A simple and highly selective spectrophotometric method has been developed for the determination of selegiline hydrochloride in bulk and formulations. Method A is based on the oxidation of 3-methyl-2-benzothiazolinone hydrazone in the presence of ceric ammonium sulphate, followed by its coupling reaction with drug to form a colored product having of 629？nm. Method B is based on the coupling reaction of drug with 4-aminoantipyrine to give a new ligand that reacts with copper(II) to give intense bluish red colored chelate which is measured at 539？nm. Beer’s law is obeyed in the range of 10.00–85.00？μg？mL？1 with molar absorptivity of for method A and 20.00–120.00？μg？mL？1 with molar absorptivity of for method B. The optimum reaction condition and the analytical parameters are evaluated. The results obtained indicate that the methods are free from interference of the ingredients; thus they are successfully applied to pharmaceutical formulations. 1. Introduction Selegiline hydrochloride (SGE) (Figure 1) (deprenyl), [(R)-(2)-N-methyl-(1-phenyl-2-propyl)-N-propinylamine] hydrochloride [1], is a levomethamphetamine derivative which belongs to a class of drugs called phenethylamines [2]. SEG is a selective, irreversible inhibitor of monoamine oxidase (MAO-A) [3]. It is used for the treatment of early-stage Parkinson’s disease, depression, and senile dementia [4]. It is useful adjunct in the treatment of cocaine addiction [5]. SEG (brand name Anipryl) is also used (at extremely high dosages relative to humans) in veterinary medicine to treat the symptoms of Cushing’s disease and cognitive dysfunction (canine cognitive dysfunction) in dogs [6]. Recommended dosage of SEG is about 10？mg/day; further increase in the dosage will lead to the nonselective inhibition of MAO [7]. Therefore, it is essential to develop a standard analytical method for monitoring residual drug in bulk and in pharmaceutical formulations. The pharmaceutical importance of drug has prompted us to devise methods for the rapid determination of SEG. A very few methods are reported in the literature for the determination of SEG in pharmaceutical formulations which include high performance liquid chromatography [8–12], gas chromatography [13, 14], fluorescence polarization immunoassay (FPIA) and gas chromatography-mass spectrometry (GC/MS) [15], spectrofluorometry [16], and stereoselective analyses [17]. The literature survey reveals that so far there is no visible spectrophotometric method for the analysis of SEG. Figure 1: Chemical structure of SEG. Unlike gas chromatograph and high performance
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