The purified fish visceral protease enzyme was immobilized by using various concentrations of sodium alginate and calcium chloride to optimize the best concentration for the formation of the beads. Then it was characterized by assaying the optimal pH, temperature, storage stability and reusability. The results on immobilization with sodium alginate and calcium chloride showed that a combination of 2% sodium alginate and 0.3？M calcium chloride weas found to be the optimum concentration for the formation of spherical and stable beads, this gave a maximal entrapped activity of 48.31%, and there was no change in the optimum pH 8.0 and temperature 40°C of protease before and after entrapment. The results on stability and reusability indicated that it was stable at 4°C retaining 100% residual activity after 5 days of storage and 67% loss of activity after ten days of storage and it retained 100% residual activity on the first reuse, 75% residual activity on the second reuse, 25% residual activity on the third use and complete loss in the activity on the fourth reuse. 1. Introduction Labeo rohita, commonly called Rohu, is a member of the family Cyprinidae within the order Cypriniformes [1]. It is a prime cultured and important staple fresh water fish generally found in rivers, ponds and reservoirs [2]. In recent years, recovery and characterization of enzymes such as alkaline phosphatase, hyaluronidase, acetylglucosaminidase, chitinase, and protease from fish have been carried out, and these have led to the emergence of some interesting new applications of these enzymes in food processing [3, 4]. Protease is an enzyme which hydrolyses proteins, that is, catabolizes proteins by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain forming the proteins. Proteases also known as peptidyl-peptide hydrolase constitute 60–65% of the global enzyme market [5, 6]. Proteases are mainly derived from animal, plant, and microbial sources. They play an essential role in the growth and survival of all living organisms. For marine animals, proteases are mainly produced by the digestive glands. The most important digestive enzymes are pepsin, secreted from gastric mucosa; trypsin and chymotrypsin secreted from the pancreas, pyloric caeca, and intestine. Like the proteases from plants, animals and microorganisms, digestive proteases from marine animals, are polyfunctional enzymes catalyzing the hydrolytic degradation of proteins [7]. The protease enzyme has diverse applications in a wide variety of industries such as detergent, food,
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