Muscle hypertrophy following resistance training (RT) involves activation of myofibrillar protein synthesis (MPS) to expand the myofibrillar protein pool. The degree of hypertrophy following RT is, however, highly variable and thus we sought to determine the relationship between the acute activation of MPS and RT-induced hypertrophy. We measured MPS and signalling protein activation after the first session of resistance exercise (RE) in untrained men (n = 23) and then examined the relation between MPS with magnetic resonance image determined hypertrophy. To measure MPS, young men (24±1 yr; body mass index = 26.4±0.9 kg？m2) underwent a primed constant infusion of L-[ring-13C6] phenylalanine to measure MPS at rest, and acutely following their first bout of RE prior to 16 wk of RT. Rates of MPS were increased 235±38% (P<0.001) above rest 60–180 min post-exercise and 184±28% (P = 0.037) 180–360 min post exercise. Quadriceps volume increased 7.9±1.6% (？1.9–24.7%) (P<0.001) after training. There was no correlation between changes in quadriceps muscle volume and acute rates of MPS measured over 1–3 h (r = 0.02), 3–6 h (r = 0.16) or the aggregate 1–6 h post-exercise period (r = 0.10). Hypertrophy after chronic RT was correlated (r = 0.42, P = 0.05) with phosphorylation of 4E-BP1Thr37/46 at 1 hour post RE. We conclude that acute measures of MPS following an initial exposure to RE in novices are not correlated with muscle hypertrophy following chronic RT.
References
[1]
Terzis G, Georgiadis G, Stratakos G, Vogiatzis I, Kavouras S, et al. (2008) Resistance exercise-induced increase in muscle mass correlates with p70S6 kinase phosphorylation in human subjects. Eur J Appl Physiol 102: 145–152. doi: 10.1007/s00421-007-0564-y
[2]
Mitchell CJ, Churchward-Venne TA, West DW, Burd NA, Breen L, et al. (2012) Resistance exercise load does not determine training-mediated hypertrophic gains in young men. J Appl Physiol 113: 71–77. doi: 10.1152/japplphysiol.00307.2012
[3]
Phillips BE, Williams JP, Gustafsson T, Bouchard C, Rankinen T, et al. (2013) Molecular Networks of Human Muscle Adaptation to Exercise and Age. PLoS Genet 9: e1003389. doi: 10.1371/journal.pgen.1003389
[4]
Wilkinson SB, Tarnopolsky MA, Macdonald MJ, Macdonald JR, Armstrong D, et al. (2007) Consumption of fluid skim milk promotes greater muscle protein accretion after resistance exercise than does consumption of an isonitrogenous and isoenergetic soy-protein beverage. Am J Clin Nutr 85: 1031–1040. doi: 10.1016/s0162-0908(08)79214-6
[5]
Tang JE, Moore DR, Kujbida GW, Tarnopolsky MA, Phillips SM (2009) Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol 107: 987–992. doi: 10.1152/japplphysiol.00076.2009
[6]
Hartman JW, Tang JE, Wilkinson SB, Tarnopolsky MA, Lawrence RL, et al. (2007) Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters. Am J Clin Nutr 86: 373–381. doi: 10.1016/s0162-0908(08)79232-8
[7]
Burd NA, West DW, Staples AW, Atherton PJ, Baker JM, et al. (2010) Low-load high volume resistance exercise stimulates muscle protein synthesis more than high-load low volume resistance exercise in young men. PLoS One 5: e12033. doi: 10.1371/journal.pone.0012033
[8]
Burd NA, Holwerda AM, Selby KC, West DW, Staples AW, et al. (2010) Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. J Physiol 588: 3119–3130. doi: 10.1113/jphysiol.2010.192856
[9]
Petrella JK, Kim JS, Mayhew DL, Cross JM, Bamman MM (2008) Potent myofiber hypertrophy during resistance training in humans is associated with satellite cell-mediated myonuclear addition: a cluster analysis. J Appl Physiol 104: 1736–1742. doi: 10.1152/japplphysiol.01215.2007
[10]
Davidsen PK, Gallagher IJ, Hartman JW, Tarnopolsky MA, Dela F, et al. (2011) High responders to resistance exercise training demonstrate differential regulation of skeletal muscle microRNA expression. J Appl Physiol 110: 309–317. doi: 10.1152/japplphysiol.00901.2010
[11]
West DW, Phillips SM (2012) Associations of exercise-induced hormone profiles and gains in strength and hypertrophy in a large cohort after weight training. Eur J Appl Physiol 112: 2693–2702. doi: 10.1007/s00421-011-2246-z
[12]
