Response of six improved grass pea genotypes to prolonged salinity stress was investigated on seedlings grown in pot experiment using 150？mM NaCl up to 60 days of growth after commencement of treatment (DAC). NaCl exposure significantly reduced growth potential of varieties PUSA-90-2 and WBK-CB-14, but no such effect was observed in varieties B1, BioL-212 and in two mutant lines LR3 and LR4. A time-bound measurement at 15, 30 and 60 DAC revealed significant reduction in plant dry matter production, orchestrated through abnormally low capacity of leaf photosynthesis accompanied by low K+/Na+ ratio and onset of oxidative stress in all six genotypes at 15 DAC and the extension of the phenomena in PUSA-90-2 and WBK-CB-14 to 60 DAC. High superoxide dismutase (SOD) activity coupled with low ascorbate redox and declining ascorbate peroxidase (APX) and catalases (CAT) levels led to abnormal rise in H2O2 content at reproductive stage (30 DAC) in the latter two genotypes, consequently, resulting in NaCl-induced oxidative damage. H2O2 level in the rest of the four genotypes was modulated in a controlled way by balanced action of SOD, APX and CAT, preventing oxidative damage even under prolonged NaCl-exposure. Enzyme isoforms were involved in regulation of foliar H2O2-metabolism, which was critical in determining As tolerance of grass pea genotypes. 1. Introduction Soil salinity is one of the most severe abiotic stresses affecting production of the crops worldwide [1, 2]. This problem is more severe in arid and semiarid regions, and legume plants already face a notable impact of salt stress in these regions [3, 4]. The legume family is the second only to the cereals in their importance to mankind [3], but unfortunately, improvements of this group of plants for their tolerance against soil salinity stress have not kept pace with those of cereals and oil seeds. Salinity induces oxidative stress through the generation of reactive oxygen species (ROS) within the plant cells [5]. The resultant damage is generally manifested by different alterations at cellular level including membrane lipid peroxidation, electrolyte leakage, and sometimes over accumulation of hydrogen peroxide (H2O2). H2O2 is a highly diffusible ROS within plant cell and its dual roles as a stress-inducer and at the same time as a signaling molecule to upregulate primary antioxidant defense during oxidative stress have been increasingly recognized in different crops including legumes [6–8]. Among the prominent enzymatic system involved in ROS scavenging, SOD constitutes the first line of defense, but it
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