Background. T-box expressed in T cells (TBET) and guanine adenine thymine adenine sequence-binding protein 3 (GATA3) play important roles in the differentiation of Th1 and Th2 subsets, which contributes to the progression of acute coronary syndrome (ACS). Objective. This study aimed to investigate the temporal change of TBET/GATA3 mRNA ratio in ACS. Methods. Thirty-three patients suspected of ACS with symptom onset within 24 hours were recruited. Blood samples were taken after arrival at the emergency department and at hourly intervals until the 6th hour. The mRNA expressions of TBET and GATA3 were quantified by a real-time RT-qPCR. Results. The TBET/GATA3 mRNA ratio was elevated dramatically in patients with acute myocardial infarction (AMI) and exhibited biphasic M-shaped release kinetics with two distinct peaks. The ratio was elevated 2 hours after symptom onset, dropped to the lowest level at 10 hours, and rose to the second peak at 14 hours. A similar biphasic M-shaped curve was observed in AMI patients with blood samples taken prior to any intervention. Conclusions. The TBET/GATA3 mRNA ratio was elevated in AMI patients throughout most of the first 20 hours after symptom onset. The biphasic M-shaped release kinetics was more likely to reflect pathophysiological changes rather than treatment effects. 1. Introduction On activation, T lymphocytes differentiate into T-helper (Th) 1 and Th2 subsets, and although the control of the Th1/Th2 imbalance is not fully elucidated, there is growing evidence to suggest that two transcription factors, T-box expressed in T cells (TBET) and guanine adenine thymine adenine sequence-binding protein 3 (GATA3), play important roles in such differentiation [1–4]. Th1 is essential in the process of plaque instability and plaque rupture, which in turn are common features in the pathogenesis of acute coronary syndrome (ACS) [5–7]. Upregulation of Th1 response has been demonstrated in the circulating lymphocytes of patients with ACS [6, 8]. In contrast, Th2 has rarely been shown in atherosclerotic lesions. Recent studies suggest that loss of Th1 and Th2 balance contributes to plaque rupture and the onset of ACS [6, 9]. Th1 and Th2 imbalances in the pathogenesis of ACS have been observed coherently from transcription to protein levels in both animal models and human subjects [8–13]. The relative expression of TBET and GATA3, resulting in a swing in the Th1/Th2 pendulum, has been implicated in several immunological diseases and may provide better prognostic and diagnostic information than downstream cytokines. In our recent
References
[1]
W.-P. Zheng and R. A. Flavell, “The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells,” Cell, vol. 89, no. 4, pp. 587–596, 1997.
[2]
S. J. Szabo, S. T. Kim, G. L. Costa, X. Zhang, C. G. Fathman, and L. H. Glimcher, “A novel transcription factor, T-bet, directs Th1 lineage commitment,” Cell, vol. 100, no. 6, pp. 655–669, 2000.
[3]
K. M. Murphy and S. L. Reiner, “The lineage decisions of helper T cells,” Nature Reviews Immunology, vol. 2, no. 12, pp. 933–944, 2002.
[4]
S. J. Szabo, B. M. Sullivan, S. L. Peng, and L. H. Glimcher, “Molecular mechanisms regulating Th1 immune responses,” Annual Review of Immunology, vol. 21, pp. 713–758, 2003.
[5]
E. Laurat, B. Poirier, E. Tupin et al., “In vivo downregulation of T helper cell 1 immune responses reduces atherogenesis in apolipoprotein E-knockout mice,” Circulation, vol. 104, no. 2, pp. 197–202, 2001.
[6]
H. Methe, S. Brunner, D. Wiegand, M. Nabauer, J. Koglin, and E. R. Edelman, “Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes,” Journal of the American College of Cardiology, vol. 45, no. 12, pp. 1939–1945, 2005.
[7]
M. Fisher and E. Folland, “Acute ischemic coronary artery disease and ischemic stroke: similarities and differences,” American Journal of Therapeutics, vol. 15, no. 2, pp. 137–149, 2008.
[8]
Q.-W. Ji, M. Guo, J.-S. Zheng et al., “Downregulation of T helper cell type 3 in patients with acute coronary syndrome,” Archives of Medical Research, vol. 40, no. 4, pp. 285–293, 2009.
[9]
H. Soejima, A. Irie, S. Miyamoto et al., “Preference toward a T-helper type 1 response in patients with coronary spastic angina,” Circulation, vol. 107, no. 17, pp. 2196–2200, 2003.
[10]
G. B. Sajan, T. Z. Qiu, X. Wang, and H.-P. Guo, “T helper cell related interleukins and the angiographic morphology in unstable angina,” Cytokine, vol. 30, no. 5, pp. 303–310, 2005.
[11]
H. Yamashita, K. Shimada, E. Seki, H. Mokuno, and H. Daida, “Concentrations of interleukins, interferon, and C-reactive protein in stable and unstable angina pectoris,” American Journal of Cardiology, vol. 91, no. 2, pp. 133–136, 2003.
