
[26]
Montanari, R.M. Composi？？o Química e Atividades Biológicas dos óleos Essenciais de Espécies de Anacardiaceae, Siparunaceae e Verbenaceae. Ph.D. Thesis, Universidade Federal de Vi？osa, Vi？osa, Brazil, 20 December 2010.
[27]
Fischer, D.C.H.; Limberger, R.P.; Henriques, A.T.; Moreno, P.R.H. Essential oils from fruits and leaves of Siparuna guianensis (Aubl.) Tulasne from southeastern Brazil. J. Essent. Oil Res. 2005, 17, 101–102, doi:10.1080/10412905.2005.9698844.
Gobbo-Neto, L.; Lopes, N.P. Plantas medicinais: Fatores de influência no conteúdo de metabólitos secundários. Quím. Nova 2007, 30, 374–381, doi:10.1590/S0100-40422007000200026.
Kulisic, T.; Radonic, A.; Katalinic, V.; Milos, M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem. 2004, 85, 633–640.
[32]
Ruberto, G.; Baratta, M.T. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem. 2000, 69, 167–174, doi:10.1016/S0308-8146(99)00247-2.
[33]
Mata, A.T.; Proen？a, C.; Ferreira, A.R.; Serralheiro, M.L.M.; Nogueira, J.M.F.; Araújo, M.E.M. Antioxidant and antiacetylcholinesterase activities of five plants used as portuguese food spices. Food Chem. 2007, 103, 778–786, doi:10.1016/j.foodchem.2006.09.017.
[34]
Viuda-Martos, M.; Navajas, Y.R.; Zapata, E.S.; Fernández-López, J.; Pérez-álvarez, J.A. Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet. Flavour Fragr. J. 2009, 25, 13–19.
[35]
Guimar？es, L.G.L. óleos Essenciais de Lippia sidoides Cham., Alomia fastigiata (Gardner) Benth, Ocotea odorifera (Vell.) Rohwer, Mikania glauca Mart. e Cordia verbenacea D.C.: Identifica？？o e Quantifica？？o Química, Caracteriza？？o das Estruturas Secretoras, Atividades Antioxidante e Antibacteriana. Ph.D. Thesis, Universidade Federal de Lavras, Lavras, Brazil, 11 June 2010.
[36]
Lima, R.K.; Cardoso, M.G.; Andrade, M.A.; Guimar？es, P.L.; Batista, L.R.; Nelson, D.L. Bactericidal and antioxidant activity of essential oils from Myristica fragrans Houtt and Salvia microphylla H.B.K. J. Am. Oil Chem. Soc. 2012, 89, 523–528, doi:10.1007/s11746-011-1938-1.
