Simultaneous Estimation of Pantoprazole Sodium and Levosulpiride in Capsule Dosage Form by Simultaneous Equation Spectrophotometric Method

A simple, accurate, and precise spectrophotometric method was developed for simultaneous estimation of pantoprazole sodium and levosulpiride in capsule dosage form. Simultaneous equation was developed at 290 and 232？nm. The method was found to be linear in the range of 4–12？μg/mL for pantoprazole sodium and 8–20？μg/mL for levosulpiride while accuracy of the method was confirmed by recovery studies of capsule dosage form and was found to be 100.23–100.99% and 100.51–100.94% for pantoprazole sodium and levosulpiride, respectively, in their capsule dosage form. The proposed method was validated and found to be accurate and precise. The method was successfully applied for the estimation of pantoprazole sodium and levosulpiride from their capsule dosage form. 1. Introduction Pantoprazole sodium (PNT) is a sodium 5-(difluoromethoxy)-2-[[(3,4-dimethoxy]-2-pyridinyl) methyl]sulfinyl]-1H-benzimidazole [1] (Figure 1); it is a proton pump inhibitor drug used for short-term treatment of erosion and ulceration of the esophagus caused by gastroesophageal reflux disease [2]. It has molecular formula C16H14F2N3NaO4S with molar mass 405.36？g/mol [1]. Levosulpiride (LVS) is an N-[(1-ethyl-2-pyrrolidinyl)methyl]-2-methoxy-5-sulfamoylbenzamide (Figure 2); it is a new orally effective antipsychotic and a prokinetic agent reported to be a selective antagonist of dopamine D2 receptor activity on both central and peripheral levels. It is an atypical neuroleptic (S)-enantiomer of sulpiride having molecular formula C15H23N3O4S with molar mass 341.43？g/mol. Levosulpiride is also claimed to have mood elevating property and used in the treatment of psychoses [3], particularly negative symptoms of schizophrenia, anxiety disorders, dysthymia, vertigo, dyspepsia, irritable bowel syndrome, and premature ejaculation [4]. Figure 1: Structure of pantoprazole sodium. Figure 2: Structure of levosulpiride. The literature review revealed that LC methods [5, 6] have been reported for the estimation of PNT. Various RP-LC methods have been reported for the estimation of PNT in combination with domperidone [7] and naproxen [8, 9], respectively. Derivative spectrophotometric method has been reported for the estimation of LVS in tablet dosage form [10]. Simultaneous estimation of LVS and esomeprazole by UV spectrometry has been reported [11]. LC method has been reported for estimation of LVS from human plasma [12]. LC method have been reported for the estimation of LVS in combination with esomeprazole [13] and rabeprazole [14, 15], respectively. RP-LC method has been reported for the estimation of