The male reproductive system of fruit bat (Eidolon helvum) was studied histologically using light microscope. Thirty males (17 adults and 13 juveniles) were captured using net, weighed, aged using relative ossification of the wing bone, and dissected and reproductive tissue was processed for histomorphometry. On the basis of histological sections, the structures of a pair of testis containing the seminiferous tubules of adults were compacted in organization with spermatogenic cells. The epididymis has a thinner muscular region than the vas deferens with longitudinal folds on the mucosal lining. Two portions were observed in the prostate gland, while seminal vesicle has numerous trabeculae and bulbourethral gland was observed to have multiacini. There was increase in thickness of muscular region, epithelial height, and luminal diameter of epididymis and vas deferens between adults and juveniles. This work has documented the histology of the male reproductive system in bats, and ultrastructure and histochemistry are recommended for further insight into the reproductive biology. 1. Introduction Bats (Chiroptera) are among the most diverse and widely distributed groups of mammals and can be found in most continents [1]. There are around 1,150 species of bats. Bats are second only to rodents in numbers of living genera and species, which represents nearly a quarter of all the species of mammals on earth, and the majority live in tropical and semitropical regions [1]. Bats are the only flying mammals and they have a wide range of feeding and roosting habits, social behaviours, and reproductive strategies [2]. Diversity in their biology makes bats not only a fascinating group of animals to study but also a difficult one. They are unique in their agility (potential for long-distance travel) and often aggregate in very large colonies, and these activities aid in the spread of diseases [3]. They are natural reservoir for a large number of zoonotic pathogens such as rabies virus [4], coronavirus [5], and Henipavirus, that is, nipah virus, hendra virus [6], and possibly Ebola virus [7, 8]. Eidolon helvum are commonly found in moist and dry tropical rain forest, including evergreen forest habitats in the form of coastal (including mangrove) and riverine forest, through moist and dry savanna and mosaics of these and similar habitat types. Populations can persist in modified habitats and the species is often recorded in urban areas, such as wooded city parks [9]. Some works on the physiology, histology, breeding, and pathology of reproductive organs of the female bats
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