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ISRN Agronomy  2012 

Electrical Conductivity Testing as Applied to the Assessment of Freshly Collected Kielmeyera coriacea Mart. Seeds

DOI: 10.5402/2012/378139

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Abstract:

Assessment of seed vigor has long been an important tool of seed quality control programs. The conductivity test is a promising method for assessment of seed vigor, but proper protocols for its execution have yet to be established. The objective of this study was to assess the efficiency of electrical conductivity (EC) testing as a means of assessing the viability of freshly collected Kielmeyera Coriacea Mart. seeds. The test was performed on individual seeds rather than in a bulk configuration. Seeds were soaked for different periods (30?min, 90?min, 120?min., 180?min, and 240?min) at a constant temperature of 25°C. Conductivity was then measured with a benchtop EC meter. 1. Introduction Seeds are the primary factor of the seedling production process, despite their minor contribution to the end cost of each seedling. In order to estimate the success rate of seedling production, it is essential that seed characteristics such as vigor and germinability be known [1]. The importance of knowing the characteristics of Brazilian forest species to safer and more objective management of seedling production cannot be overstated. However, such studies are scarce, particularly in light of the vast number of species with this potential [2]. Given the intensity of anthropogenic pressure and the importance of rehabilitating disrupted or degraded environments, in-depth research of forest species is warranted. Routine methods used for determination of seed quality and viability include germination testing and the tetrazolium test. Methods such as measurement of soak solution pH, electrical conductivity, and potassium content of leachate, all based on the permeability of the cell membrane system, are increasingly being employed in the assessment of seed vigor, as they are reliable and fast and can thus speed the decision making process. Electrical conductivity testing, as applied to forest seeds, has yet to be standardized. Studies conducted thus far have focused on assessment of seed soaking times, which may range from 4 to 48 hours. Even at 48 hours, the conductivity test is considered a rapid technique as compared to the germination test, which, despite its status as a widespread and firmly established method, can take anywhere from 30 to 360 days to yield results (depending on species), and is limited by factors such as dormant seeds. The total concentration of electrolytes leached by seeds during soaking has long been assessed indirectly, mostly through the conductivity test, which takes advantage of the fact that inorganic ions make up a substantial portion of

References

[1]  J. M. M. Matos, Evaluation of pH test on exudate check feasibility of forest seeds, dissertation, University of Brasília, Brasília, Brazil, 2009.
[2]  F. Poggiani, S. Bruni, and E. S. Q. Barbos, “Effect of shading on seedling growth of three species forest,” in National conference on native plants, vol. 2, pp. 564–569, Institute of Forestry, 1992.
[3]  M. B. Mcdonald Jr. and D. O. Wilson, “ASA-610 ability to detect changes in soybean seed quality,” Journal of Seed Technology, vol. 5, no. 1, pp. 56–66, 1980.
[4]  S. Matthews and A. Powell, “A eletrical conductivity test,” in Handbook of Vigor Test Methods, D. A. Perry, Ed., pp. 37–42, International Seed Testing Associaty, Zurich, Switzerland, 1981.
[5]  J. Son Mark, W. R. Singh, A. D. C. Novembre, and H. M. C. P. Chamma, “Comparative studies to evaluate dem'etodos physiological quality of soybean seeds, with emphasis the electrical conductivity test,” Brazilian Journal of Agricultural Research, vol. 25, no. 12, pp. 1805–1815, 1990.
[6]  S. R. Singh, A. P. Silva, C. B. Munhoz, et al., Guide of Cerrado Plants Used in the Chapada Veadeiros, WWF-Brazil, Brasilia, Brazil, 2001.
[7]  J. M. Felfili, C. W. Fagg, J. C. S. Silva, et al., Plants of the APA Gama Cabe?a de Veado: Species, ecosystems and recovery, University of Brasilia, Brasília, Department of Engineering Forest, Brasília, Brazil, 2002.
[8]  M. F. B. Muniz, et al., “Comparison of methods for evaluating the physiological and health quality of melon seeds,” Journal of Seeds, Pellets, vol. 26, no. 2, pp. 144–149, 2004.
[9]  D. C. F. S. Dias and J. Marcos Filho, “Electrical conductivity to assess seed vigor of soybean (Glycine max (L.) Merrill),” Scientia Agricola, vol. 53, no. 1, Article ID article id, pp. 31–42, 1996.
[10]  J. C. Delouche and C. C. Baskin, “Acelerated aging techniques for predicting the relative storability of seed lots,” Seed Science and Technology, vol. 1, no. 2, pp. 427–452, 1973.
[11]  R. D. Vieira and F. C. Krzyzanowski, “Electrical conductivity test,” in Seed Vigor: Concepts and Tests, F. C. Krzyzanowski, R. D. Vieira, and J. B. Fran?a Neto, Eds., pp. 4.1–4.26, Abrates, London, UK, 1999.
[12]  R. D. Vieira, “Electrical conductivity test,” in Seed Vigor Tests, R. D. Vieira and N. M. Carvalho, Eds., p. 103, FUNEP, Jaboticabal, Brazil, 1994.
[13]  A. S. Amaral and S. T. Peske, “Exudate pH to estimate, in 30 minutes seed viability of soybeans,” Journal of seeds, vol. 6, no. 3, pp. 85–92, 1984.
[14]  E. J. Fernandes, R. Sader, and N. M. Carvalho, “seed viability beans (Phaseolus vulgaris L.) estimated by the pH of the exudate,” in Congress Brazil's Seeds, Gramado, Brazil, 1987.
[15]  F. C. Krzyzanowski and R. D. Vieira, “Electrical conductivity test,” in Seed Vigor: Concepts and Tests, F. C. Krzyzanowski, R. D. Vieira, and J. B. France Neto, Eds., pp. 4.1–4.26, Abrates, London, UK, 1999.
[16]  Ministry of Agriculture, Livestock and Supply, Rule for seed testing, SNPA/DNPV/CLAV, Brasilia, Brazil, 1992.
[17]  Ministry of Agriculture, Livestock and Supply, Rule for seed testing, SNPA/DNPV/CLAV, Brasilia, Brazil, 2009.
[18]  N. M. Carvalho and J. Nakagawa, Seeds: Science, Technology and Production, FUNEP, Jaboticabal, Brazil, 2000.
[19]  Pina-Rodrigues, et al., “Quality test,” in Germination from Basic to Applied, A. Ferreira and G. F. Borghetti, Eds., pp. 283–297, 2004.
[20]  A. G. Ferreira and F. Borghetti, from basic to Germination applied, Artmed, Porto Alegre, Brazil, 2004.
[21]  L. G. Labouriau, seed germination, OAS, Washington, DC, USA, 1983.
[22]  L. L. Rodrigues, Study of imbibition time for application the method of electrical conductivity in the verification of the feasibility forest seeds stored, monograph, University of Brasília, Brasília, Brazil, 2010.
[23]  J. T. Melo, J. F. Ribeiro, and V. L. G. F. Lima, “Germination of Seeds of some tree species native to the Cerrado,” Journal of Seeds, vol. 1, no. 2, pp. 8–12, 1979.

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