In this paper, a new formulation of nasal spray was set up based on the extract of lemon pulp, obtained by using a new solid-liquid technology of extraction, added to pure Aloe juice, soluble propoli, and essential oils of Ravensara and Niaouly. It was tested in a clinical study in which 100 subjects were recruited for a period of one month. Nasal scraping was used for collecting samples and after the application of the May-Grünwald Giemsa standard technique, glass slides were analysed by using optical microscope with a 1000x oil immersion. A control group constituted of ten people was recruited as control and this group was administered with physiological solution (saline solution). The comparison of results obtained before and after the application of nasal spray showed a total reduction of eosinophils granulocytes and mast cells; clinical data were confirmed by improvement of clinical pictures of patients. The lemon-based nasal spray was a good alternative to conventional medicine for the treatment of perennial and seasonal allergic and vasomotor rhinopathy. 1. Nasal Cytology Nasal cytology is of remarkable importance in the study of rhinosinus diseases, especially the vasomotor rhinitis (VMR), as it represents valuable means of differential diagnosis between allergic/nonallergic diseases and bacterial/viral infections. It is a popular and proven method, considering that it dates back to 1889, when Gollash [1] identified the numerous eosinophils in the nasal secretion from an asthmatic patient and attributed their presence a key role in the pathogenesis of asthma. The nasal cytodiagnosis was actually encouraged by the study of Eyermann [2] in 1927, who identified the eosinophils in the nasal exudate of allergic patients and underlined its diagnostic importance. Since then, lots of researchers have focused their attention on cytology and particularly on the presence of different types of inflammatory cells in nasal diseases [3, 4]. Different factors have contributed to the increased interest in cytological study of the nasal mucosa, making this procedure more widespread: the sampling is easy to perform and minimally invasive, allowing the examination to be repeated, as often required in the follow-up visits in the case of vasomotor disorders and for monitoring the effectiveness of some treatments. Some studies have proved that the rhinocytogram of patients with allergies varies according to the topical nasal steroid treatment. Meltzer et al. [5] and Small [6] have shown that fluticasone dipropionate and beclomethasone dipropionate are able to
References
[1]
H. Gollash, “Zur kenntnis des asthmatischen sputums,” Fortschritte der Medizin, vol. 7, pp. 361–365, 1889.
[2]
Ch. Eyermann, “Nasal manifestation of allergy,” Annals of Otology, vol. 5, pp. 357–366, 1927.
[3]
F. K. Hansel, “Observations on the cytology of the secretions in allergy of the nose and paranasal sinuses,” Journal of Allergy, vol. 5, no. 4, pp. 357–366, 1934.
[4]
P. A. T. Bogaerts and P. A. R. Clement, “The diagnostic value of a cytogram in rhinopathology,” Rhinology, vol. 19, no. 4, pp. 203–208, 1981.
[5]
E. O. Meltzer, A. Jalowasyki, and E. Meltzer, “Correlation between nasal cytogram and blow technique for the diagnosis of allergic rhinitis,” Annals of Allergy, vol. 66, pp. 86–88, 1991.
[6]
P. Small, “Beclomethasone dipropionate nasal aerosol in adult patients with ragweed seasonal rhinitis,” Annals of Allergy, vol. 49, no. 1, pp. 20–22, 1982.
[7]
P. Cassano, M. Gelardi, R. Ricco, A. Cimmino, and M. Cassano, “Variations of cells of immuno-phlogosis in chronic rhinopathies, based on the treatment with anti-histamine pharamaceuticals and topical corticosteroids,” Giornale Italiano di Allergologia e Immunologia Clinica, vol. 12, pp. 94–100, 2002.
[8]
D. Naviglio, “Naviglio's principle and presentation of an innovative solid-liquid extraction technology: extractor Naviglio,” Analytical Letters, vol. 36, no. 8, pp. 1647–1659, 2003.
[9]
D. Naviglio, F. Pizzolongo, R. Romano, L. Ferrara, and A. Santini, “An innovative solid-liquid extraction technology: use of the Naviglio Extractor for the production of lemon liquor,” African Journal of Food Science, vol. 1, pp. 42–50, 2007.
[10]
M. Gelardi, M. L. Fiorella, C. Russo, R. Fiorella, and G. Ciprandi, “Role of nasal cytology,” International Journal of Immunopathology and Pharmacology, vol. 23, no. 1, pp. 45–49, 2010.