Mitchell CJ, Churchward-Venne TA, Bellamy L, Parise G, Baker SK, et al. (2013) Muscular and systemic correlates of resistance training-induced muscle hypertrophy. PLoS One 8: e78636. doi: 10.1371/journal.pone.0078636
[13]
Mayhew DL, Kim JS, Cross JM, Ferrando AA, Bamman MM (2009) Translational signaling responses preceding resistance training-mediated myofiber hypertrophy in young and old humans. J Appl Physiol 107: 1655–1662. doi: 10.1152/japplphysiol.91234.2008
[14]
Burd NA, Yang Y, Moore DR, Tang JE, Tarnopolsky MA, et al. (2012) Greater stimulation of myofibrillar protein synthesis with ingestion of whey protein isolate v. micellar casein at rest and after resistance exercise in elderly men. Br J Nutr 108: 958–962. doi: 10.1017/s0007114511006271
[15]
Churchward-Venne TA, Burd NA, Mitchell CJ, West DW, Philp A, et al. (2012) Supplementation of a suboptimal protein dose with leucine or essential amino acids: effects on myofibrillar protein synthesis at rest and following resistance exercise in men. J Physiol 590: 2751–2765. doi: 10.1113/jphysiol.2012.228833
[16]
West DW, Kujbida GW, Moore DR, Atherton P, Burd NA, et al. (2009) Resistance exercise-induced increases in putative anabolic hormones do not enhance muscle protein synthesis or intracellular signalling in young men. J Physiol 587: 5239–5247. doi: 10.1113/jphysiol.2009.177220
[17]
Burd NA, Groen BB, Beelen M, Senden JM, Gijsen AP, et al. (2012) The reliability of using the single-biopsy approach to assess basal muscle protein synthesis rates in vivo in humans. Metabolism 61: 931–936. doi: 10.1016/j.metabol.2011.11.004
[18]
Burd NA, Pennings B, Groen BB, Gijsen AP, Senden JM, et al. (2012) The single biopsy approach is reliable for the measurement of muscle protein synthesis rates in vivo in older men. J Appl Physiol 113: 896–902. doi: 10.1152/japplphysiol.00513.2012
[19]
Cermak NM, Res PT, de Groot LC, Saris WH, van Loon LJ (2012) Protein supplementation augments the adaptive response of skeletal muscle to resistance-type exercise training: a meta-analysis. Am J Clin Nutr 96: 1454–1464. doi: 10.3945/ajcn.112.037556
[20]
Thalacker-Mercer AE, Petrella JK, Bamman MM (2009) Does habitual dietary intake influence myofiber hypertrophy in response to resistance training? A cluster analysis. Appl Physiol Nutr Metab 34: 632–639. doi: 10.1139/h09-038
[21]
Phillips SM, Tipton KD, Ferrando AA, Wolfe RR (1999) Resistance training reduces the acute exercise-induced increase in muscle protein turnover. Am J Physiol 276: E118–124.
[22]
Tang JE, Perco JG, Moore DR, Wilkinson SB, Phillips SM (2008) Resistance training alters the response of fed state mixed muscle protein synthesis in young men. Am J Physiol Regul Integr Comp Physiol 294: R172–178. doi: 10.1152/ajpregu.00636.2007
[23]
Phillips SM, Parise G, Roy BD, Tipton KD, Wolfe RR, et al. (2002) Resistance-training-induced adaptations in skeletal muscle protein turnover in the fed state. Can J Physiol Pharmacol 80: 1045–1053. doi: 10.1139/y02-134
[24]
Kim PL, Staron RS, Phillips SM (2005) Fasted-state skeletal muscle protein synthesis after resistance exercise is altered with training. J Physiol 568: 283–290. doi: 10.1113/jphysiol.2005.093708
[25]
Wilkinson SB, Phillips SM, Atherton PJ, Patel R, Yarasheski KE, et al. (2008) Differential effects of resistance and endurance exercise in the fed state on signalling molecule phosphorylation and protein synthesis in human muscle. J Physiol 586: 3701–3717. doi: 10.1113/jphysiol.2008.153916
[26]
Glynn EL, Fry CS, Drummond MJ, Dreyer HC, Dhanani S, et al. (2010) Muscle protein breakdown has a minor role in the protein anabolic response to essential amino acid and carbohydrate intake following resistance exercise. Am J Physiol Regul Integr Comp Physiol 299: R533–540. doi: 10.1152/ajpregu.00077.2010
[27]
Phillips SM, Tipton KD, Aarsland A, Wolf SE, Wolfe RR (1997) Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Physiol 273: E99–107.
[28]
Bodine SC, Stitt TN, Gonzalez M, Kline WO, Stover GL, et al. (2001) Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nat Cell Biol 3: 1014–1019.
[29]
Mayhew DL, Hornberger TA, Lincoln HC, Bamman MM (2011) Eukaryotic initiation factor 2B epsilon induces cap-dependent translation and skeletal muscle hypertrophy. J Physiol 589: 3023–3037. doi: 10.1113/jphysiol.2010.202432