[12]
X. Cheng, Y.-H. Liao, H. Ge et al., “Th1/Th2 functional imbalance after acute myocardial infarction: coronary arterial inflammation or myocardial inflammation,” Journal of Clinical Immunology, vol. 25, no. 3, pp. 246–253, 2005.
[13]
A. Adler, Y. Levy, A. Roth, D. Wexler, G. Keren, and J. George, “Functional T-lymphocyte dichotomy in the peripheral blood of patients with unstable angina,” International Journal of Cardiovascular Interventions, vol. 7, no. 3, pp. 146–151, 2005.
[14]
T. H. Rainer, R. W. Y. Chan, C. A. Graham et al., “Circulating leukocyte TBET and GATA3 mRNA in patients with acute coronary syndrome,” International Journal of Cardiology, vol. 156, no. 2, pp. 209–211, 2012.
[15]
J. L. Anderson, C. D. Adams, E. M. Antman et al., “ACC/AHA 2007 guidelines for the management of patients with unstable angina/non-ST-Elevation myocardial infarction: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Writing Committee to Revise the 2002 Guidelines for the Management of Patients With Unstable Angina/Non-ST-Elevation Myocardial Infarction) developed in collaboration with the American College of Emergency Physicians, the Society for Cardiovascular Angiography and Interventions, and the Society of Thoracic Surgeons endorsed by the American Association of Cardiovascular and Pulmonary Rehabilitation and the Society for Academic Emergency Medicine,” Journal of the American College of Cardiology, vol. 50, no. 7, pp. e1–e157, 2007.
[16]
K. Thygesen, J. S. Alpert, and H. D. White, “Joint ESC/ACCF/AHA/WHF task force for the redefinition of myocardial infarction. Universal definition of myocardial infarction2007,” Journal of the American College of Cardiology, vol. 50, pp. 2173–2195, 2007.
[17]
E. M. Antman, D. T. Anbe, P. W. Armstrong et al., “ACC/AHA guidelines for the management of patients with ST-elevation myocardial infarction: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Committee to Revise the 1999 Guidelines for the Management of Patients with Acute Myocardial Infarction),” Circulation, vol. 110, no. 9, pp. e82–e292, 2004.
[18]
G. W. Snedecor and W. G. Cochran, Statistical Methods, The Iowa State University Press, Ames, Iowa, USA, 6th edition, 1971.
[19]
G. K. Hansson, “Immune mechanisms in atherosclerosis,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 21, pp. 1876–1890, 2001.
[20]
P. Libby, “Current concepts of the pathogenesis of the acute coronary syndromes,” Circulation, vol. 104, no. 3, pp. 365–372, 2001.
[21]
G. J. Blake and P. M. Ridker, “Novel clinical markers of vascular wall inflammation,” Circulation Research, vol. 89, no. 9, pp. 763–771, 2001.
[22]
L. Lind, “Circulating markers of inflammation and atherosclerosis,” Atherosclerosis, vol. 169, no. 2, pp. 203–214, 2003.
[23]
L. M. Biasucci, “CDC/AHA Workshop on Markers of Inflammation and Cardiovascular Disease: application to Clinical and Public Health Practice: clinical use of inflammatory markers in patients with cardiovascular diseases: a background paper,” Circulation, vol. 110, no. 25, pp. e560–e567, 2004.
[24]
S. J. Szabo, B. M. Sullivan, C. Sternmann, A. R. Satoskar, B. P. Sleckman, and L. H. Glimcher, “Distinct effects of T-bet in Th1 lineage commitment and IFN-γ production in CD4 and CD8 T cells,” Science, vol. 295, no. 5553, pp. 338–342, 2002.
[25]
P. R. Moreno, E. Falk, I. F. Palacios, J. B. Newell, V. Fuster, and J. T. Fallon, “Macrophage infiltration in acute coronary syndromes: implications for plaque rupture,” Circulation, vol. 90, no. 2, pp. 775–778, 1994.
[26]
E. Lantelme, S. Mantovani, B. Palermo, R. Campanelli, F. Sallusto, and C. Giachino, “Kinetics of GATA-3 gene expression in early polarizing and committed human T cells,” Immunology, vol. 102, no. 2, pp. 123–130, 2001.
[27]
H. J. Lee, N. Takemoto, H. Kurata et al., “GATA-3 induces T helper cell type 2 (Th2) cytokine expression and chromatin remodeling in committed Th1 cells,” Journal of Experimental Medicine, vol. 192, no. 1, pp. 105–115, 2000.
[28]
J. L. Fernandes, R. L. Mamoni, J. L. Orford et al., “Increased Th1 activity in patients with coronary artery disease,” Cytokine, vol. 26, no. 3, pp. 131–137, 2004.
[29]
H. Chakir, H. Wang, D. E. Lefebvre, J. Webb, and F. W. Scott, “T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: Predominant role of GATA-3,” Journal of Immunological Methods, vol. 278, no. 1-2, pp. 157–169, 2003.