[11]
D. Naviglio, T. Caruso, P. Iannece, A. Aragòn, and A. Santini, “Characterization of high purity lycopene from tomato wastes using a new pressurized extraction approach,” Journal of Agricultural and Food Chemistry, vol. 56, no. 15, pp. 6227–6231, 2008.
[12]
J. Degen, M. Seiberling, I. Meyer, P. Thomann, and T. Schürholz, “Influence of a nasal spray consisting of a standardized composition of Citrus limon (succus) and an aqueous extract from Cydonia oblonga (fructus) on the intranasal mucociliary clearance,” Arzneimittel-Forschung/Drug Research, vol. 50, no. 1, pp. 39–42, 2000.
[13]
C. Caristi, E. Bellocco, V. Panzera, G. Toscano, R. Vadalà, and U. Leuzzi, “Flavonoids detection by HPLC-DAD-MS-MS in lemon juices from Sicilian cultivars,” Journal of Agricultural and Food Chemistry, vol. 51, no. 12, pp. 3528–3534, 2003.
[14]
O. Benavente-García, J. Castillo, F. R. Marin, A. Ortu？o, and J. A. Del Río, “Uses and properties of citrus flavonoids,” Journal of Agricultural and Food Chemistry, vol. 45, no. 12, pp. 4505–4515, 1997.
[15]
I. V. Alekseeva, “Intranasal use of tincture of aloe in atrophic nasopharyngitis and,” Vestnik Otorinolaringologii, vol. 18, no. 1, pp. 22–24, 1956.
[16]
J. E. Thompson, “Topical use of aloe vera derived allantoin gel in otolaryngology,” Ear, Nose and Throat Journal, vol. 70, no. 2, p. 119, 1991.
[17]
A. R. Ramanoelina, G. P. Terrom, J. P. Bianchini, and P. Coulanges, “Antibacterial action of essential oils extracted from Madagascar plants,” Archives de l'Institut Pasteur de Madagascar, vol. 53, no. 1, pp. 217–226, 1987.
[18]
A. Bellanger, M. H. Becquemin, D. Feldman, J. F. Bertholon, and F. Tankere, “Optimization of aerosol therapy in otorhinolaryngology: stability and granulometry of dexamethasone-gomenol-framycetin solution,” Annales d'Oto-Laryngologie et de Chirurgie Cervico-Faciale, vol. 118, no. 1, pp. 45–53, 2001.
[19]
A. J. G. H. Kostermans, “Le genre Ravensara Sonn. (Lauracées) a Madagascar,” Bulletin du Jardin Botanique de l'état, vol. 28, no. 2, pp. 173–191, 1958.
[20]
F. R. Gallo and G. Savi, “Propolis: its use in technology and research,” Bollettino Chimico Farmaceutico, vol. 134, no. 9, pp. 483–491, 1995.
[21]
S. Sosa, A. Bornancin, A. Tubaro, and R. Della Loggia, “Topical antiinflammatory activity of an innovative aqueous formulation of Actichelated Propolis vs two commercial propolis formulations,” Phytotherapy Research, vol. 21, no. 9, pp. 823–826, 2007.
[22]
M. Gelardi, A. Maselli Del Giudice, T. Candreva et al., “Nasal resistance and allergic inflammation depend on allergen type,” International Archives of Allergy and Immunology, vol. 141, no. 4, pp. 384–389, 2006.
[23]
G. Cordone, A. Orlandini, and L. Dore, “Diagnostic importance of the rhinocytogram in chronic nrhinopathies,” Russian River Aud Blonde, vol. 1, pp. 18–21, 1996.
[24]
G. Angel-Solano and R. Shuturnan, “Comparative cytology of nasal secretion and nasal eosinophilia in seasonal allergic rhinitis,” Annals of Allergy, vol. 56, pp. 521–525, 1986.
[25]
J. T. Bickmore, “Nasal cytology in allergy and infection,” Otolaryngologic Allergy, vol. 40, pp. 39–46, 1978.
[26]
G. H. Caughey, “Mast cell proteases as protective and inflammatory mediators,” Advances in Experimental Medicine and Biology, vol. 716, pp. 212–234, 2011.
