全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

DOI: 10.1155/2013/293935

Full-Text   Cite this paper   Add to My Lib

Abstract:

High performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer was used for separation and identification of phenolic and other compounds in the water extracts of Saraca asoca (Roxb.), De. Wilde. The aim of the study was to identify and evaluate the distribution of phenolic compounds in the different parts of the plant. The identity of compounds was established through the comparison with standards and characteristic base peaks as well as other daughter ions. In crude extracts, 34 catechin derivatives, 34 flavonoids, and 17 other compounds were identified. Interestingly, further analysis of compounds showed plant part specific unique pattern of metabolites; that is, regenerated bark is observed to be the best source for catechin/catechin derivative while flowers were found to be the source for wide variety of flavonoids. Moreover, these plant part specific compounds can be used as biomarkers for the identification of plant material or herbal drugs. Overall, the present study provides for the first time a comprehensive analysis of the phenolic components of this herb which may be helpful not only to understand their usage but also to contribute to quality control as well. 1. Introduction Bark decoction of S. asoca (Roxb.), De. Wilde (Caesalpiniaceae), has been mentioned as one of the most famous Indian treatise Charaka Samhita (100?A.D.) for the treatment of various types of gynaecological disorders. Bhavprakash Nighantu, another Indian treatise, referred to it as a uterine tonic for regularizing the menstrual disorders. Bark of the plant is well reported for its stimulating effect on endometrium and ovarian tissues and being used to treat menorrhagia. S. asoca contains significant amounts of phenolic compounds that are considered to be the biologically active components. Water extracts of the plant parts are being used to prepare various Ayurvedic and herbal drugs being rich source of catechin, epicatechin, epigallocathechin, and their polymers and glucosides [1, 2]. Catechins are well reported for various kinds of biological activities and are useful for the symptomatic treatment of several gastrointestinal, respiratory, and vascular diseases. The antioxidant activity of flavonoids has been studied with regard to retarding the aging of cells and protection against cancer and coronary or cardiovascular disease [3–5]. Various techniques are in use to identify phenolic compounds such as thin layer chromatography, high performance thin layer chromatography, gas chromatography, UV detection, high performance liquid

References

[1]  A. Gahlaut, P. Taneja, A. Shirolkar, A. Nale, V. Hooda, and R. Dabur, “Principal component and partial least square discriminant based analysis of methanol extracts of bark and regenerated bark of Saraca asoca,” International Journal of Pharmacy and Pharmaceutical Sciences, vol. 4, no. 4, pp. 331–335, 2012.
[2]  J. Saha, T. Mitra, K. Gupta, and S. Mukherjee, “Phytoconstituents and HPTLC analysis in Saraca asoca (Roxb.) Wilde,” International Journal of Pharmacy and Pharmaceutical Sciences, vol. 4, no. 1, pp. 96–99, 2012.
[3]  R. L. Galli, B. Shukitt-Hale, K. A. Youdim, and J. A. Joseph, “Fruit polyphenolics and brain aging,” Annals of the New York Academy of Sciences, vol. 959, pp. 128–132, 2002.
[4]  R. Zamora-Ros, C. Not, E. Guinó et al., “Association between habitual dietary flavonoid and lignan intake and colorectal cancer in a Spanish case—control study (the Bellvitge Colorectal Cancer Study),” Cancer Causes & Control, vol. 24, no. 3, pp. 549–557, 2013.
[5]  L. Testai, A. Martelli, M. Cristofaro, M. C. Breschi, and V. Calderone, “Cardioprotective effects of different flavonoids against myocardial ischaemia/reperfusion injury in Langendorff-perfused rat hearts,” Journal of Pharmacy and Pharmacology, vol. 65, no. 5, pp. 750–756, 2013.
[6]  A. Chaudhary, P. Kaur, N. Kumar, B. Singh, S. Awasthi, and B. Lal, “Chemical fingerprint analysis of phenolics of Albizia chinensis based on ultra-performance LC-electrospray ionization-quadrupole time of flight mass spectrometry and antioxidant activity,” Natural Product Communications, vol. 6, no. 11, pp. 1617–1620, 2011.
[7]  A. Shirolkar, A. Gahlaut, V. Hooda, and R. Dabur, “Phytochemical composition changes in untreated stem juice of Tinospora cordifolia (W) Mier during refrigerated storage,” Journal of Pharmacy Research, vol. 7, no. 1, pp. 1–6, 2013.
[8]  J. M. Cevallos-Cevallos, J. I. Reyes-De-Corcuera, E. Etxeberria, M. D. Danyluk, and G. E. Rodrick, “Metabolomic analysis in food science: a review,” Trends in Food Science & Technology, vol. 20, no. 11-12, pp. 557–566, 2009.
[9]  R. Goodacre, S. Vaidyanathan, W. B. Dunn, G. G. Harrigan, and D. B. Kell, “Metabolomics by numbers: acquiring and understanding global metabolite data,” Trends in Biotechnology, vol. 22, no. 5, pp. 245–252, 2004.
[10]  A. Shirolkar, A. Gahlaut, A. K. Chhillar, and R. Dabur, “Quantitative analysis of catechins in Saraca asoca and correlation with antimicrobial activity,” Journal of Pharmaceutical Analysis, 2013.
[11]  D. Shen, Q. Wu, M. Wang, Y. Yang, E. J. Lavoie, and J. E. Simon, “Determination of the predominant catechins in Acacia catechu by liquid chromatography/electrospray ionization-mass spectrometry,” Journal of Agricultural and Food Chemistry, vol. 54, no. 9, pp. 3219–3224, 2006.
[12]  Y. Sun, X. Zhang, X. Xue, Y. Zhang, H. Xiao, and X. Liang, “Rapid identification of polyphenol C-glycosides from Swertia franchetiana by HPLC-ESI-MS-MS,” Journal of Chromatographic Science, vol. 47, no. 3, pp. 190–196, 2009.
[13]  M. Careri, L. Elviri, and A. Mangia, “Validation of a liquid chromatography ion spray mass spectrometry method for the analysis of flavanones, flavones and flavonols,” Rapid Communication in Mass Spectrometry, vol. 13, no. 23, pp. 2399–2405, 2009.
[14]  P. J. Madeira, C. M. Borges, and M. H. Florêncio, “Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometric and semi-empirical calculations study of five isoflavone aglycones,” Rapid Communications in Mass Spectrometry, vol. 24, no. 23, pp. 3432–3440, 2010.
[15]  G. C. Justino, C. Borges, and M. H. Florêncio, “Electrospray ionization tandem mass spectrometry fragmentation of protonated flavone and flavonol aglycones: a re-examination,” Rapid Communications in Mass Spectrometry, vol. 23, no. 2, pp. 237–248, 2009.
[16]  http://spectra.psc.riken.jp.
[17]  http://www.massbank.jp.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133