An RP-HPLC method for simultaneous determination of 9 NSAIDs (paracetamol, salicylic acid, ibuprofen, naproxen, aceclofenac, diclofenac, ketorolac, etoricoxib, and aspirin) and their commonly prescribed combination drugs (thiocolchicoside, moxifloxacin, clopidogrel, chlorpheniramine maleate, dextromethorphan, and domperidone) was established. The separation was performed on Kromasil C18 (250 × 4.6？mm, 5？μm) at 35°C using 15？mM phosphate buffer pH 3.25 and acetonitrile with gradient elution at a flow rate of 1.1？mL/min. The detection was performed by a diode array detector (DAD) at 230？nm with total run time of 30？min. Calibration curves were linear with correlation coefficients of determination . Limit of detection (LOD) and Limit of quantification (LOQ) ranged from 0.04 to 0.97？μg/mL and from 0.64 to 3.24？μg/mL, respectively. As an application tool of quality by design, full factorial experimental design was used for the testing of robustness of the method. The prediction profiler correlating various parameters and responses was established from the results of design of experiments (DOE). 1. Introduction Nonsteroidal anti-inflammatory drugs (NSAIDs) are among the most frequently prescribed drugs worldwide and are used for relief of inflammatory, chronic (e.g., rheumatoid arthritis, osteoarthritis, and gout), and acute (e.g., headache, postoperative pain, and orthopedic fractures) pain conditions [1]. NSAIDs formulations are also available as over-the counter pharmaceutical preparations. The anti-inflammatory activity of NSAIDs and most of their other pharmacological effects are related to the inhibition of the conversion of arachidonic acid to prostaglandins, which are mediators of the inflammatory process. NSAIDs are potent inhibitors of cyclooxygenase in vitro and in vivo, thereby decreasing the synthesis of prostaglandins, prostacyclin, and thromboxane products [2]. Table 1 represents the classification of NSAIDs based on their chemical structure [3]. Table 1: Chemical classification of NSAIDs. The growing demand for NSAIDs stimulates higher level of quality control of these therapeutic substances and preparations. Hence, there is need to develop new analytical methods for qualitative and quantitative analysis of NSAIDs and their combination drugs. There are a number of liquid chromatographic methods reported in the literature [4–52] for the individual assays of these drugs and also for the some of their combinations but so far no method has been reported for the simultaneous determination of cited NSAIDs in the presence of selected commonly
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