Introduction. The aim of this study was to investigate the effect of three different types of disinfectant agents on alginate impression material after 5 and 10 minutes. Method and Materials. In this in vitro experimental study, 66 circular samples of alginate impression material were contaminated with Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans fungus. Except for control samples, all of them were disinfected with sodium hypochlorite 0.525, Deconex, and Epimax by way of spraying. Afterwards, they were kept in plastic bags with humid rolled cotton for 5 and 10 minutes. The number of colonies was counted after 24 and 48 hours for bacteria and after 72 hours for fungus. Statistical Mann-Whitney test was used for data analysis (α = 0.05). Results. After 5 minutes, Epimax showed the highest disinfection action on Staphylococcus aureus as it completely eradicated the bacteria. The disinfection capacity of different agents can be increased as time elapses except for Pseudomonas aeruginosa which was eradicated completely in both 5 and 10 minutes. Conclusion. This study revealed that alginate can be effectively disinfected by three types of disinfecting agents by spraying method, although Epimax showed the highest disinfection action after 10 minutes compared to other agents. 1. Introduction Dentists, dental materials, and dental laboratories are exposed to different types of pathogenic microorganisms. Impression materials, impression trays, and poured stone cast have been said to be the main source of cross infection between patients and dentists [1]. New researches have shown that 67% of materials sent to dental laboratories are infected by various microorganisms [2]. The most frequently identified microorganisms are Streptococcus species, Staphylococcus species, Escherichia coli species, Actinomyces species, Antitratus species, Pseudomonas species, Enterobacter species, Klebsiella pneumonia, and Candida species [3]. Taking this into account, we should make an effort to eliminate most of these microorganisms and reduce the rate of infection transmission in dental laboratories. The International Dental Federation (IDF) insists on disinfecting all impressions taken from patients before sending them to laboratories [4]. Also the American Dental Association (ADA) has advised all dental stuffs to disinfect patients’ impression trays [5]. In some studies, it has been declared that washing the impression materials with tap water only removes 40% of bacteria; however, some studies reported that it has the capacity to reduce 90%, microorganisms
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