We report the biosynthesis of silver nanoparticles (AgNPs) in a single step using edible fruit aqueous extract of P. peruviana that essentially involved the concept of green chemistry. Yellowish-brown color appeared upon adding the broth of P. peruviana to aqueous solution of 1？mM AgNO3 which indicates the formation of AgNPs. The maximum synthesis of these nanoparticles was being achieved in nearly 2？hrs at 28°C. The synthesis of AgNPs was followed by AgNPs UV-visible spectroscopy. Particle size and morphology of AgNPs were studied by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), respectively. These studies revealed that the AgNPs characterized were spherical in shape with diameter ranging from 31 to 52？nm. The energy dispersive X-ray spectroscopy showed that the AgNPs present are approximately 63.42 percent by weight in the colloidal dispersion. The absorption spectra of the AgNPs in absence and presence of dl-alanine show a distinguish shift in surface plasmon resonance (SPR) bands. Thus, these nanoparticles may be used as a chemical sensor for dl-alanine present in the human blood. 1. Introduction Nanobiotechnology is the most emerging field in the recent time owing to many applications over other conventional techniques due to the diversity in nature and availability of more biologically processed components from plants for the formation of nanostructures. In the recent past, nanobiotechnology has acquired more recognition due to multidisciplinary approch and emerged as a novel technique used for various applications in different fields. The Self-dispersed, controlled shape and size of nanoparticles play a pivotal contribution in the field of environment, biotechnology, and biomedical applications. To synthesize metal nanoparticles, different approaches and methods have been exploited, namely, ultraviolet irradiation, aerosol technologies, lithography, laser ablation, ultrasonic fields, and photochemical reduction techniques reported in the literature. Since most of these procedures involve toxic and hazardous chemicals which render them expensive and environmentally unfriendly, therefore, an environment friendly and sustainable green chemistry approach will be highly appreciated to avoid the use of hazardous chemicals. The two approaches used to synthesize nanosized particles are top-down and bottom-up strategies. Nanoparticles as-synthesized are generally ≤100？nm in the dimension [1]. Along with many benefits, there are some drawbacks of chemically based synthesized nanosize particles. It involves the bulk use of
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